A5035618422- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Escherichia coli research studies
- CAR-T cell therapy research
- Bacteriophages and microbial interactions
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Animal testing and alternatives
- Asthma and respiratory diseases
- Platelet Disorders and Treatments
- Immunotherapy and Immune Responses
- Fibroblast Growth Factor Research
- Tuberculosis Research and Epidemiology
- Cancer Research and Treatments
- Mycobacterium research and diagnosis
- Connective tissue disorders research
- Adipose Tissue and Metabolism
- Neurotransmitter Receptor Influence on Behavior
- Psoriasis: Treatment and Pathogenesis
- Wnt/β-catenin signaling in development and cancer
- Herpesvirus Infections and Treatments
- Bat Biology and Ecology Studies
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Dermatological diseases and infestations
- Parkinson's Disease Mechanisms and Treatments
University of Washington
2022
Bristol-Myers Squibb (United States)
2012-2017
HistoGenetics (United States)
2002-2008
Stanford University
1997-1999
Johns Hopkins University
1993-1995
Johns Hopkins Bayview Medical Center
1994-1995
Johns Hopkins Medicine
1993
National Institutes of Health
1990-1992
Office of Research Services
1990
University of Missouri
1987-1990
CD40-CD40L interactions play a critical role in regulating immune responses. Blockade of CD40L by Abs, such as the anti-CD40L Ab 5c8, demonstrated positive clinical effects patients with autoimmune diseases; however, incidents thromboembolism (TE) precluded further development these molecules. In this study, we examined Fc domain interaction FcγRs modulating platelet activation and potential for TE. Our results show that 5c8 wild-type IgG1 is responsible activation, measured induction PAC-1...
Recent advances in cancer treatment with checkpoint blockade of receptors such as CTLA-4 and PD-1 have demonstrated that combinations agents complementary immunomodulatory effects the potential to enhance antitumor activity compared single agents. We investigated efficacy immune-modulatory interleukin-21 (IL-21) combined several syngeneic mouse tumor models. After establishment, mice were administered recombinant IL-21 (mIL-21) alone or combination blocking monoclonal antibodies against...
Corticotroph-derived glycoprotein hormone (CGH), also referred to as thyrostimulin, is a noncovalent heterodimer of alpha 2 (GPHA2) and beta 5 (GPHB5). Here, we demonstrate that both subunits CGH are expressed in the corticotroph cells human anterior pituitary, well skin, retina, testis. activates TSH receptor (TSHR); (125)I-CGH binding expressing TSHR saturable, specific, high affinity. In competition studies, unlabeled potent competitor for (125)I-TSH binding, whereas does not compete...
Abstract Binding of Ag-Ab immune complexes to cellular FcγR promotes cell activation, release inflammatory mediators, and tissue destruction characteristic autoimmune disease. To evaluate whether a soluble could block the proinflammatory effects complexes, recombinant human (rh) versions FcγRIA, FcγRIIA, FcγRIIIA were prepared. rh-FcγRIA IgG was high affinity (KD = 1.7 × 10−10 M), whereas rh-FcγRIIA rh-FcγRIIIA bound with low 0.6–1.9 10−6 M). All rh-FcγR reduced complex precipitation,...
Binding of immune complexes to cellular FcgammaRs can promote cell activation and inflammation. In previous studies, a recombinant human (rh) soluble FcgammaR, rh-FcgammaRIA (CD64A), was shown block inflammation in passive transfer models complex-mediated disease. To assess whether could T cell- B cell-dependent model disease, the efficacy collagen-induced arthritis evaluated. Mice with established were treated single s.c. injection (0.2-2.0 mg/dose) given every other day for 11 days....
Protein and antigenic heterogeneity among isolates of Bacillus piliformis, the etiologic agent Tyzzer's disease, were investigated. The seven utilized in this study originally isolated from naturally infected animals different animal species diverse geographical locations. Isolates propagated mammalian cell lines, bacterial extracts prepared. profiles compared isolates, using Coomassie blue-stained polyacrylamide gels Western blot (immunoblot) analyses, respectively. Results showed...
