Toll-like receptor 9 (TLR9) recognizes non-methylated viral CpG-containing DNA and serves as a pattern recognition that signals the presence of human cytomegalovirus (HCMV). Here, we present genotype distribution single-nucleotide polymorphisms (SNPs) TLR9 gene in infants relationship between HCMV infection. Four (-1237T/C, rs5743836; -1486T/C, rs187084; 1174G/A, rs352139; 2848C/T, rs352140) were genotyped 72 with symptomatic infection 70 healthy individuals. SNP genotyping was performed by...

Intracellular Toll-like receptor 3 (TLR3) recognizes viral double-stranded RNA (dsRNA) and activates antiviral immune responses through the production of type I interferons (IFNs) inflammatory cytokines. This binds to dsRNA molecules produced during human cytomegalovirus (HCMV) replication. TLR7 senses single-stranded (ssRNA) in endosomes, it can interact with endogenous RNAs. We determined genotype distribution single-nucleotide polymorphisms (SNPs) within TLR3 genes children HCMV infection...

SUMMARY This study aimed to describe Toxoplasma gondii prevalence in Polish pregnant women and the incidence rates of congenital infections their neonates observed between 2004 2012. Serological tests for T. -specific IgG IgM antibodies were performed on serum samples 8281 treated at Mother's Memorial Hospital Research Institute Lodz. The yearly seroconversion rate was estimated using a mathematical model determine dependency age prevalence. Mean 2012 40·6% [95% confidence interval (CI)...

Background Some single nucleotide polymorphisms (SNP), located in Toll-like receptor (TLR) genes, were reported to be associated with human cytomegalovirus (HCMV) infections. The study was aimed assess the correlation of SNPs at TLR4 and TLR9 genes occurrence congenital cytomegaly, based on available samples. Methods Reported case-control included both HCMV infected non-infected fetuses newborns. specimens classified molecular analyses, serological features recent infection DNAemia body...

The purpose of this investigation was to perform an evaluation the prevalence and socioeconomic risk factors for human cytomegalovirus (HCMV) infections in a cohort Polish pregnant women between 2010 2011. HCMV-specific IgG IgM antibody levels were assayed with enzyme-linked immunosorbent assay (ELISA) tests serum samples collected from 1,250 attending outpatient obstetric clinics hospitalized at two hospitals Lodz. seroprevalence anti-HCMV antibodies 62.4 2.2 %, respectively, differed...

The purpose of this investigation was the determination distribution genotypes at single nucleotide polymorphisms (SNPs) toll-like receptor 4 (TLR4) and 9 (TLR9) in fetuses newborns congenitally infected with Toxoplasma gondii identification genetic changes predisposing to infection development. study involved 20 congenital toxoplasmosis 50 uninfected controls. levels IgG IgM antibodies against T. gondii, as well avidity, were estimated by enzyme-linked fluorescent assay (ELFA) tests. DNA...

Cytomegalovirus (CMV) is the most common cause of congenital infection. This pathogen exhibits extensive genetic variability in genes that encode structural envelope glycoproteins, regulatory proteins, and proteins contribute to immune evasion. However, role specific viral strains outcome CMV infection unclear. Variation UL55 gene encoding glycoprotein B (gB), UL144 TNF α-like receptor, US28 β-chemokine receptor was determined 60 newborn infants with 90 postnatal or undefined polymorphisms...

The purpose of this investigation was the determination distribution genotypes and alleles, residing within interleukin 6 (IL6) 1 (IL1) polymorphisms, among fetuses neonates, congenitally infected with Toxoplasma gondii, uninfected control cases. study included 22 newborns T. gondii 49 Screening for IgG IgM antibodies against parasite avidity performed by enzyme-linked fluorescent assay (ELFA) tests. Quantitation DNA in amniotic fluids assayed real-time Q PCR technique parasitic B1 gene....

Abstract Background Fibroblast growth factor 19 (FGF19), fibroblast 21 (FGF21) and Klotho are regulators of energy homeostasis. However, in the pediatric population, relationships between obesity, metabolic disorders aforementioned factors have not been clearly investigated. We analyzed role FGF19, FGF21 protein children with normal body weight as well overweight obese subjects explored their associations insulin resistance (IR) syndrome (MS) its components. Methods This was a...

The purpose of this investigation was to describe a distribution cytomegalovirus (CMV) single and multiple genotypes among infected pregnant women, their fetuses, newborns coming from Central Poland, as well congenital cytomegaly outcome. study involved 278 CMV-seropositive whom 192 were tested for viral DNAemia. Human (HCMV) genotyping performed 18 34 women carrying the DNA 12 15 offspring with confirmed HCMV infections. Anti-HCMV antibodies levels assessed by chemiluminescence immunoassay...

Preterm birth is defined as delivery before 37 completed weeks of pregnancy, and it the leading cause neonatal morbidity mortality. Oxidative stress recognized an important factor in pathogenesis premature labor. We conducted this analysis to investigate safety administration tocolytic drug Atosiban-a reversible, competitive antagonist oxytocin receptor treatment preterm its impact on level oxidative pregnant women after 48 hours treatment. This prospective study was between March 2016...

The objective of this study was to determine the concentration and reference ranges essential toxic elements in amniotic fluid (AF) maternal serum (MS) at birth. This conducted among 175 healthy pregnant Caucasian European women aged 18–42. AF blood samples were collected during delivery. An inductively coupled plasma mass spectrometry (ICP-MS) technique used levels Mg, Co, Cu, Zn, Sr, Cd, Ba, Pb, U, Ca, Cr, Al, Mn, V, Fe, As, Se Sb MS. range values calculated for all analyzed AF. mean...

Congenital birth defects may result in a critical condition affecting the baby, including severe fetal/neonatal handicap and mortality. Several studies have shown that genetic, nutritional, environmental factors an impact on fetal development neonatal health. The relevance of essential toxic elements has not yet been fully investigated, results recent research indicate these be crucial assessment risk malformations neonates. We determined association between level folate maternal serum (MS)...

Background The Matthew‐Wood syndrome is associated with mutations of the STRA6 gene. It combines a pulmonary agenesis/hypoplasia; microphthalmia/anophthalmia; congenital cardiac, digestive, and urogenital malformations; diaphragmatic defects. Case A 23‐year‐old nulliparous woman was referred to our center after fetal ultrasound examination at 26 weeks pregnancy revealed an abnormal head shape, heart malformation, multiple cysts in both kidneys, dilated ureters. male baby (46, XY; 3600g;...

Primary fetal chylothorax is an uncommon complication, associated with high perinatal morbidity and mortality. In our report, we describe two cases of bilateral primary successfully treated pleuro-amniotic shunting. both cases, ultrasound scans showed bilateral, hypoechoic fluid in the pleural space without any structural malformations features infection aneuploidy Laboratory analysis fluids revealed 79% 92% lymphocytes, respectively confirming fetuses. first case, shunts were inserted at 31...

Wrodzona cytomegalia wywołana transmisją wirusa cytomegalii (HCMV) od matki do płodu, jest najczęstszym zakażeniem wertykalnym