A5008233606- Pregnancy and preeclampsia studies
- Immune Cell Function and Interaction
- Prenatal Screening and Diagnostics
- Cardiovascular Issues in Pregnancy
- Assisted Reproductive Technology and Twin Pregnancy
- Maternal and Perinatal Health Interventions
- Mesenchymal stem cell research
- Birth, Development, and Health
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Cardiac Structural Anomalies and Repair
- Gestational Diabetes Research and Management
- Maternal and fetal healthcare
- Pregnancy and Medication Impact
- Pregnancy-related medical research
- Medical and Health Sciences Research
- Reproductive System and Pregnancy
- Neonatal Respiratory Health Research
- Pulmonary Hypertension Research and Treatments
- Virus-based gene therapy research
- Tissue Engineering and Regenerative Medicine
- Herpesvirus Infections and Treatments
- COVID-19 Impact on Reproduction
Medizinische Hochschule Hannover
2016-2025
Klinik für Frauenheilkunde
2009-2024
Marien Hospital Witten
2023
Beijing Obstetrics and Gynecology Hospital
2022
Deutsche Gesellschaft für Ultraschall in der Medizin
2020
Olgahospital
2020
Institute of Reproductive Medicine
2020
Arrowhead Regional Medical Center
2019
University of Alberta
2019
Makerere University
2019
Accumulating evidence suggests that the mouse embryonic thymus produces distinct waves of innate effector γδ T cells. However, it is unclear whether this process occurs similarly in humans and comprises a dedicated subset innate-like type 3 Here, we present protocol for high-throughput sequencing TRG TRD pairs comprise clonal γδTCR. In combination with single-cell RNA sequencing, multiparameter flow cytometry, TCR reveal high heterogeneity cells sorted from neonatal adult blood correlated...
Preterm infants are at high risk of developing neonatal sepsis. γδ T cells thought to be an important set effector in neonates. Here, were investigated a longitudinal cohort preterm neonates using next-generation sequencing, flow cytometry, and functional assays. During the first year life, Vγ9Vδ2 cell subset showed dynamic phenotypic changes elevated levels fetal-derived evident with Single-cell transcriptomics identified HLA-DRhiCD83+ sepsis, which expressed genes related antigen...
Abstract Mesenchymal stem (or stromal) cells (MSCs) have been used in more than 400 clinical trials for the treatment of various diseases. The benefit and reproducibility results, however, remain extremely variable. During vitro expansion phase, which is necessary to achieve clinically relevant cell numbers, MSCs show signs aging accompanied by different contributions single clones mass culture. Here we multicolor lentiviral barcode labeling follow clonal dynamics during MSC from whole...
First-trimester screening between 11 + 0 and 13 6 weeks with qualified prenatal counseling, detailed ultrasound, biochemical markers maternal factors has become the basis for decisions about further examinations. It detects numerous structural genetic anomalies. The inclusion of uterine artery Doppler PlGF screens preeclampsia fetal growth restriction. Low-dose aspirin significantly reduces prevalence severe preterm eclampsia. Cut-off values define groups high, intermediate low probability....
Epstein-Barr virus (EBV) is a latent and oncogenic human herpesvirus. Lytic viral protein expression plays an important role in EBV-associated malignancies. The EBV envelope glycoprotein 350 (gp350) expressed abundantly during lytic reactivation sporadically on the surface of latently infected cells. Here we tested T cells expressing gp350-specific chimeric antigen receptors (CARs) containing scFvs derived from two novel gp350-binding, highly neutralizing monoclonal antibodies. were fused to...
About 3% of newborns show malformations, with about 20% the affected having genetic causes. Clarification diseases in postnatal diagnostics was significantly improved high-throughput sequencing, particular through whole exome sequencing covering all protein-coding regions. Here, we aim to extend use this technology prenatal diagnostics.Between 07/2018 and 10/2020, 500 pregnancies fetal ultrasound abnormalities were analyzed after counseling as part using WES fetus parents.Molecular findings...
Before the turn of century, pregnancy in women with pulmonary arterial hypertension (PAH) was associated maternal mortality rates 30% and neonatal 11%.1Weiss BM Zemp L Seifert B Hess OM. Outcome vascular disease pregnancy: a systematic overview from 1978 through 1996.J Am Coll Cardiol. 1998; 31: 1650-1657Crossref PubMed Scopus (444) Google Scholar In more recent series, despite improved management PAH, pregnancy-associated remained high, ranging 11% to 25%.2Luo J Shi H Xu Su W Li J....
