A5001580260- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genetic factors in colorectal cancer
- Gastric Cancer Management and Outcomes
- Cancer Immunotherapy and Biomarkers
- Helicobacter pylori-related gastroenterology studies
- Pancreatitis Pathology and Treatment
- Esophageal Cancer Research and Treatment
- Pancreatic function and diabetes
- Colorectal Cancer Treatments and Studies
- Neuroendocrine Tumor Research Advances
- Gastrointestinal disorders and treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Lung Cancer Research Studies
- Radiomics and Machine Learning in Medical Imaging
- Cancer Cells and Metastasis
- Colorectal Cancer Screening and Detection
- Autophagy in Disease and Therapy
- HER2/EGFR in Cancer Research
- Advanced Breast Cancer Therapies
- Gastrointestinal Tumor Research and Treatment
- Liver Disease and Transplantation
- Liver Disease Diagnosis and Treatment
- Gastroesophageal reflux and treatments
Huntsman Cancer Institute
2012-2025
University of Utah
2015-2025
ARUP Laboratories (United States)
2015-2025
BioEnergetics (United States)
2024
Université Grenoble Alpes
2024
Inserm
2024
University of Utah Hospital
2012-2021
National Institutes of Health
2020
Wellcome Trust
2020
Howard Hughes Medical Institute
2020
The 2018 ASCO/CAP guidelines for HER2 testing breast cancer implemented the addition of immunohistochemistry (IHC) directed in situ hybridization (ISH) recount to resolve equivocal results. implementation an additional 2+ IHC-directed ISH adds complexity workflow unclear impact on A retrospective review all cases (groups 2, 3, and 4) that underwent ISH, since guidelines, which were finalized as either amplified or not amplified, was performed. group number final amplification status...
Pharmacological inhibition of KRAS>RAF>MEK1/2>ERK1/2 signaling has provided no clinical benefit to patients with pancreatic ductal adenocarcinoma (PDAC). Interestingly, combined MEK1/2 (with trametinib [T]) plus autophagy chloroquine [CQ] or hydroxychloroquine [HCQ]) demonstrated striking anti-tumor effects in preclinical models and a patient (Patient 1). However, not all respond the T/HCQ regimen, Patient 1 eventually developed resistant disease. Here we report that primary acquired...
Abstract Purpose: Pure pancreatic acinar cell carcinomas (PACC) are rare malignancies with no established treatment. PACC demonstrates significant genetic intertumoral heterogeneity multiple pathways involved, suggesting using targeted cancer therapeutics to treat this disease. We aggregated one of the largest datasets pure examine genomic variability and explore patient-specific therapeutic targets. Experimental Design: specimens (n = 51) underwent next-generation sequencing DNA 29) or...
The serine/threonine‐protein kinase B‐raf ( BRAF ) is an oncogene mutated in various neoplasms, including 5–15% of colorectal carcinomas. T1799A point mutation, responsible for a large majority these alterations, results amino acid substitution (V600E) causing the constitutive activation protein cascade. V600E MLH1 deficient tumors implicates somatic tumor‐only methylation promoter region instead germline mutation. also predicts poor prognosis microsatellite stable cancers and may be marker...
The US Food and Drug Administration's Final Rule on laboratory-developed tests was published May 6, 2024. objective of this article is to explain the existing in vitro diagnostic regulations context anatomic pathology. Rule, regulations, guidance documents, government publications, websites, news articles, publications were reviewed, with sources including Federal Register, Code Regulations, Code, statutory text, PubMed, Internet resources. Regulations applicable device classifications...
To investigate the correlation between extent of (percentage tumor cells) immunohistochemistry (IHC) staining and final human epidermal growth factor receptor 2 (HER2)-positive result in fluorescence situ hybridization (FISH) groups to 4 with equivocal (2+) IHC requiring second, blinded FISH evaluation. Breast cancer cases submitted for HER2 testing group results were included. Of 2548 that had performed, 1104 (43.3%) (76/182 [41.8%] 2, 94/161 [58.4%] 3, 934/2205 [42.4%] 4) IHC. After second...
Environmental enrichment (EE) replicates mind-body therapy by providing complex housing to laboratory animals improve their activity levels, behavior, and social interactions. Using a Tcf4Het/+ ApcMin/+-mediated model of colon tumorigenesis, we found that EE vastly improved the survival tumor-bearing animals, with differential effect on tumor load in male compared female animals. Analysis ApcMin/+ males showed drastically reduced expression circulating inflammatory cytokines induced nuclear...
