A5002739087- Trypanosoma species research and implications
- Biochemical and Molecular Research
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Plant Virus Research Studies
- Research on Leishmaniasis Studies
- RNA Research and Splicing
- Adenosine and Purinergic Signaling
- Carbohydrate Chemistry and Synthesis
- CRISPR and Genetic Engineering
- Cardiomyopathy and Myosin Studies
- Seismic Imaging and Inversion Techniques
- CO2 Sequestration and Geologic Interactions
- PI3K/AKT/mTOR signaling in cancer
- Fungal Plant Pathogen Control
- Biotin and Related Studies
- Studies on Chitinases and Chitosanases
- Lysosomal Storage Disorders Research
- Methane Hydrates and Related Phenomena
University of Cambridge
2017-2025
Universidade de São Paulo
2018-2024
Fundação Oswaldo Cruz
2014
Ribosome assembly occurs mainly in the nucleolus, yet recent studies have revealed robust enrichment and translation of mRNAs encoding many ribosomal proteins (RPs) axons, far away from neuronal cell bodies. Here, we report a physical functional interaction between locally synthesized RPs ribosomes axon. We show that axonal RP is regulated through sequence motif, CUIC, forms an RNA-loop structure region immediately upstream initiation codon. Using imaging subcellular proteomics techniques,...
Selective transcription of individual protein coding genes does not occur in trypanosomes and the cellular copy number each mRNA must be determined post-transcriptionally. Here, we provide evidence that codon choice directs levels constitutively expressed mRNAs. First, a novel usage metric, gene expression adaptation index (geCAI), was developed maximised relationship between measured abundance for transcriptome. Second, geCAI predictions were tested using differently coded GFP transgenes...
The exon junction complex (EJC) is a key player in metazoan mRNA quality control and placed upstream of the exon-exon after splicing. Its inner core composed Magoh, Y14, eIF4AIII BTZ outer proteins involved splicing (CWC22), export (Yra1), translation (PYM) nonsense mediated decay (NMD, UPF1/2/3). Trypanosoma brucei encodes only two genes with introns, but all mRNAs are processed by trans -splicing. presence three EJC potential homologue (Rbp25) trypanosomes has been suggested to adapt...
Members of the eIF4E mRNA cap-binding family are involved in translation and modulation transcript availability other systems as part a three-component complex including eIF4G eIF4A. The kinetoplastids possess four described five homologs. We have identified two new proteins Trypanosoma brucei , define distinct complexes associated with fifth member, TbEIF4E5. cytosolic TbEIF4E5 protein binds cap 0 vitro. was found association TbEIF4Gs. TbIF4EG1 bound TbEIF4E5, 47.5-kDa RNA-binding domains,...
ABSTRACT Trypanosomes lack the transcriptional control characteristic of majority eukaryotes that is mediated by gene-specific promoters in a one-gene–one-promoter arrangement. Rather, their genomes are transcribed large polycistrons with no obvious functional linkage. Posttranscriptional regulation gene expression must thus play larger role these organisms. The eIF4E homolog TbEIF4E6 binds mRNA cap analogs vitro and part complex vivo may fulfill such role. Knockdown tagged protein A-tobacco...
Trypanosoma cruzi , the causing agent of Chagas disease, is only known trypanosomatid pathogenic to humans having a complete histidine glutamate pathway, which involves series four enzymatic reactions that convert into downstream metabolites, including urocanate, 4-imidazolone-5-propionate, N-formimino-L-glutamate and L-glutamate. Recent studies have highlighted importance this pathway in ATP production, redox balance, maintenance cellular homeostasis T. . In work, we focus on first step...
The fate of an mRNA is determined by its interaction with proteins and small RNAs within dynamic complexes called ribonucleoprotein (mRNPs). In Trypanosoma brucei related kinetoplastids, responses to internal external signals are mainly mediated post-transcriptional processes. Here, we used proximity-dependent biotin identification (BioID) combined RNA-seq investigate the changes resulting from ectopic expression RBP10 RBP9, two developmentally regulated RNA-binding (RBPs). Both RBPs have...
SUMMARY Ribosomes are known to be assembled in the nucleolus, yet recent studies have revealed robust enrichment and translation of mRNAs encoding ribosomal proteins (RPs) axons, far away from neuronal cell bodies. Using subcellular proteomics live-imaging, we show that locally synthesized RPs incorporate into axonal ribosomes a nucleolus-independent fashion. We RP is regulated through novel sequence motif, CUIC, forms RNA-loop structure region immediately upstream initiation codon....
Abstract Trypanosoma cruzi , the causative agent of Chagas disease, undergoes a complex life cycle involving triatomine insects as vectors and mammals. The differentiation epimastigote forms into metacyclic trypomastigotes within insect vector is crucial for parasite’s progression. Factors influencing this process, including temperature, pH, nutritional stress, along with specific metabolite availability, play pivotal role. Amino acids like Pro, His, Gln support cell differentiation, while...
Trypanosoma cruzi, the causative agent of Chagas disease, has a complex life cycle that involves triatomine insects as vectors and mammals hosts. The differentiation epimastigote forms into metacyclic trypomastigotes within insect vector is crucial for parasite's progression. Factors influencing this process, including temperature, pH, nutritional stress, along with specific metabolite availability, play pivotal role. Amino acids like proline, histidine, glutamine support cell...