A5087668142- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Circular RNAs in diseases
- RNA modifications and cancer
- Plant and Fungal Interactions Research
- Skin Protection and Aging
- MicroRNA in disease regulation
- RNA regulation and disease
- Liver Disease Diagnosis and Treatment
National Institute on Aging
2020-2023
National Institutes of Health
2020-2023
Institute on Aging
2020-2023
University of Maryland, Baltimore
2022
Abstract By interacting with proteins and nucleic acids, the vast family of mammalian circRNAs is proposed to influence many biological processes. Here, RNA sequencing analysis differentially expressed during myogenesis revealed that circSamd4 expression increased robustly in mouse C2C12 myoblasts differentiating into myotubes. Moreover, silencing circSamd4, which conserved between human mouse, delayed lowered myogenic markers cultured from both species. Affinity pulldown followed by mass...
Mammalian circRNAs can influence different cellular processes by interacting with proteins and other nucleic acids. Here, we used ribonucleoprotein immunoprecipitation (RIP) analysis to identify systematically the associated cancer-related protein AUF1. Among AUF1 in HeLa (human cervical carcinoma) cells, focused on hsa_circ_0032434 (circPCNX), an abundant target of Overexpression circPCNX specifically interfered binding p21 (CDKN1A) mRNA, thereby promoting mRNA stability elevating...
Abstract Lipid transport and ATP synthesis are critical for the progression of non-alcoholic fatty liver disease (NAFLD), but underlying mechanisms largely unknown. Here, we report that RNA-binding protein HuR (ELAVL1) forms complexes with NAFLD-relevant transcripts. It associates intron 24 Apob pre-mRNA, 3′UTR Uqcrb , 5′UTR Ndufb6 mRNA, thereby regulating splicing mRNA translation UQCRB NDUFB6. Hepatocyte-specific knockout reduces expression APOB, UQCRB, NDUFB6 in mice, reducing lipid...
Abstract Long noncoding (lnc)RNAs potently regulate gene expression programs in physiology and disease. Here, we describe a key function for lncRNA OIP5-AS1 myogenesis, the process whereby myoblasts differentiate into myotubes during muscle development regeneration after injury. In human myoblasts, levels increased robustly early its loss attenuated myogenic differentiation reduced of transcription factor MEF2C. This effect relied upon partial complementarity with MEF2C mRNA presence HuR, an...
Abstract Changes in the transcriptomes of human tissues with advancing age are poorly cataloged. Here, we sought to identify coding and long noncoding RNAs present cultured primary skin fibroblasts collected from 82 healthy individuals across a wide spectrum (22–89 years old) who participated GESTALT (Genetic Epigenetic Signatures Translational Aging Laboratory Testing) study National Institute on Aging, NIH. Using high‐throughput RNA sequencing linear regression model, identified 1437...
Abstract Long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) modulate gene expression programs in physiology disease. Here, we report a RNA regulatory network that modulates myoblast fusion into multinucleated myotubes, process occurs during muscle development regeneration after injury. In early stages of human myogenesis, the levels lncRNA OIP5-AS1 increased, while miR-7 decreased. Moreover, bound induced decay via target RNA-directed miRNA decay; accordingly, loss attenuated, antagonizing...
The mammalian transcriptome comprises a vast family of long noncoding (lnc)RNAs implicated in physiologic processes such as myogenesis, through which muscle forms during embryonic development and regenerates the adult. However, specific molecular mechanisms by lncRNAs regulate human myogenesis are poorly understood. Here, we identified novel muscle-specific lncRNA, lncFAM71E1-2:2 (lncFAM), increased robustly early myogenesis. Overexpression lncFAM promoted differentiation myoblasts into...