A5044103960- Global Maternal and Child Health
- Healthcare Systems and Reforms
- Alzheimer's disease research and treatments
- Global Health Care Issues
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Fungal and yeast genetics research
- Cholinesterase and Neurodegenerative Diseases
- COVID-19 epidemiological studies
- Poverty, Education, and Child Welfare
- Global Public Health Policies and Epidemiology
- T-cell and B-cell Immunology
- Online and Blended Learning
- Vaccine Coverage and Hesitancy
- Cell death mechanisms and regulation
- SARS-CoV-2 and COVID-19 Research
- Neuroscience and Neuropharmacology Research
- Bacterial Genetics and Biotechnology
- Immune Cell Function and Interaction
- Pericarditis and Cardiac Tamponade
- Cardiac tumors and thrombi
- Monoclonal and Polyclonal Antibodies Research
- Marine and coastal ecosystems
- Fermentation and Sensory Analysis
- Agricultural risk and resilience
Asian Development Bank
2012-2024
SAIC-GM (China)
2024
Arrowhead Regional Medical Center
2022-2023
Asian Institute of Management
2012-2023
Riverside University Health System - Medical Center
2022
Neurological Surgery
2022
Asian Development Bank Institute
2018-2019
University of Missouri–Kansas City
2018
St. Luke's Hospital
2018
St. Vincent's Medical Center
2018
We generated a high-resolution whole-genome sequence and individually deleted 5100 genes in Sigma1278b, Saccharomyces cerevisiae strain closely related to reference S288c. Similar the variation between human individuals, Sigma1278b S288c average 3.2 single-nucleotide polymorphisms per kilobase. A genome-wide comparison of deletion mutant phenotypes identified subset that were conditionally essential by strain, including 44 unique 13 Genetic analysis indicates conditional phenotype was most...
The organization and sequences of the human beta-chain T-cell receptor diversity, joining, constant region segments are described. beta chain receptor, analogous to mouse counterpart, consists two distinct genes approximately equal 10 kilobases apart. genes, C 1 2, very similar not only in sequence but also genomic organization. coding each these divided into four exons. first exons encode most extracellular domain. third exon encodes a major part presumed transmembrane portion, last...
Background Mutuelles is a community-based health insurance program, established since 1999 by the Government of Rwanda as key component national strategy on providing universal care. The objective study was to evaluate impact achieving coverage medical services and financial risk protection in its first eight years implementation. Methods Findings We conducted quantitative evaluation between 2000 2008 using nationally-representative surveys. At provincial levels, we traced evolution child...
The dimorphic switch from a single-cell budding yeast to filamentous form enables Saccharomyces cerevisiae forage for nutrients and the opportunistic pathogen Candida albicans invade human tissues evade immune system. We constructed genome-wide set of targeted deletion alleles introduced them into S. strain, Σ1278b. identified genes involved in morphologically distinct forms filamentation: haploid invasive growth, biofilm formation, diploid pseudohyphal growth. Unique appear underlie each...
A cDNA clone representing the gene encoding beta chain of human T-cell antigen receptor has been isolated recently. By using fragments this as hybridization probes in Southern blot analysis restriction endonuclease-digested genomic DNA, we have now examined structure DNA from 26 patients with acute leukemia and 23 normal individuals. We found that undergone somatic rearrangement 14 phenotypic diagnosis lymphoblastic leukemia. In group patients, similar patterns appear to occur different...
We report the isolation and characterization of 19 classes nonrearranging T cell-specific cDNA clones two encoding alpha beta chains T-cell antigen receptor from a human line, Jurkat. Results indicate that alpha-chain gene, like its beta-chain counterpart, undergoes somatic rearrangement in cells. In addition, it shows sequence homology to counterpart immunoglobulin, indicating chain is also member immunoglobulin supergene family. Sequence comparison suggests may be composed variable (V),...
Abstract The unicellular green alga Chlamydomonas reinhardtii is a widely used model organism for studies of oxygenic photosynthesis in eukaryotes. Here we describe the development resource functional genomics using insertional mutagenesis nuclear genome. cells were transformed with either two plasmids conferring zeocin resistance, and mutants selected dark on acetate-containing medium to recover light-sensitive nonphotosynthetic mutants. population was subjected battery primary secondary...
