Background.Candida haemulonii, a yeast species that often exhibits antifungal resistance, rarely causes human infection. During 2004–2006, unusual isolates with phenotypic similarity to C. haemulonii were recovered from 23 patients (8 fungemia and 15 chronic otitis media) in 5 hospitals Korea.

ABSTRACT Biofilm production has been implicated as a potential virulence factor of some Candida species responsible for catheter-related fungemia in patients receiving parenteral nutrition. We therefore compared clinical bloodstream isolates representing seven different to each other and those from anatomical sites the capacity form biofilms glucose-containing medium. Potential associations between characteristics were also analyzed. Isolates included following nonneutropenic patients: 101...

Emergence of Candida haemulonii and closely related species at five Korean hospitals has been recently described. We examined biofilm formation by these isolates assessed their genotypic relatedness pulsed-field gel electrophoresis (PFGE). This study is the first to show that all bloodstream pseudohaemulonii can form significant biofilms in glucose-containing medium. PFGE NotI-digested genomic DNA revealed C. recovered from seven patients two shared patterns, 15 a proposed new (Candida...

ABSTRACT We investigated the azole resistance mechanisms and clinical features of fluconazole-nonsusceptible (FNS) isolates Candida tropicalis recovered from Korean surveillance cultures in comparison with fluconazole-less-susceptible (FLS) isolates. Thirty-five C. , comprising 9 FNS (fluconazole MIC, 4 to 64 μg/ml), 12 FLS (MIC, 1 2 14 control 0.125 0.5 μg/ml) isolates, were assessed. CDR1 MDR1 ERG11 expression was quantified, UPC2 genes sequenced. Clinical 16 patients or bloodstream...

New yeasts in the Pichia guilliermondii clade were isolated from digestive tract of basidiocarp-feeding members seven families Coleoptera. A molecular phylogeny and unique traits placed eight isolates Candida fermentati three undescribed taxa genus Candida. The new species type strains are C. smithsonii (type strain NRRL Y-27642T), athensensis Y-27644T), elateridarum Y-27647T). Based on comparison small-and large-subunit rDNA sequences, form a statistically well-supported subclade with P....

The echinocandin susceptibilities of bloodstream Candida isolates growing in a biofilm was investigated. Within the therapeutic range concentrations each drug, caspofungin and micafungin were active against biofilms formed by albicans or C. glabrata but not those tropicalis parapsilosis.

We examined the changes in genotypes and azole susceptibilities among sequential bloodstream isolates of Candida glabrata during course fungemia relationship these to antifungal therapy. Forty-one were obtained from 15 patients (9 who received therapy 6 did not) over periods up 36 days. The analyzed using pulsed-field gel electrophoresis (PFGE) multilocus sequence typing (MLST) tested for susceptibility fluconazole, itraconazole, voriconazole. PFGE consisted electrophoretic karyotyping...

ABSTRACT Natural killer T (NKT) cells are known to play a protective role in the immune responses of mice against variety infectious pathogens. However, little is about detailed information NKT patients with Mycobacterium tuberculosis infection. The aims this study were examine cell levels and functions active M. infection, investigate relationships between clinical parameters, determine mechanism responsible for poor response α-galactosylceramide (α-GalCer). significantly lower peripheral...

ABSTRACT We evaluated three commercial colistin susceptibility testing methods using 213 bloodstream Acinetobacter isolates identified by gene sequencing. Compared to the agar dilution reference method, excellent categorical agreements (both 99.1%) were observed Vitek 2 and Etest, compared 87.3% (95.7% for baumannii 80.7% non- isolates) MicroScan.

ABSTRACT Multilocus sequence typing (MLST) has been successfully applied to the epidemiology of Candida albicans isolates not only within hospital setting but also in multiple locations nationwide. We performed MLST investigate genetic relatedness among bloodstream infection (BSI) C. recovered from 10 Korean hospitals over a 12-month period. The 156 yielded 112 unique diploid types (DSTs). While 95 DSTs were each derived single isolate, 17 shared by 61 (39.1%). Interestingly, 111 (71.1%)...

Use of Xpert MTB/RIF assay as a substitute for smear microscopy in routine clinical practice remains unexplored an intermediate-tuberculosis-burden setting.To compare the diagnostic performance and microscopy, based on sampling time location, correlation semiquantitative category with grade to culture positivity, compliance reporting defined standard time.Consecutive sputum samples collected from 2,952 suspected pulmonary tuberculosis patients over 3-year period were tested by Xpert, liquid...

Data on clinical isolates of Kodamaea (Pichia) ohmeri, an emerging fungal pathogen, are scarce. Over the past 5 years, we identified yeast from nine patients with fungemia as K. ohmeri by using API 20C system. Here, reanalyzed these first sequencing internal transcribed spacer 2 (ITS2) regions and then growing CHROMagar Candida medium subjecting them to pulsed-field gel electrophoresis (PFGE). Based their ITS2 sequences, six were confirmed while others haemulonii (n = 2) parapsilosis 1)....

