A5013069604- Malaria Research and Control
- Insect Resistance and Genetics
- Mosquito-borne diseases and control
- Evolution and Genetic Dynamics
- Genetic diversity and population structure
- Genetic and phenotypic traits in livestock
- CRISPR and Genetic Engineering
- Physiological and biochemical adaptations
- Insect symbiosis and bacterial influences
- Genetic Mapping and Diversity in Plants and Animals
- Genetic Associations and Epidemiology
- Insect Pest Control Strategies
- Parasite Biology and Host Interactions
- Neurobiology and Insect Physiology Research
- Genomic variations and chromosomal abnormalities
Imperial College London
2015-2024
University of London
2016
Abstract Background Measures of linkage disequilibrium (LD) play a key role in wide range applications from disease association to demographic history estimation. The true population LD cannot be measured directly and instead can only inferred genetic samples, which are unavoidably subject measurement error. Previous studies r 2 (a measure LD), such as the bias due finite sample size its variance, were based on special case that population-wise is zero. These results generally do not hold...
CRISPR-based homing gene drives can be designed to disrupt essential genes whilst biasing their own inheritance, leading suppression of mosquito populations in the laboratory. This class relies on CRISPR-Cas9 cleavage a target sequence and copying ('homing') therein drive element from homologous chromosome. However, site mutations that are resistant yet maintain function expected strongly selected for. Targeting functionally constrained regions where not easily tolerated should lower...
Abstract Diapause, a form of dormancy to delay or halt the reproductive development during unfavourable seasons, has evolved in many insect species. One example is aestivation, an adult‐stage diapause enhancing malaria vectors' survival dry season (DS) and their re‐establishment next rainy (RS). This work develops novel genetic approach estimate number proportion individuals undergoing diapause, as well breeding sizes two using signals from temporal allele frequency dynamics. Our modelling...
Abstract The effective population size Ne is a key parameter in genetics and evolutionary biology, as it quantifies the expected distribution of changes allele frequency due to genetic drift. Several methods estimating have been described, most direct which uses frequencies measured at two or more time points. A new likelihood-based estimator NB^ for contemporary using temporal data developed this article. existing likelihood are computationally intensive unable handle case when underlying...
Intensive deployment of insecticide based malaria vector control tools resulted in the rapid evolution phenotypes resistant to these chemicals. Understanding this process at genomic level is important for successful interventions. Therefore, longitudinal sampling followed by whole genome sequencing (WGS) necessary understand how evolutionary processes evolve over time. This study investigated change genetic structure and resistance variants natural populations Anopheles gambiae time space...
Abstract Intensive deployment of insecticide based malaria vector control tools results in the rapid evolution phenotypes resistant to these chemicals. Understanding this process on genomic level is essential for successful interventions. Using whole genome sequencing data 1409 individual An. Gambiae s.l. collected from 2012 2017, we investigated change genetic structure and resistance variants natural populations over time space. The showed similar constant nucleotide diversity negative...
In many locations malaria is transmitted by more than one vector species. Some control interventions, in particular those using genetic approaches, are likely to be targeted against a single species or complex, at least initially, and it would therefore useful able predict the epidemiological impact of controlling when multiple present. To address this issue, classical Ross-McDonald model epidemiology expanded account for species, giving expressions equilibrium prevalence, sporozoite rates...
The contemporary effective population size Ne is important in many disciplines including genetics, conservation science and pest management. One of the most popular methods estimating this quantity uses temporal changes allele frequency due to genetic drift. A significant assumption existing independence among loci while constructing confidence intervals (CI), which restricts types species or data applicable methods. Although linkage does not bias point estimates, applying these linked can...
Abstract CRISPR-based homing gene drives can be designed to disrupt essential genes whilst biasing their own inheritance, leading suppression of mosquito populations in the laboratory. This class relies on CRISPR-Cas9 cleavage a target sequence and copying (‘homing’) therein drive element from homologous chromosome. However, site mutations that are resistant yet maintain function expected strongly selected for. Targeting functionally constrained regions where not easily tolerated should...
Diapause, a form of dormancy to delay or halt the reproductive development during unfavourable seasons, has evolved in many insect species. One example is aestivation, summer adult-stage diapause, enhancing malaria vectors’ survival dry season (DS) and their re-establishment next rainy (RS). This work develops novel genetic approach estimate number proportion individuals undergoing as well breeding sizes two using signals from temporal allele frequency dynamics. Using Anopheles coluzzii an...