A5023936366- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA Research and Splicing
- MicroRNA in disease regulation
- RNA modifications and cancer
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Immune cells in cancer
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Telomeres, Telomerase, and Senescence
- Cancer Cells and Metastasis
- EEG and Brain-Computer Interfaces
- Artificial Intelligence in Healthcare
- Regulation of Appetite and Obesity
- Inflammasome and immune disorders
- Berberine and alkaloids research
- Muscle Physiology and Disorders
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Genetic Neurodegenerative Diseases
- Metabolism, Diabetes, and Cancer
- Plant and Fungal Interactions Research
- Liver Disease Diagnosis and Treatment
- Immune Cell Function and Interaction
National Institutes of Health
2018-2024
National Institute on Aging
2018-2024
Institute on Aging
2018-2024
Centre For Human Genetics
2024
Krea University
2024
Columbia University
2024
The University of Texas MD Anderson Cancer Center
2022
National Centre for Cell Science
2014-2020
Savitribai Phule Pune University
2016-2020
Abstract By interacting with proteins and nucleic acids, the vast family of mammalian circRNAs is proposed to influence many biological processes. Here, RNA sequencing analysis differentially expressed during myogenesis revealed that circSamd4 expression increased robustly in mouse C2C12 myoblasts differentiating into myotubes. Moreover, silencing circSamd4, which conserved between human mouse, delayed lowered myogenic markers cultured from both species. Affinity pulldown followed by mass...
The senescence-associated secretory phenotype (SASP) is a major trait of senescent cells, but the molecular regulators SASP factor secretion are poorly understood. Mass spectrometry analysis revealed that carrier membrane protein 4 (SCAMP4) levels were strikingly elevated on surface cells compared with proliferating cells. Interestingly, silencing SCAMP4 in fibroblasts reduced factors, including interleukin 6 (IL6), IL8, growth differentiation 15 (GDF-15), C-X-C motif chemokine ligand 1...
Abstract Long noncoding (lnc)RNAs potently regulate gene expression programs in physiology and disease. Here, we describe a key function for lncRNA OIP5-AS1 myogenesis, the process whereby myoblasts differentiate into myotubes during muscle development regeneration after injury. In human myoblasts, levels increased robustly early its loss attenuated myogenic differentiation reduced of transcription factor MEF2C. This effect relied upon partial complementarity with MEF2C mRNA presence HuR, an...
Abstract Malignant characteristics of cancers, represented by rapid cell proliferation and high metastatic potential, are a major cause cancer-related mortality. As multifunctional RNA-binding protein, heterogeneous nuclear ribonucleoprotein K (hnRNPK) is closely associated with cancer progression in various types cancers. In this study, we sought to identify hnRNPK-regulated long intergenic non-coding RNAs (lincRNAs) that play critical role the regulation malignancy. We found hnRNPK...
Aging | doi:10.18632/aging.203170. Rachel Munk, Carlos Anerillas, Martina Rossi, Dimitrios Tsitsipatis, Jennifer L. Martindale, Allison B. Herman, Jen-Hao Yang, Jackson A. Roberts, Vijay R. Varma, Poonam Pandey, Madhav Thambisetty, Myriam Gorospe, Kotb Abdelmohsen
Abstract Long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) modulate gene expression programs in physiology disease. Here, we report a RNA regulatory network that modulates myoblast fusion into multinucleated myotubes, process occurs during muscle development regeneration after injury. In early stages of human myogenesis, the levels lncRNA OIP5-AS1 increased, while miR-7 decreased. Moreover, bound induced decay via target RNA-directed miRNA decay; accordingly, loss attenuated, antagonizing...
The RNA-binding protein GRSF1 (G-rich RNA sequence-binding factor 1) critically maintains mitochondrial homeostasis. Accordingly, loss of impaired respiration and increased the levels reactive oxygen species (ROS), triggering DNA damage, growth suppression, a senescent phenotype characterized by elevated production secretion interleukin (IL)6. Here, we characterize pathways that govern IL6 in response to dysfunction GRSF1-depleted cells. We report broadly altered expression programs,...