To identify gene expression biomarkers and pathways targeted by sulindac erlotinib given in a chemoprevention trial with significant decrease duodenal polyp burden at 6 months (
Routine application of PD-L1 immunohistochemistry (IHC) in colorectal cancer (CRC) is limited due to lack standardized scoring criteria, antibody clones, and intratumoral staining heterogeneity. We assessed protein expression on full face CRC tissue sections applied two algorithms based the published clinical trials that support recent FDA approval for immune checkpoint inhibitors (ICPI) therapy non-small cell lung (NSCLC).PD-L1/CD274 IHC (Roche/Ventana, clone SP142) was performed...
Significance of cytomegalovirus (CMV) in inflammatory bowel disease (IBD) is unclear due to pathobiology, numerous CMV tests, and disparate treatment outcomes.Retrospective chart review was done on patients with positive qualitative tissue polymerase chain reaction (PCR) from 2005-2013 at a tertiary referral hospital. Frequency PCR+, hematoxylin eosin staining(HE)+, histopathology immunohistochemistry (IHC)+ assessed. IHC assessed sample PCR- tissues. Surgery rates were correlated testing...
Abstract Purpose: Intraductal papillary mucinous neoplasms (IPMN) are bona fide precursors to pancreatic ductal adenocarcinoma (PDAC). While genomic alterations during multistep IPMN progression have been well cataloged, the accompanying changes within tumor immune microenvironment (TIME) not comprehensively studied. Herein, we investigated TIME-related progression, using multiplex immunofluorescence (mIF) coupled with high-resolution image analyses. Experimental Design: Two sets of...
We determined whether a large, multianalyte panel of circulating biomarkers can improve detection early-stage pancreatic ductal adenocarcinoma (PDAC).
Abstract Background The prognosis for nonmetastatic, primary pancreatic squamous cell carcinoma (SCC) is thought to be poor compared with adenocarcinoma (AC); however, this based on limited data. Additionally, the optimal definitive treatment strategy nonmetastatic SCC unknown. Methods We analyzed patients cancer using National Cancer Database diagnosed from 2006 2014. Patients were according histology—only AC, adenosquamous (A‐SCC), and included. endpoint was overall survival (OS) time of...
Intraductal papillary mucinous neoplasms (IPMN) are cystic precursor lesions to pancreatic ductal adenocarcinoma (PDAC). IPMNs undergo multistep progression from low-grade (LG) high-grade (HG) dysplasia, culminating in invasive neoplasia. While patterns of IPMN have been analyzed using multiregion sequencing for somatic mutations, there is no integrated assessment molecular events, including copy-number alterations (CNA) and transcriptional changes that accompany progression. We performed...
Abstract Background Histologic features of idiopathic non-cirrhotic portal hypertension (INCPH) may overlap with those without INCPH. Recently, these have been recognized as part the larger spectrum porto-sinusoidal vascular disease (PSVD). We assessed interobserver agreement on histologic that are commonly associated INCPH and studied whether a provision relevant clinical history improves agreement. Methods The examined include lobular (such anisocytosis, nodular regeneration, sinusoidal...
The confounding effects of next-generation sequencing (NGS) noise on detection low frequency circulating tumor DNA (ctDNA) without a priori knowledge solid mutations has limited the applications cell-free (ccfDNA) in clinical oncology. Here, we use 118 gene panel and leverage ccfDNA technical replicates to eliminate NGS-associated errors while also enhancing ctDNA from pancreatic ductal adenocarcinomas (PDACs). Pre-operative were acquired 14 patients with PDAC (78.6% stage II-III)....
Diagnostic test accuracy (DTA) studies on fine-needle aspiration cytology (FNAC) often show considerable variability in diagnostic between study centers. Many factors affect the of FNAC. A complete description testing parameters would help make valid comparisons and determine causes performance variation. We investigated manner which conditions are specified FNAC DTA to most commonly frequency with they see whether there is significant reporting practice. identified 17 frequently reported...
Abstract Neuroendocrine tumors (NETs) of the small intestine undergo large chromosomal and methylation changes. The objective this study was to identify differences in NETs consider how differentially methylated genes may impact patient survival. Genome-wide copy number variation (CNV) from appendix were measured. Tumors divided into three molecular subtypes according CNV results: chromosome 18 loss (18LOH), Multiple CNV, No CNV. Comparison 18LOH with MultiCNV NoCNV identified 901 genes....
Context.— Quantitative imaging is a promising tool that gaining wide use across several areas of pathology. Although there has been increasing adoption morphologic and immunohistochemical analysis, the evaluation fluorescence in situ hybridization (FISH) on formalin-fixed, paraffin-embedded tissue limited because complexity lack practice guidelines. Objective.— To perform human epidermal growth factor receptor 2 (HER2) FISH validation breast carcinoma accordance with American Society...