Increased levels of serum IgE are associated with greater asthma prevalence and disease severity. depletion using an anti-IgE monoclonal antibody has met success in the treatment moderate-to-severe severe persistent allergic asthma.To test whether B cell-targeted therapy is a more effective for airway hyperresponsiveness (AHR) murine model compared IgE-depletion.We delivered soluble mTACI-Ig, receptor cell survival factors BLyS (B Lymphocyte Stimulator) APRIL (A PRoliferation-Inducing...
Interleukin-21 (IL-21), a pleiotropic immunostimulatory type I cytokine, has anticancer effects in animal models. Preclinical studies designed to assess the safety of recombinant human IL-21 (rIL-21) for use phase oncology are described. The rIL-21 (≤3.0 mg/kg per dose) was given intravenously cynomolgus monkeys ( Macaca fascicularis) once daily 5 days, followed by 9 nondosing days (1 cycle) ≤4 cycles. pharmacokinetics dose-dependent. Accumulation not observed after repeated dosing,...
The relative susceptibilities of C57BL/6NCR and BALB/cANNCR mice, F344/NCR rats, 2/NCR guineapigs CR:RGH Syrian hamsters to Bacillus piliformis infection were determined by orally inoculating 20 weanling females from each species with suspensions B. spores. Animals group sequentially necropsied over 2 week periods document the lesions produced. No significant gross or microscopic observed in BALB mice Fischer rats. Gross livers intestines many killed 3–14 days after inoculation. A large...
Factor XIII (FXIII) is a thrombin-activated plasma coagulation factor critical for blood clot stabilization and longevity. Administration of exogenous FXIII to replenish depleted stores after major surgery, including cardiopulmonary bypass, may reduce bleeding complications transfusion requirements. Thus, model extracorporeal circulation (ECC) was developed in adult male cynomolgus monkeys ( Macaca fascicularis) evaluate the nonclinical safety recombinant human (rFXIII). The hematological...
Male Wistar rats aged 6-26 months were obtained from the colony of The Gerontology Research Center National Institute on Aging, and pathological profiles assessed. One hundred animals sacrificed at 6, 12, 18, 21, 24, 26 used for cross-sectional determinations; also, 150 followed longitudinally when clinical signs moribundity appeared. Renal disease contributed most common pathology observed in both studies (found over 70% examined), with neoplasms a secondary problem (pituitary tumors by far...
An undescribed species of Demodex (Acari: Demodecidae) was observed in hair follicles and ducts sebaceous glands the ear canals seven California ground squirrels (Spermophilus beecheyi) from Santa Clara Count); (USA). The animals had died unrelated causes were submitted for necropsy between September 1994 February 1996. Similar mites lumens Meibomian eyelids two these squirrels. Microscopic changes epithelium surrounding dermis, when present, minimal. No associated clinical signs disease or...
The relationship between hematological variables and the ability to perform behaviorally in two learning tests was evaluated male F344 rats aged 22-24 months. Rats were screened for meet criterion one-way active avoidance a straight runway task. failing given no further testing assigned Group 1 (G1). meeting tested 14-unit T-maze (2 days, 10 trials/day). Failure negotiate within 600 s on any three trials resulted assignment 2 (G2) with testing. successfully completing both tasks constituted...
2598 Background: Interleukin 21 (IL-21), a member of the IL-2 cytokine family, is produced by activated CD4+ T cells. We have previously shown recombinant murine IL-21 (mIL-21) to be both efficacious and well tolerated in mouse B16F10 melanoma model. Efficacy this model likely mediated through enhancement NK cell cytotoxicity, possibly increased survival tumor-specific CTLs. The current study examined efficacy mIL-21 mice bearing renal carcinoma tumors (Renca). Methods: 80 Balb/c were...