A5012431332- Platelet Disorders and Treatments
- Systemic Lupus Erythematosus Research
- Blood Coagulation and Thrombosis Mechanisms
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood groups and transfusion
- Venous Thromboembolism Diagnosis and Management
- Hemophilia Treatment and Research
- Antiplatelet Therapy and Cardiovascular Diseases
- Heparin-Induced Thrombocytopenia and Thrombosis
- Blood properties and coagulation
- Complement system in diseases
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Blood disorders and treatments
- Parasite Biology and Host Interactions
- Diabetes and associated disorders
- Parasites and Host Interactions
- Atrial Fibrillation Management and Outcomes
- Hemoglobinopathies and Related Disorders
- Streptococcal Infections and Treatments
- Coronary Interventions and Diagnostics
- Blood transfusion and management
- Immune Response and Inflammation
- Immune Cell Function and Interaction
Utrecht University
2015-2025
University Medical Center Utrecht
2016-2025
University of North Carolina at Chapel Hill
2023
Karolinska Institutet
2023
Centro Hospitalar Lisboa Norte
2023
University of Groningen
2022
Erasmus MC
2019-2022
Erasmus MC - Sophia Children’s Hospital
2022
De Forensische Zorgspecialisten
2020
University Medical Center
2019
Blood-based liquid biopsies, including tumor-educated blood platelets (TEPs), have emerged as promising biomarker sources for non-invasive detection of cancer. Here we demonstrate that particle-swarm optimization (PSO)-enhanced algorithms enable efficient selection RNA panels from platelet RNA-sequencing libraries (n = 779). This resulted in accurate TEP-based early- and late-stage non-small-cell lung cancer 518 validation cohort, accuracy, 88%; AUC, 0.94; 95% CI, 0.92–0.96; p < 0.001; n 106...
In antiphospholipid syndrome (APS), antibodies (aPL) binding to β2 glycoprotein I (β2GPI) induce endothelial cell-leukocyte adhesion and thrombus formation via unknown mechanisms. Here we show that in mice both of these processes are caused by the inhibition eNOS. studies cultured human, bovine, mouse cells, promotion monocyte aPL entailed decreased bioavailable NO, fully antagonized eNOS activation diverse agonists. Similarly, NO-dependent, acetylcholine-induced increases carotid vascular...
Binding of activated protein C (APC) to cells triggers multiple beneficial cytoprotective activities that suppress apoptosis, inflammation, and endothelial barrier breakdown. One paradigm for APC's signaling emphasizes its binding cell receptor (EPCR) subsequent protease (PAR)-1 activation. Here we used human monocytic-like U937 evaluate apolipoprotein E 2 (ApoER2)-dependent by APC found initiated rapid phosphorylation Tyr-220 in the adaptor disabled-1 (Dab1) Ser-473 Akt. also induced Ser-9...
The presence of autoantibodies against a cryptic epitope in domain I β(2)-glycoprotein (β(2)GPI) is strongly associated with thrombotic events patients the antiphospholipid syndrome. We hypothesized that conformational change could be trigger for formation antibodies β(2)GPI. Therefore, we investigated whether immune responses β(2)GPI are related to its conformation.Conformational changes were studied using various techniques, either upon binding cardiolipin or after disruption internal...
ABSTRACT The collagenous C-type lectin, SP-D, is a multitrimeric glycoprotein present at mucosal surfaces and involved in host defense against infections mammals. SP-D has immunomodulatory properties, but the underlying mechanisms are incompletely understood. contains collagen domains. LAIR-1 an inhibitory immune receptor cell surface of various immune-competent cells that binds collagen. We hypothesized functions can be mediated via interactions between its domain LAIR-1. Binding assays...
Objective Pregnancies in women with the antiphospholipid syndrome (APS) are frequently complicated by fetal loss and intrauterine growth restriction (IUGR). How circulating antibodies (aPL) cause pregnancy complications APS is poorly understood. We sought to determine whether low‐density lipoprotein receptor family member apolipoprotein E 2 (ApoER2) mediates trophoblast dysfunction induced aPL. Methods Placental ApoER2 expression was evaluated immunohistochemistry immunoblotting. Normal...
Inherited bleeding disorders such as Glanzmann thrombasthenia (GT) lack prophylactic treatment options. As a result, serious episodes are treated acutely with blood product transfusions or frequent, repeated intravenous administration of recombinant activated coagulation factor VII (rFVIIa). Here we describe HMB-001, bispecific antibody designed to bind and accumulate endogenous FVIIa deliver it sites vascular injury by targeting the TREM (triggering receptor expressed on myeloid cells)-like...
Blood coagulation functions as part of the innate immune system by preventing bacterial invasion, and it is critical to stopping blood loss (hemostasis). Coagulation involves external membrane surface activated platelets leukocytes. Using lipidomic, genetic, biochemical, mathematical modeling approaches, we found that enzymatically oxidized phospholipids (eoxPLs) generated activity leukocyte or platelet lipoxygenases (LOXs) were required for normal hemostasis promoted factor activities in a...
Essentials•The diagnosis of mild platelet function disorders (PFDs) is challenging.•Validation flow cytometric testing in patients with suspected PFDs required.•Flow cytometry has added value to light transmission aggregometry (LTA) PFDs.•There fair agreement diagnosing between LTA and cytometry.Summary: BackgroundLight the most commonly used test for (PFDs), but moderate sensitivity PFDs. Flow been recommended additional diagnostics not yet standardized as a diagnostic test. We developed...
Thrombocytopenia and platelet dysfunction are commonly observed in patients with dengue virus (DENV) infection may contribute to complications such as bleeding plasma leakage. The etiology of dengue-associated thrombocytopenia is multifactorial includes increased clearance. binding the coagulation protein von Willebrand factor (VWF) membrane removal sialic acid (desialylation) two well-known mechanisms clearance, but whether these conditions also unknown. In observational cohort studies...
Background The presence of lupus anticoagulant (LA) is an independent risk factor for thrombosis. This laboratory phenomenon detected as a phospholipid-dependent prolongation the clotting time and caused by autoantibodies against beta2-glycoprotein I (β2GPI) or prothrombin. How these cause LA unclear. Objective To elucidate how anti-β2GPI anti-prothrombin antibodies phenomenon. Methods effects monoclonal on coagulation were analyzed in plasma with purified factors. Results Detection required...
Delta storage pool disease (δ-SPD) is a rare platelet function disorder (PFD) characterized by deficiency of dense granules or defective granule secretion, leading to bleeding diathesis. Diagnostics δ-SPD are difficult and lack standardization, underestimation its prevalence. Current diagnostic methods based on content assays lumi-aggregometry, which have limited availability. Therefore, there an unmet need for rapid, accessible test δ-SPD. To evaluate the value rapid whole blood ATP...