A5014170975- Trace Elements in Health
- Muscle Physiology and Disorders
- Heavy Metal Exposure and Toxicity
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Orthopaedic implants and arthroplasty
- DNA and Nucleic Acid Chemistry
- Bacillus and Francisella bacterial research
- Advanced biosensing and bioanalysis techniques
- Acute Myeloid Leukemia Research
- Heavy metals in environment
- Pesticide Exposure and Toxicity
- Protein Degradation and Inhibitors
- Carcinogens and Genotoxicity Assessment
Northeastern University
2019-2024
The University of Melbourne
2024
Peter MacCallum Cancer Centre
2024
University of Massachusetts Chan Medical School
2018-2019
Metal‐regulatory transcription factor 1 (MTF1) is a conserved metal‐binding in eukaryotes that binds to DNA sequence motifs, termed metal response elements. MTF1 responds both excess and deprivation, protects cells from oxidative hypoxic stresses, required for embryonic development vertebrates. To examine the role cell differentiation, we use multiple experimental strategies [including gene knockdown (KD) mediated by small hairpin RNA clustered regularly interspaced short palindromic...
Abstract Trace elements such as copper (Cu), zinc (Zn), iron (Fe), and manganese (Mn) function enzyme cofactors second messengers in cell signaling. are emerging key regulators of differentiation development mammalian tissues including blood, brain, skeletal muscle. We previously reported an influx Cu dynamic expression metal transporters during muscle cells. Here, we demonstrate that myoblasts increase Mn, Fe Zn also occurs. Interestingly the Mn is concomitant with increased Mn-dependent...
Sulfur mustard (SM) is a highly reactive organic chemical has been used as warfare agent and terrorist threat since WWI. The cornea sensitive to SM toxicity exposure low vapor doses can cause incapacitating acute injuries. Exposure higher elicit persistent secondary keratopathies that reduced quality of life impaired or lost vision. Despite century research, there are no specific treatments for ocular cytotoxicity emerges, in part, through DNA alkylation double-strand breaks (DSBs). Because...
Transition metals are essential micronutrients for organisms but can be toxic to cells at high concentrations by competing with physiological in proteins and generating redox stress. Pathological conditions that lead metal depletion or accumulation causal agents of different human diseases. Some examples include anemia, acrodermatitis enteropathica, Wilson's Menkes' It is therefore important able measure the levels transport transition biological samples sensitivity accuracy order facilitate...
ABSTRACT Trace elements such as copper (Cu), zinc (Zn), iron (Fe), and manganese (Mn) are enzyme cofactors second messengers in cell signaling. emerging key regulators of differentiation development mammalian tissues including blood, brain, skeletal muscle. We previously reported an influx Cu dynamic expression various metal transporters during muscle cells. Here, we demonstrate that myoblasts increase additional trace Mn, Fe Zn occurs. Interestingly the Mn is concomitant with increased...
Transition metals are essential micronutrients for organisms but can be toxic to cells at high concentrations by competing with physiological in proteins and generating redox stress. Pathological conditions that lead metal depletion or accumulation causal agents of different human diseases. Some examples include anemia, acrodermatitis enteropathica, Wilson's Menkes' It is therefore important able measure the levels transport transition biological samples sensitivity accuracy order facilitate...
Abstract DNA G-quadruplexes (G4s) are secondary structures with significant roles in regulating genome function and stability. Dysregulation of the dynamic formation G4s is linked to genomic instability disease, but underlying mechanisms not fully understood. In this study, we conducted a screen chromatin-modifying enzymes identified nine potential inhibitors G4 formation, including seven that were previously characterized. Among these, highlight role BAZ2 chromatin remodelers as key...
ABSTRACT Understanding the molecular pathogenesis of MLL fusion oncoprotein (MLL-FP) leukaemia has spawned epigenetic therapies that have improved clinical outcomes in this often-incurable disease. Using genetic and pharmacological approaches, we define individual combined contribution KAT6A, KAT6B KAT7, MLL-FP leukaemia. Whilst inhibition KAT6A/B is efficacious some pre-clinical models, simultaneous targeting with novel inhibitor PF-9363, increases therapeutic efficacy. KAT7 interacts Menin...
Abstract MTF1 is a conserved metal-binding transcription factor in eukaryotes that binds to DNA sequence motifs, termed metal response elements (MREs). responds excess and deprivation, protects cells from oxidative hypoxic stresses, required for embryonic development vertebrates. We used multiple strategies identify an unappreciated role copper (Cu) cell differentiation. Upon initiation of myogenesis primary myoblasts, expression increased, as did nuclear localization. Mtf1 knockdown...
Transition metals like manganese (Mn) are necessary catalytic and structural cofactors in enzymes as second messengers cell signaling. Studies on the molecular mechanisms by which Mn contributes to development focused its role co‐factor of mitochondrial enzyme superoxide dismutase 2 (SOD2). However, has been proposed participate protein glycosylation during bone neural development. Our laboratory is investigating biological relevance differentiation skeletal muscle cells. During myogenesis,...