It has been suggested that gut microbiota play a critical role in maternal metabolic oxidative stress responses and offspring growth. However, whether the status of sows affect litter performance during lactation is unclear. A total 66 Yorkshire were identified as high (H) or low (L) based on weight at day 21 lactation. Ten per group with similar parity, backfat thickness, after cross-foster from H L collected randomly to analyze The result showed serum antioxidant capacity was higher group,...

Intrauterine growth restriction (IUGR) is associated with fetal mortality and morbidity. One of the most common causes IUGR placental insufficiency, including vascular defects, mitochondrial dysfunction. In addition, a high level oxidative stress induces lesions. Here, we evaluated status, function, angiogenesis, nutrient transporters in placentae piglets different birth weights: <500 g (L), 500–600 (LM), 600–700 (M), >700 (H). Results showed that from L group had higher damage, lower...

Silage

Heat stress has a severe effect on animal health and can reduce the productivity reproductive efficiency; it is therefore necessary to explore molecular mechanism involved in heat response, which helpful for cultivation of an breed with resistance stress. However, little research about stress-responsive analysis been reported sheep. Therefore, this study, RNA sequencing (RNA-Seq) was used investigate transcriptome profiling liver Hu sheep without In total, we detected 520 22 differentially...

This study was conducted to investigate the effects of different additives on fermentation quality, nutrient composition, bacterial communities, and metabolic profiles silage hybrid Pennisetum. The experiment using five treatments, i.e., CK, control group, MA, 1% malic acid fresh matter (FM) basis, GL, glucose FM CE, 100 U/g cellulase, BS, 106 cfu/g Bacillus subtilis, with six replicates each treatment. After a 120-day fermentation, 30 packages were opened for subsequent determination. As...

The aim of this study was to investigate the effects dietary supplementation with a multi-strain probiotic (MSP) product containing Bifidobacterium animalis, Lactobacillus casei, Streptococcus faecalis, and Bacillus cerevisiae on growth, health, fecal bacterial composition dairy calves during first month life.

The present study investigated the effects of malic acid, sucrose, and their mixture on fermentation parameters, Cornell Net Carbohydrate Protein System (CNCPS) nitrogen fractions, bacterial community Moringa oleifera leaves (MOL) silages. trial was divided into four treatments labeled as CON (control group) MLA, SUC, MIX (respectively denoting addition 1% acid + sucrose to fresh weight basis). silage packages were opened 2nd, 5th, 10th, 20th, 40th days ensiling for subsequent determination....

The aim of this trial was to assess whether the supplementation vitamin E (VE) in high-concentrate diets could improve fermentation and blood metabolism rumen dairy cows, thereby modulating degree subacute ruminal acidosis (SARA) response improving performance. Seven Holstein cows (four fitted with cannulas) were fed three (total mixed rations) during successive periods (each lasted for 18 d): (1) control diet (CON); (2) a high-grain (HG) diet, which supplied 15% finely ground wheat (FGW);...

Litter size is an important indicator to measure the reproductive performance of goats, which affected by function animals. The hypothalamus, as regulatory center endocrine system, plays role in reproduction female Here, we performed high-throughput RNA sequencing using hypothalamic tissue from high-fecundity and low-fecundity Leizhou goats explore critical functional genes associated with litter size. Differentially expressed mRNA, lncRNA, circRNAs were screened DESeq enriched, then...

Capsaicin is the active ingredient of red pepper plant genus Capsicum. The aim this study was to investigate effects different doses capsaicin on growth performance, antioxidant capacity, immunity, fecal fermentation parameters and gut microbial composition in nursing calves. Twenty-four newborn Holstein calves were randomly assigned three treatment groups, which each consisted eight milk replacer supplemented with 0, 0.15 or 0.3 mL/d groups. During 4-week experiment, intake recorded daily,...

In this study, the effects of maternal energy sources during late gestation and lactation on performance, glucolipid metabolism, oxidative status sows their offspring were investigated using a total 75 (2 to 6 parity) Landrace × Large White at day 85 under 3 different dietary treatments: SO diet (basal plus 3.0% 5.0% soybean oil lactation, respectively), FO 3.0%/5.0% fish CS 32%/42% corn starch respectively). All groups showed no obvious differences (P > 0.05) in number piglets born, born...

