A5073415853- Intraperitoneal and Appendiceal Malignancies
- Cancer Research and Treatments
- Appendicitis Diagnosis and Management
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Hernia repair and management
- Photodynamic Therapy Research Studies
- Radiopharmaceutical Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Hypoxia, and Metabolism
- Cancer, Stress, Anesthesia, and Immune Response
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Neuroendocrine Tumor Research Advances
- Abdominal Surgery and Complications
- Cancer Cells and Metastasis
- Peptidase Inhibition and Analysis
- Adrenal and Paraganglionic Tumors
- Layered Double Hydroxides Synthesis and Applications
- Renal cell carcinoma treatment
- Glycosylation and Glycoproteins Research
- Hepatocellular Carcinoma Treatment and Prognosis
Radboud University Medical Center
2018-2022
Radboud University Nijmegen
2018-2022
Dutch Colorectal Cancer Group
2021
Radboud Institute for Molecular Life Sciences
2018-2020
<h3>Importance</h3> To date, no randomized clinical trials have investigated perioperative systemic therapy relative to cytoreductive surgery and hyperthermic intraperitoneal chemotherapy (CRS-HIPEC) alone for resectable colorectal peritoneal metastases (CPM). <h3>Objective</h3> assess the feasibility safety of in patients with CPM response neoadjuvant treatment. <h3>Design, Setting, Participants</h3> An open-label, parallel-group phase 2 trial all 9 Dutch tertiary centers surgical treatment...
In patients with colorectal peritoneal metastases scheduled for cytoreductive surgery, accurate preoperative estimation of tumor burden and subsequent intraoperative detection all deposits remains challenging. this study (ClinicalTrials.gov NCT03699332) we describe the results a phase I clinical trial evaluating [111In]In-DOTA-labetuzumab-IRDye800CW, dual-labeled anti-carcinoembryonic antigen (anti-CEA) antibody conjugate that enables both imaging radioguidance fluorescence imaging. Primary...
Abstract Purpose: Intraoperative image guidance may aid in clinical decision-making during surgical treatment of colorectal cancer. We developed the dual-labeled carcinoembryonic antigen–targeting tracer, [111In]In-DTPA-SGM-101, for pre- and intraoperative imaging Subsequently, we investigated tracer preclinical biodistribution multimodal image-guided surgery studies, assessed feasibility on patient-derived cancer samples, paving way rapid translation. Experimental Design: SGM-101 was...
Abstract Purpose To evaluate whether PET/CT could be used to assess the extent of colorectal peritoneal metastases. Methods All patients who underwent a scan before CRS-HIPEC procedure between January 1, 2010 and December 31, 2013 were retrospectively included ( n = 35). Two nuclear medicine physicians (observer 1 observer 2) separately reviewed scans on intraperitoneal abnormalities. A simplified PCI was compare rPCI versus sPCI. Results Included had median age 60.6 years. Histology primary...
<b><i>Background:</i></b> Cytoreductive surgery and hyperthermic intraperitoneal chemotherapy (CRS-HIPEC) is associated with considerable postoperative morbidity, including ileus infectious complications. Perioperative care believed to be an important factor for the development treatment of morbidity. <b><i>Patients Methods:</i></b> Data on case-matched patients from a retrospective database 2 Dutch HIPEC centres was compared. Patient selection...
Abstract Background Selected patients with colorectal peritoneal metastases are treated cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC). The concentration of reflects the administered dose perfusate volume. aim this study was to calculate during HIPEC see whether related clinical outcomes. Methods An observational multicentre included consecutive who were CRS–HIPEC between 2010 2018 at three Dutch centres. Data retrieved from prospectively developed...
Image-guided surgery can aid in achieving complete tumor resection. The development and assessment of tumor-targeted imaging probes for near-infrared fluorescence image-guided relies mainly on preclinical models, but the translation to clinical use remains challenging. In current study, we introduce evaluate application a dual-labelled tumor-targeting antibody ex vivo incubation freshly resected human specimens assessed tumor-to-adjacent tissue ratio detectable signals. Immediately after...
Abstract Background In colorectal cancer, survival of patients is drastically reduced when complete resection hampered by involvement critical structures. Targeted photodynamic therapy (tPDT) a local and targeted which could play role in eradicating residual tumor cells after incomplete resection. Since carcinoembryonic antigen (CEA; CEACAM5) abundantly overexpressed it potential target for tPDT cancer. Methods To address the CEA-targeted PDT, we compared cancer cell lines with different...