Neonates primarily rely on innate immune defense, yet their inflammatory responses are usually restricted compared to adults. This is controversially interpreted as a sign of immaturity or essential programming, increasing decreasing the risk sepsis, respectively. Here, combined transcriptomic, metabolic, and immunological studies in monocytes healthy individuals reveal an inverse ontogenetic shift metabolic pathway activities with age. Neonatal characterized by enhanced oxidative...
Abstract We have isolated a novel gene, LDOC1, which encodes for leucine zipper protein that was downregulated in series of human pancreatic cancer cell lines but expressed corresponding normal tissues. report the initial characterization LDOC1 as regulator transcriptional response mediated by nuclear factor kappa B (NF‐κB). Transient expression significantly inhibited luciferase activity LDOC1‐negative BxPC‐3 line transfected with NF‐κB reporter plasmid, activated mitogen‐activated...
Following allogeneic hematopoietic stem cell transplantation (HSCT), interferon (IFN)-gamma levels in the recipient's body can strongly influence clinical outcome. Human umbilical cord-derived mesenchymal cells (UC-MSCs) are lucrative as biological tolerance-inducers HSCT settings. Hence, we studied molecular mechanism of how UC-MSCs natural killer (NK) cell-mediated IFN-gamma production. Allogeneic NK were cultured direct contact with or free supernatants from MSC cultures (MSC conditioned...
Mesenchymal stem cells (MSCs) are increasingly considered to be used as biological immunosuppressants in hematopoietic cell transplantation (HSCT). In the early reconstitution phase following HSCT, natural killer (NK) represent major lymphocyte population peripheral blood and display graft-vs-leukemia (GvL) effects. The functional interactions between NK MSCs have potential influence leukemia relapse rate after HSCT. Until date, MSC-NK interaction studies largely focussed on bone marrow...
This extensive AWMF 085-002 S2e-guideline “First Trimester Diagnosis and Therapy @ 11–13+6 Weeks of Gestation” has systematically analyzed high-quality studies publications the existing evidence (evidence tables) produced recommendations (level recommendation, level evidence, strength consensus). guideline deals with following topics in context weeks scan: legal basis, screening for anatomical malformations, chromosomal defects, quality assessment audit, preeclampsia FGR, preterm birth,...
Abstract Background Mesenchymal stromal cells (MSCs) are used in over 800 clinical trials mainly due to their immune inhibitory activity. Umbilical cord (UC), the second leading source of clinically MSCs, is usually cut small tissue pieces. Subsequent cultivation leads a continuous outgrowth MSC explant monolayers (MSC-EMs) for months. Currently, first MSC-EM culture takes approximately 2 weeks grow out, which then expanded and applied patients. The initiating pieces discarded. However, when...
Abstract The following AWMF guideline (DGGG/AGG & DEGUM responsible) deals with the diagnosis, screening and management of twins as well timing mode birth. Twin pregnancies can be classified dichorionic diamniotic (DC DA), monochorionic (MC DA) monoamniotic MA) which are always monochorionic. concordant (both affected) or discordant (only one twin is for chromosomal defects, malformations, growth restriction hemodynamic disorders. Chorionicity prognostically most significant parameter....
Post-transplant lymphoproliferative disorder (PTLD) is one of the most common malignancies after solid organ or allogeneic stem cell transplantation. Most PTLD cases are B neoplasias carrying Epstein-Barr virus (EBV). A therapeutic approach reduction immunosuppression to allow T cells develop and combat EBV. If this not effective, approaches include immunotherapies such as monoclonal antibodies targeting CD20 adoptive cells. Immune checkpoint inhibition (ICI) treat EBV + was established...
Following the detection of fetal growth restriction, there is no consensus about criteria that should trigger delivery in late preterm period. The consequences inappropriate early or are potentially important yet practice varies widely around world, with abnormal findings from heart rate monitoring invariably leading to delivery. Indices derived cerebral Doppler examination may guide such decisions although few studies this area. We propose a randomised, controlled trial establish optimum...
Epigenetic mechanisms including DNA methylation are supposed to play a key role in fetal development. Here we have investigated DNA-methylation levels of 27,578 CpG loci 47 chorionic villi (CVS) and 16 amniotic cell (AC) samples. Methylation differed significantly between karyotypically normal AC CVS for 2,014 genes. showed more extreme these genes than the differentially methylated enriched processes characteristic different types sampled. Furthermore, identified 404 with trisomy 21. These...