A5067397754- Alzheimer's disease research and treatments
- Extracellular vesicles in disease
- Mitochondrial Function and Pathology
- Anesthesia and Neurotoxicity Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Glioma Diagnosis and Treatment
- Pharmacological Effects and Toxicity Studies
- Neuroscience and Neuropharmacology Research
- Advanced MRI Techniques and Applications
- Neurogenesis and neuroplasticity mechanisms
- Computational Drug Discovery Methods
University of Lausanne
2021-2025
University Hospital of Lausanne
2024
University Psychiatric Hospital
2024
Tauopathies are neurodegenerative disorders characterized by abnormal tau aggregation, with primary 3R (e.g., Picks disease, PiD) and 4R progressive supranuclear palsy, PSP) variants posing a significant diagnostic challenge. Here, we examined brain-derived extracellular vesicles (BD-EVs) isolated from the prefrontal cortex of PiD (3R), PSP (4R), non-demented controls (CTRL) to determine if these reflect disease-specific proteomic signatures. We found that while pathology does not...
Background: Synaptic dysfunction plays an important role in Alzheimer′s disease (AD) and is emerging imaging fluid biomarker. Here, we aimed to assess the regional expression of synaptic vesicle glycoprotein 2A (SV2A) brain extracellular vesicles AD patients its associations with APOE e4 allele, amyloid-&#61538, tau pathologies, other markers. Methods: Mass spectrometry-based synaptosome proteomics was performed on brain-derived (BdEVs) isolated from frontal cortex 17 4 NCs....
Tauopathies are neurodegenerative diseases characterized by tau inclusions in brain cells. Seed-competent species have been suggested to spread from cell a stereotypical manner, indicating that this may involve prion-like mechanism. Although the intercellular mechanisms of transfer unclear, extracellular vesicles (EVs) could be potential shuttles. We assessed humans preparing fluids (brain-derived enriched EVs [BD-EVs]). These latter were isolated different regions various tauopathies, and...
Tauopathies are neurodegenerative disorders involving the accumulation of tau isoforms in cell subpopulations such as astrocytes. The origins 3R and 4R that accumulate astrocytes remain unclear. Extracellular vesicles (EVs) were isolated from primary neurons overexpressing 1N3R or 1N4R human brain extracts (progressive supranuclear palsy Pick disease patients controls) characterized (electron microscopy, nanoparticle tracking analysis (NTA), proteomics). After EVs added to iPSC-derived...
Abstract Background Tauopathies encompass a spectrum of neurodegenerative disorders which are marked by the pathological aggregation tau protein into paired helical filaments (PHF-tau), neurofibrillary tangles (NFTs) and Glial-fibrillary (GFTs). These aggregates impair cellular, mitochondrial, synaptic functions. The emergence extracellular (ePHF-tau), featuring myriad isoforms phosphorylation states, presents challenge in comprehending its nuanced effects on neural cells, particularly...
<title>Abstract</title> <bold>Background</bold> Tauopathies encompass a spectrum of neurodegenerative disorders which are marked by the pathological aggregation tau protein into paired helical filaments (PHF-tau), neurofibrillary tangles (NFTs) and Glial-fibrillary (GFTs). These aggregates impair cellular, mitochondrial, synaptic functions. The emergence extracellular (ePHF-tau), featuring myriad isoforms phosphorylation states, presents challenge in comprehending its nuanced effects on...
In the dentate gyrus of hippocampus, neurogenic niche regulates several steps adult neurogenesis, from proliferation to integration newly formed neurons in hippocampal network. However, role astrocytes regulation neural stem cell (aNSC) is still little described. Here, we found that blocking vesicular release decreased gyrus, resulting impaired neurogenesis. Inversely, astrocyte-conditioned medium increased a release-dependent manner. We identified PEA116 as peptide released by astrocytes,...
Abstract Tauopathies are neurodegenerative disorders involving the accumulation of tau isoforms in cell subpopulations such as astrocytes. The origins 3R and 4R that accumulate astrocytes remain unclear. Extracellular vesicles (EVs) were isolated from primary neurons overexpressing 1N3R or 1N4R human brain extracts (progressive supranuclear palsy Pick disease patients controls) characterized (electron microscopy, nanoparticle tracking analysis (NTA), proteomics). After EVs added to...
While much attention has been given to mitochondrial alterations at the neuronal level, recent evidence demonstrates that dynamics and function in astrocytes are implicated cognition. This article describes method for time-lapse imaging of astrocyte cultures equipped with a biosensor: MitoTimer. MitoTimer is powerful unique tool assess dynamics, mobility, morphology, biogenesis, redox state. Here, different procedures culture, image acquisitions, subsequent analysis presented.
While much attention has been given to mitochondrial alterations at the neuronal level, recent evidence demonstrates that dynamics and function in astrocytes are implicated cognition. This article describes method for time-lapse imaging of astrocyte cultures equipped with a biosensor: MitoTimer. MitoTimer is powerful unique tool assess dynamics, mobility, morphology, biogenesis, redox state. Here, different procedures culture, image acquisitions, subsequent analysis presented.