ABSTRACT In an aerobic environment, responding to oxidative cues is critical for physiological adaptation (acclimation) changing environmental conditions. The unicellular alga Chlamydomonas reinhardtii was tested the ability acclimate specific forms of stress. Acclimation defined as a sublethal pretreatment with reactive oxygen species activate defense responses that subsequently enhance survival C. exhibited strong acclimation response rose bengal, photosensitizing dye produces singlet...
Objective To investigate the effect of survey design, specifically number items and recall period, on estimates household out-of-pocket catastrophic expenditure health.Methods We used results from two surveys -the World Health Survey Living Standards Measurement Study -that asked same respondents about health expenditures in different ways.Data were to compare average annual spending care derived a single-item an eight-item measure.This was done by calculating ratio obtained with measure...
When there is an imbalance between the light energy absorbed by a photosynthetic organism and that which can be utilized in photosynthesis, photo-oxidative stress damage pigments, proteins, lipids, nucleic acids. In this work we compared wild type xanthophyll-deficient mutant of Chlamydomonas reinhardtii their response to high amounts light. Wild-type cells were able acclimate following transfer from low conditions. contrast, npq1 lor1 double mutant, lacks protective xanthophylls (zeaxanthin...
Information and Communication Technologies (ICT) have had significant impacts on many sectors in today's knowledge economy. In developing emerging countries, ICT enhanced equity, quality, efficiency the education sector. However, adoption of South Asian countries' sector has not been at scale its limited. There are several gaps issues that hindering wider limiting Through case studies for Bangladesh Nepal, this paper examines to be addressed order better leverage enhance efficiency. This...
The relationship between treatment-related, cost-associated distress "financial toxicity" (FT) and quality-of life (QOL) in breast cancer patients remains poorly characterized. This study leverages validated patient-reported outcomes measures (PROMs) to analyze the association FT QOL satisfaction among women undergoing ablative surgery.This is a single-institution cross-sectional survey of all female (>18 years old) who underwent lumpectomy or mastectomy January 2018 June 2019. was measured...
Singlet oxygen is a highly toxic and inevitable byproduct of oxygenic photosynthesis. The unicellular green alga Chlamydomonas reinhardtii capable acclimating specifically to singlet stress, but the retrograde signaling pathway from chloroplast nucleus mediating this response unknown. Here we describe mutant, acclimation knocked-out 1 (sak1), that lacks oxygen. Analysis genome-wide changes in RNA abundance during revealed SAK1 key regulator gene expression acclimation. encodes an...
Information and Communication Technology (ICT) offers opportunities for governments to address key education challenges of quality, equity, efficiency. While educational institutions in developed countries may have taken up these opportunities, many developing Asia the Pacific region often missed them out. This be due countries' socioeconomic settings, approaches towards design implementation ICT plan, investment on research development education. paper explores current explains how overcome...
This paper attempts to isolate the causal link of income on health status and subjective well-being for rural population in Malawi using three waves household panel data spanning period 2004-2008 from Diffusion Ideational Chan
The connection between genotype and phenotype was assessed by determining the adhesion for same mutation in two closely related yeast strains, S288c Sigma, using identical deletion libraries. Previous studies, all had shown that controlled filamentation mitogen-activated kinase (fMAPK) pathway, which activates a set of transcription factors required structural gene FLO11. Unexpectedly, fMAPK pathway is not FLO11 despite fact genes are present active other pathways. Using transformation...
Caesarean sections (CS) is the most common lifesaving surgeries for obstructed labour and other emergency obstetrical conditions. The WHO had recommended ideal rate CS to be between 5% 15%. higher than 15% indicates overused lifesaving. Bangladesh has experienced a dramatic increase in delivery from 4% 2004 23% 2014. This elevated by several factors including maternal education, request or elective CS, urban richest population. However, little known about use poorest Therefore, study aimed...
Tau truncation (tr-tau) by active caspase-6 (aCasp-6) generates tau fragments that may be toxic. Yet the relationship between aCasp-6, different forms of tr-tau and hyperphosphorylated (p-tau) accumulation in human brains with Alzheimer's disease (AD) other tauopathies remains unclear.We generated two neoepitope monoclonal antibodies against sites (D402 D13) targeted aCasp-6. Then, we used five-plex immunofluorescence to quantify neuronal astroglial burden tr-tau, p-tau their co-occurrence...