A5089034223- Light effects on plants
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Greenhouse Technology and Climate Control
- Endoplasmic Reticulum Stress and Disease
- Photoreceptor and optogenetics research
- Protein Degradation and Inhibitors
- Photoacoustic and Ultrasonic Imaging
- Receptor Mechanisms and Signaling
- Endometriosis Research and Treatment
- Plant Growth Enhancement Techniques
- Silk-based biomaterials and applications
- Preterm Birth and Chorioamnionitis
- Tendon Structure and Treatment
- Advanced Fluorescence Microscopy Techniques
- Plant Gene Expression Analysis
- Plant Genetic and Mutation Studies
- Neurobiology and Insect Physiology Research
- Calcium signaling and nucleotide metabolism
- Reproductive System and Pregnancy
- Fungal and yeast genetics research
- Nanomaterials and Printing Technologies
- Plant-Microbe Interactions and Immunity
- Plant Physiology and Cultivation Studies
University of Mississippi
2020-2025
Chongqing Medical University
2025
Children's Hospital of Chongqing Medical University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
University of California, Riverside
2017-2023
Duke University
2014-2017
Washington State University
2011-2012
South China Agricultural University
1994
Ambient temperature sensing by phytochrome B (PHYB) in Arabidopsis is thought to operate mainly at night. Here we show that PHYB plays an equally critical role during the daytime. In daytime thermosensing, signals primarily through temperature-responsive transcriptional regulator PIF4, which requires activator HEMERA (HMR). HMR does not regulate PIF4 transcription, instead, it interacts directly with activate thermoresponsive growth-relevant genes and promote warm-temperature-dependent...
Abstract Warm temperature is postulated to induce plant thermomorphogenesis through a signaling mechanism similar shade, as both destabilize the active form of photoreceptor and thermosensor phytochrome B (phyB). At cellular level, shade antagonizes phyB by triggering disassembly from photobodies. Here we report temperature-dependent photobody localization fluorescent protein-tagged (phyB-FP) in epidermal cells Arabidopsis hypocotyl cotyledon. Our results demonstrate that warm elicits...
Plant phytochromes are thought to transduce light signals by mediating the degradation of phytochrome-interacting transcription factors (PIFs) through N-terminal photosensory module, while C-terminal including a histidine kinase-related domain (HKRD), does not participate in signaling. Here we show that module Arabidopsis phytochrome B (PHYB) is sufficient mediate PIF3 specifically and activate photosynthetic genes dark. The HKRD dimerization for PHYB homo heterodimerization. A D1040V...
Abstract Phytochromes (phys) are red and far-red photoreceptors that control plant development growth by promoting the proteolysis of a family antagonistically acting basic helix-loop-helix transcription factors, PHYTOCHROME-INTERACTING FACTORs (PIFs). We have previously shown degradation PIF1 PIF3 requires HEMERA (HMR). However, biochemical function HMR mechanism which it mediates PIF remain unclear. Here, we provide genetic evidence acts upstream PIFs in regulating hypocotyl growth....
MicroRNAs (miRNAs) play diverse roles in plant development, but whether and how miRNAs participate thermomorphogenesis remain ambiguous. Here we show that HYPONASTIC LEAVES 1 (HYL1)-a key component of miRNA biogenesis-acts downstream the thermal regulator PHYTOCHROME INTERACTING FACTOR 4 temperature-dependent plasticity hypocotyl growth Arabidopsis. A hyl1-2 suppressor screen identified a dominant dicer-like1 allele rescues hyl1-2's defects biogenesis thermoresponsive elongation. Genome-wide...
Calreticulin (CRT) is an endoplasmic reticulum-resident calcium-binding molecular chaperone that highly conserved in multi-cellular eukaryotes. Higher plants contain two distinct groups of CRTs: CRT1/CRT2 and CRT3 isoforms. Previous studies have shown bacterial elongation factor Tu receptor (EFR), a pattern-recognition responsible for pathogen-associated pattern-triggered immunity, substrate Arabidopsis CRT3, suggesting role regulating plant defense against pathogens. Here we report CRT2...
Abstract Daytime warm temperature elicits thermomorphogenesis in Arabidopsis by stabilizing the central thermoregulator PHYTOCHROME INTERACTING transcription FACTOR 4 (PIF4), whose degradation is otherwise promoted photoreceptor and thermosensor phytochrome B. PIF4 stabilization light requires a transcriptional activator, HEMERA (HMR), abrogated when HMR’s transactivation activity impaired hmr-22 . Here, we report identification of suppressor mutant, rcb-101 , which surprisingly carries an...
Temperature control is crucial for live cell imaging, particularly in studies involving plant responses to high ambient temperatures and thermal stress. This study presents the design, development, testing of two cost-effective heating devices tailored confocal microscopy applications: an aluminum heat plate a wireless mini-heater. The plate, engineered integrate seamlessly with standard 160 mm × 110 microscope stage, supports up 36°C, suitable range non-stressful warm (e.g., 25-27°C...
HEMERA (HMR) is a nuclear and plastidial dual-targeted protein. While it functions in the nucleus as transcriptional coactivator phytochrome signaling to regulate distinct set of light-responsive, growth-relevant genes, plastids known pTAC12, which associates with plastid-encoded RNA polymerase, essential for inducing plastomic photosynthetic genes initiating chloroplast biogenesis. However, mechanism targeting HMR still poorly understood. Here, we show that can be directly imported into...
Photoactivated phytochrome B (PHYB) binds to antagonistically acting PHYTOCHROME-INTERACTING transcription FACTORs (PIFs) regulate hundreds of light responsive genes in Arabidopsis by promoting PIF degradation. However, whether PHYB directly controls the transactivation activity PIFs remains ambiguous. Here we show that prototypic PIF, PIF3, possesses a p53-like activation domain (AD) consisting hydrophobic activator motif flanked acidic residues. A PIF3mAD mutant, which is replaced with...
Abstract While moderately elevated ambient temperatures do not trigger stress responses in plants, they substantially stimulate the growth of specific organs through a process known as thermomorphogenesis. The basic helix–loop–helix transcription factor PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) plays central role regulating thermomorphogenetic hypocotyl elongation various plant species, including Arabidopsis (Arabidopsis thaliana). Although it is well that PIF4 and its co-activator HEMERA...
Since its initial discovery as a high affinity Ca2+-binding protein in the sarcoplasmic reticulum and endoplasmic (ER), calreticulin (CRT) has been documented to be multifunctional both animal plant cells. This is well recognized molecular chaperone that facilitates folding of newly synthesized glycoproteins regulates Ca2+ homeostasis ER lumen. However, functional relevance associated with localization other cellular compartments also reported. Recent studies suggest isoforms CRTs (AtCRT1/2...
Microheaters have drawn extensive attention for their substantial applications in thermotherapy, gas sensors, thin film preparation, biological research, etc. In plant physiology, uncovering the mechanisms by which plants sense and respond to environmental temperature fluctuations will help us better understand impact of climate change on crop yield ecosystem resilience. Currently, microheaters with long-term heating capability rarely been applied investigate thermal responses. this study,...
Abstract Study Objective This study aimed to observe and quantitatively analyze the morphology distribution of uterine nerve fibers using fluorescence micro‐optical sectioning tomography (fMOST). The goal was provide an accurate morphological reference for pathological evaluations nerves. Measurements Main Results Using fMOST technique, we observed analyzed within uterus. Our findings revealed a radial dispersion radiating from myometrium endometrium. cervix uteri region exhibited high...