Image-guided surgery may improve surgical outcome for colorectal cancer patients. Here, we evaluated the feasibility of a pretargeting strategy multimodal imaging in using an anti-carcinoembryonic antigen (CEA) x anti-histamine-succinyl-glycine (HSG) bispecific antibody (TF2) conjunction with dual-labeled diHSG peptide (RDC018), both fluorophore near-infrared fluorescence and chelator radiolabeling. Nude mice subcutaneous (s.c) CEA-expressing LS174T human colonic tumors CEA-negative control...
Abstract Purpose In this exploratory study, the effect of postprocedural flushing with crystalloids after oxaliplatin-based hyperthermic intraperitoneal chemotherapy (HIPEC) on platinum concentrations in peritoneal tissue, blood, and drain fluid was studied. Interpatient variability oxaliplatin pharmacokinetics relation between concentration exposure tissue blood explored. Methods Ten patients carcinomatosis colorectal origin were treated HIPEC including flushing, followed by ten without...
TPS4119 Background: Successful treatment of patients with colorectal peritoneal carcinomatosis highly depends on complete surgical tumor resection all tumor. Oncological outcomes can potentially be improved by intraoperative imaging using a tumor-targeting antibody conjugated to fluorophore and radiotracer. This enables preoperative radionuclide imaging, real-time fluorescence gamma detection. In this study we investigate the feasibility, accuracy safety CEA-targeted SPECT/CT in PC. Methods:...
<p>Supplemental figure 4 shows slide 3 of at a higher magnification (5x). Clear colocalization CEA-expressing tumor cells can be observed. From left to right: H&E staining, CEA immunohistochemistry, NIR Fluorescence.</p>
<p>Microscopic analysis of resected intraperitoneal tumors shows clear overlap CEA-expressing tumor cells, NIR-fluorescence and radiosignal. In the bottom right corner a region with normal non-CEA expressing cells clearly low fluorescence (A) H&E, B) CEA, C) NIR-Fluorescence, D) Autoradiography.</p>
<p>Absorption and emission of SGM-101 DTPA-SGM-101. Measurements shown from 500 to nanometer, in steps 5 nanometer.</p>
<p>Biodistribution in mice with subcutaneous LS174T tumors at 24, 48 and 72 hours after injection of 30 μg [111In]In-DTPA-SGM-101.</p>
<div>AbstractPurpose:<p>Intraoperative image guidance may aid in clinical decision-making during surgical treatment of colorectal cancer. We developed the dual-labeled carcinoembryonic antigen–targeting tracer, [<sup>111</sup>In]In-DTPA-SGM-101, for pre- and intraoperative imaging Subsequently, we investigated tracer preclinical biodistribution multimodal image-guided surgery studies, assessed feasibility on patient-derived cancer samples, paving way rapid...
<div>AbstractPurpose:<p>Intraoperative image guidance may aid in clinical decision-making during surgical treatment of colorectal cancer. We developed the dual-labeled carcinoembryonic antigen–targeting tracer, [<sup>111</sup>In]In-DTPA-SGM-101, for pre- and intraoperative imaging Subsequently, we investigated tracer preclinical biodistribution multimodal image-guided surgery studies, assessed feasibility on patient-derived cancer samples, paving way rapid...
<p>Biodistribution in mice with subcutaneous LS174T tumors at 24, 48 and 72 hours after injection of 30 μg [111In]In-DTPA-SGM-101.</p>
<p>Supplemental figure 4 shows slide 3 of at a higher magnification (5x). Clear colocalization CEA-expressing tumor cells can be observed. From left to right: H&E staining, CEA immunohistochemistry, NIR Fluorescence.</p>
<p>Microscopic analysis of resected intraperitoneal tumors shows clear overlap CEA-expressing tumor cells, NIR-fluorescence and radiosignal. In the bottom right corner a region with normal non-CEA expressing cells clearly low fluorescence (A) H&E, B) CEA, C) NIR-Fluorescence, D) Autoradiography.</p>
<p>Absorption and emission of SGM-101 DTPA-SGM-101. Measurements shown from 500 to nanometer, in steps 5 nanometer.</p>
Additional file 1: Figure S2. Ex vivo biodistribution of two mice with subcutaneous LoVo tumors, one day after 111In-labeled DTPA-hMN-14-IRDye700DX injection.
Additional file 2: Figure S1. the CEA binding availability of cell lines used in this study. Note (arbitrary) classification high (>5% specific binding) and low (<5% DTPA-hMN-14-IRDye700DX.