This study investigated the effects of substitution whole corn silage (WCS) with Broussonetia papyrifera (BPS) in different ratios on serum indicators, hindgut fermentation parameters (pH, ammoniacal nitrogen, and volatile fatty acids), fecal bacterial community Holstein heifers. Sixteen heifers (8-month-old, 220 ± 30 kg) were randomly divided into four treatments according to BPS feed basis (0%, 25%, 50%, 75%). The experiment consisted a 7-day preliminary feeding period 30-day experimental...

In this study, changes in milk performance, nutrient digestibility, hindgut fermentation parameters and microflora were observed by inducing fat depression (MFD) dairy cows fed with a high-starch or high-fat diet. Eight Holstein paired completely randomized cross-over design within two 35 d periods (18 control period 17d induction period). During the period, all low-starch low-fat diet (CON), at four of crushed wheat (IS), other sunflower (IO). The results showed that, compared to when CON...

The proliferation and apoptosis of granulosa cells (GCs) affect follicle development reproductive disorders, with microRNAs playing a crucial regulatory role. Previous studies have shown the differential expression miR-128-3p at different stages goat development, which suggests its potential role in development. In this study, through Cell Counting Kit-8 assay, EDU flow cytometry, quantitative real-time polymerase chain reaction, Western blot, dual-luciferase reporter we used immortal human...

The progression of muscle development is a pivotal aspect animal ontogenesis, where miRNA and mRNA exert substantial influence as prominent players. It important to understand the molecular mechanisms involved in skeletal enhance quality yield meat produced by Leizhou goats. We employed RNA sequencing (RNA-SEQ) technology generate miRNA-mRNA profiles goats, capturing their developmental at 0, 3, 6 months age. A total 977 mRNAs 174 miRNAs were found be differentially expressed based on our...

This study investigates the effects of varying energy levels in diets on Black Angus steers, focusing growth performance, muscle composition, rumen microbial community, and their interrelationships. Twenty-seven aged approximately 22 months weighing 520 ± 40 kilograms, were randomly divided into three groups: low-energy (LE), medium-energy (ME), high-energy (HE). Each group consisted nine individuals. The steers fed with 6.657 MJ/kg 7.323 7.990 (HE) following a 14-day pre-feeding period,...

Litter size is a crucial quantitative trait in animals, closely linked to follicular development. Circular RNA (circRNA), type of single-stranded closed-loop endogenous with stable expression, plays pivotal roles various biological processes, yet its function goat development remains unclear. In this study, we collected large (follicle diameter > 3 mm) and small (1 mm < follicle follicles from black goats the Chuanzhong region for circRNA sequencing, aim elucidating functional circRNAs that...

Reproduction in goat is highly impeded by implantation failure. Of concern, the underlying mechanism leading to embryo remains unclear. In this study, deep sequencing was employed through strand-specific Ribo-Zero RNA-Seq characterize transcriptome changes endometrium during maternal recognition of pregnancy. A total 996 differential transcripts (115 lncRNAs and 881 mRNAs) existing between pregnant non-pregnant were revealed bioinformatics analysis. The screening performed on...

As an important organ that coordinates the neuroendocrine system, hypothalamus synthesizes and secretes reproductive hormones act on goat organism, thereby precisely regulating follicular development processes in goats. However, it is still elusive to explore mechanism of hypothalamic effects fertility alone. Therefore, RNA-seq was used analyze gene expression tissues goats high group (HFG: litter size per ≥2) low (LFG: = 1), identified differential lncRNAs mRNAs their associated pathways...

Dairy cow mastitis is one of the common diseases dairy cows, which will not only endanger health cows but also affect quality milk. an inflammatory reaction caused by pathogenic microorganisms and physical chemical factors in mammary glands. The number SCC milk with different degrees increase varying degrees. rapid diagnosis great significance for farm economy. Based on results many studies relationship between somatic cell count microflora, metabolites Holstein low level (SCC less than...

This study aimed to investigate the effects of oat hay feeding method and compound probiotics (CMP) on growth, health, serum antioxidant immune indicators, rumen fermentation, bacteria community dairy calves from 3 5 months age. Forty-eight female Holstein (80 ± 7 days age, 93.71 5.33 kg BW) were selected randomly divided into four groups. A 2 × factorial design was adopted for experiment, with factors (fed as free-choice or 16.7% in diet) inclusion (0.15% 0%) pelleted starter. The results...