The emerging fungal pathogens Candida haemulonii and pseudohaemulonii often show high-level resistance to amphotericin B (AMB). We compared the utilities of five antifungal susceptibility testing methods, i.e., Etest using Mueller-Hinton agar supplemented with glucose methylene blue (Etest-MH), RPMI (Etest-RPG), Vitek-2 yeast system, Clinical Laboratory Standards Institute (CLSI) European Committee on Antimicrobial Susceptibility Testing (EUCAST) broth microdilution for detection...

Hyun-Seung Lee, M.D., Jong Hee Shin, Min Ji Choi, M.S., Eun Jeong Won, Seung Jung Kee, Soo Hyun Kim, Myung-Geun and Soon-Pal Suh, M.D.. Ann Lab Med 2017;37:223-30. https://doi.org/10.3343/alm.2017.37.3.223

sPurpose: The incidence of Candida bloodstream infections (BSI) has increased over the past two decades.The rank order occurrence and susceptibility to antifungals various species causing BSI are important factors driving establishment empirical treatment protocols; however, very limited multi-institutional data available on isolates in Korea.Materials Methods: We investigated azole distribution 143 recovered from eight university hospitals a six-month period.Minimal inhibitory...

We applied the new clinical breakpoints (CBPs) of Clinical and Laboratory Standards Institute (CLSI) to a multicenter study determine antifungal susceptibility bloodstream infection (BSI) isolates Candida species in Korea, determined relationship between frequency antifungal-resistant BSI use at hospitals. Four hundred fifty were collected over 1-year period 2011 from nine The susceptibilities four agents using CLSI M27 broth microdilution method. By applying species-specific CBPs,...

Intestinal parasitic diseases occur worldwide and can cause diarrhea or gastroenteritis; however, their diagnosis is quite difficult, especially in low-endemism countries. We developed a multiplex real-time PCR assay for detection of eight intestinal parasites prospectively evaluated it patients with gastroenteritis. The targeted Cryptosporidium parvum, Giardia lamblia, Entamoeba histolytica, Blastocystis hominis, Dientamoeba fragilis, Clonorchis sinensis, Metagonimus yokogawai,...

// Min-Gu Kang 1, 2, * , Hyun-Woo Choi Jun Hyung Lee 1 Yong Hyun-Jung Jong-Hee Shin Soon-Pal Suh Michael Szardenings 4 Hye-Ran Kim 5 Myung-Geun 3 Departments of Laboratory Medicine, Chonnam National University Medical School and Hwasun Hospital, 322 Seoyang-ro, Hwasun-eup, Hwasun-gun, Jeollanam-do, South Korea 2 Brain 21 Plus Project, School, Gwangju, Environmental Health Center for Childhood Leukemia Cancer, Department Cell Therapy, Fraunhofer Institute Therapy Immunology, Leipzig, Germany...

ABSTRACT We examined microevolution in a series of Candida albicans strains isolated from patients with catheter-related candidemia. Sixty-one isolates (29 blood, 18 catheters, 10 urine, and 4 other sites) were obtained 15 who admitted to the same hospital over 3-year period. Isolates analyzed by using Southern hybridization C1 fragment Ca3 as probe (C1 fingerprinting) pulsed-field gel electrophoresis (PFGE). PFGE typing consisted electrophoretic karyotyping (EK) restriction endonuclease...

Secreted aspartic proteases (Sap), encoded by a family of 10 SAP genes, are key virulence determinants in Candida albicans. Although biofilm-associated bloodstream infections (BSIs) frequently caused C. albicans, gene expression albicans biofilms formed BSI isolates has not been evaluated. We compared the two SAP5 and SAP9, with those from other body sites. Sixty-three were analyzed, comprising 35 28 A denture-strip biofilm model was used, genes quantified real-time RT-PCR during planktonic...

Schizophyllum commune, a basidiomycetous fungus, rarely causes disease in humans. We report rare case of allergic fungal sinusitis caused by S. commune 14-yr-old girl. The patient presented with nasal obstruction and purulent discharge. Materials obtained during endoscopic surgery the frontal recess revealed mucin few hyphae. A potato dextrose agar (PDA) culture from yielded rapidly growing white woolly mold. Although no distinctive features including hyphae bearing spicules or clamp...

We investigated the species distribution and amphotericin B (AMB) susceptibility of Korean clinical Aspergillus isolates by using two Etests CLSI broth microdilution method.A total 136 obtained from 11 university hospitals were identified sequencing internal transcribed spacer (ITS) β-tubulin genomic regions. Minimal inhibitory concentrations (MICs) AMB determined in Mueller-Hinton agar (Etest-MH) RPMI (Etest-RPG), categorical agreement with method was assessed epidemiological cutoff...