The mammalian transcriptome comprises a vast family of long noncoding (lnc)RNAs implicated in physiologic processes such as myogenesis, through which muscle forms during embryonic development and regenerates the adult. However, specific molecular mechanisms by lncRNAs regulate human myogenesis are poorly understood. Here, we identified novel muscle-specific lncRNA, lncFAM71E1-2:2 (lncFAM), increased robustly early myogenesis. Overexpression lncFAM promoted differentiation myoblasts into...
Abstract There is a growing imperative to understand the neurophysiological impact of our rapidly changing and diverse technological, social, chemical, physical environments. To untangle multidimensional interacting effects requires data at scale across populations, taking measurement out controlled lab environment into field. Electroencephalography (EEG), which has correlates with various environmental factors as well cognitive mental health outcomes, advantage both portability...
Insulin maintains glucose homeostasis by stimulating uptake from extracellular environment to adipose and muscle tissue through transporter (GLUT4). resistance plays a significant role in pathologies associated with type2 diabetes. It has been previously shown that hyperinsulinemia can lead insulin resistance. In these studies very high levels of was used achieve We hypothesized one the causes type 2 diabetes could be synthesis absence stimulation. CHO cell line, stably expressing...
Abstract Growing evidence indicates that long intergenic noncoding RNAs play an important role in cancer progression by affecting gene regulation at the transcriptional and posttranscriptional levels. Recent studies have shown RNA functions as a competitive endogenous RNA, which can interact with mitigate function of microRNA. In this study, we investigated molecular mechanism LINC00162 regulates cell proliferation apoptotic death. By analyzing sequencing data, was identified to be target...
Understanding the regulation of insulin biosynthesis is important as it plays a central role in glucose metabolism. The mouse gene2 (Ins2) has two splice variants; long (Ins2L) and short (Ins2S), that differ only their 5'UTR sequence Ins2S major transcript which translate more efficiently compared to Ins2L. Here, we show cellular factors bind preferentially Ins2L 5'UTR, PABP HuD can Ins2 variants regulate its translation. In vitro binding assay with different isoforms indicate 'N' terminal...
Sangiao-Alvarellos et al., 2014, have also shown that miR-200a regulate the IRS2 expression [3].Taken together these results suggest may play an important role in energy metabolism by regulating leptin and insulin signalling.The levels of ObRb are reduced Ob/Ob mice blocking intracerebro-ventricular (ICV) infusion anti restores both ObRb.Blocking mice, increased receptor mRNA coding for POMC Neuropeptide Y.The mir-200a causes reduction body weight gain however was not accompanied food intake...
Myogenesis is a highly orchestrated process whereby muscle precursor cells, myoblasts, develop into fibers to form skeletal during embryogenesis and regenerate adult muscle. Here, we studied the RNA-binding protein FUS (fused in sarcoma), which has been implicated muscular neuromuscular pathologies but poorly characterized myogenesis. Given that levels declined human mouse models of myogenesis, silencing enhanced hypothesized might be repressor myogenic differentiation. Interestingly,...
Human malignant melanoma is highly aggressive and metastatic in nature exhibits phenotypic plasticity. Melanoma has multiple phenotypically distinct subpopulations which involved tumor progression markedly resistant to conventional therapy. Some of the subpopulation stem cells feature defined as cancer (CSCs) or initiating (TICs). Identification characterization CSCs may have therapeutic implication for combating progression. In recent study, we identified that CD133+ potentially initiation,...
Aim: To determine the prevalence of diabetes and to explore various modifiable nonmodifiable factors associated with in Ahmednagar city. Method: The data was then collected using convenient sampling method. subjects were selected from VPMH hospital, camps door survey. assessed based on assessment form including Demographic data, Socio-economic Family history, Medical Surgical Personal B.M.I., Waist Hip ratio, Diabetes Drug history human activity profile questionnaire. Result: 38.6% adult...