A5065056332- Neonatal Respiratory Health Research
- Immune Response and Inflammation
- Influenza Virus Research Studies
- Respiratory viral infections research
- Immune Cell Function and Interaction
- Immune cells in cancer
- Immune responses and vaccinations
- Infant Nutrition and Health
- Pediatric health and respiratory diseases
- Gut microbiota and health
- T-cell and B-cell Immunology
- Clostridium difficile and Clostridium perfringens research
- Nuclear Receptors and Signaling
- Sepsis Diagnosis and Treatment
- CAR-T cell therapy research
- S100 Proteins and Annexins
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Viral gastroenteritis research and epidemiology
- Pregnancy and preeclampsia studies
- Child Nutrition and Water Access
- Electrolyte and hormonal disorders
- Immunotherapy and Immune Responses
- Birth, Development, and Health
Medizinische Hochschule Hannover
2019-2025
Universitätsklinikum Erlangen
2016
Background & AimsAfter birth, the immune system matures via interactions with microbes in gut. The S100 calcium binding proteins S100A8 and S100A9, their extracellular complex form, S100A8–A9, are found high amounts human breast milk. We studied levels of S100A8–A9 fecal samples (also called calprotectin) from newborns during infancy, effects on development intestinal microbiota mucosal system.MethodsWe collected stool (n = 517) full-term 72) preterm infants 49) at different timepoints over...
Significance T cell receptors (TCRs) on the surface of cells mediate recognition antigen. Since each new carries an individual clonal TCR, monitoring TCR repertoires reflects how react and proliferate in response to environmental cues developing immune system neonates children. γδ appear early during ontogeny are important for surveillance. Here, we longitudinally analyze show immediate polyclonal expansion phosphoantigen-reactive Vγ9Vδ2 + after birth. We also observed differences children...
Preterm infants are at high risk of developing neonatal sepsis. γδ T cells thought to be an important set effector in neonates. Here, were investigated a longitudinal cohort preterm neonates using next-generation sequencing, flow cytometry, and functional assays. During the first year life, Vγ9Vδ2 cell subset showed dynamic phenotypic changes elevated levels fetal-derived evident with Single-cell transcriptomics identified HLA-DRhiCD83+ sepsis, which expressed genes related antigen...
Neonates primarily rely on innate immune defense, yet their inflammatory responses are usually restricted compared to adults. This is controversially interpreted as a sign of immaturity or essential programming, increasing decreasing the risk sepsis, respectively. Here, combined transcriptomic, metabolic, and immunological studies in monocytes healthy individuals reveal an inverse ontogenetic shift metabolic pathway activities with age. Neonatal characterized by enhanced oxidative...
Introduction Respiratory viral infections (RVIs) are a major global contributor to morbidity and mortality. The susceptibility outcome of RVIs strongly age-dependent show considerable inter-population differences, pointing genetically and/or environmentally driven developmental variability. factors determining the age-dependency shaping age-related changes human anti-RVI immunity after birth still elusive. Methods We conducting prospective cohort study aiming at identifying endogenous...
Preterm birth is accompanied with many complications and requires severe therapeutic regimens at the neonatal intensive care unit. The influence of above-mentioned factors on premature-born infants' respiratory metagenome or more generally its maturation unknown. We therefore applied shotgun sequencing oropharyngeal swabs to analyze airway development 24 preterm infants from one week postpartum 15 months age. Beta diversity analysis revealed a distinct clustering microbial communities...
Newborn infants have a high disposition to develop systemic inflammatory response syndromes (SIRSs) upon or infectious challenges. Moreover, there is considerable trafficking of hematopoietic cells tissues already under noninflammatory conditions. These age-specific characteristics suggest hitherto unappreciated crucial role the vascular endothelium during neonatal period. Here, we demonstrate that healthy neonates showed strong endothelial baseline activation, which was mediated by...
Abstract Neonatal animal models are increasingly employed in order to unravel age-specific disease mechanisms. Appropriate tools objectifying the clinical condition of murine neonates lacking. In this study, we tested a scoring system specifically designed for newborn mice that relies on observation and examination. Both, neonatal sepsis model an endotoxic shock model, results strongly correlated with disease-induced death rates. Full as well observation-restricted scoring, reliably...
Abstract Protein-energy-malnutrition (PEM) is linked to 45% of global childhood mortality, however, the impact maternal PEM on child’s health remains elusive. Previous studies suggested that does not affect breast milk composition. Yet, malnourished children often develop a so-called environmental enteropathy assumed be triggered by frequent uptake pathogens together with unfavorable gut colonization. Here, we show in murine model induces without additional pathogen challenge offspring...
Abstract Respiratory viral infections (RVIs) are a major global contributor to morbidity and mortality. The susceptibility outcome of RVIs strongly age-dependent show considerable inter-population differences, pointing genetically and/or environmentally driven developmental variability. factors determining the age-dependency shaping age-related changes human anti-RVI immunity after birth still elusive. We conducting prospective cohort study aiming at identifying endogenous environmental...
Infections with influenza A viruses (IAV) cause seasonal epidemics and global pandemics. The majority of these infections remain asymptomatic, especially among children below five years age. Importantly, this is a time, when immunological imprinting takes place. Whether early-life IAV affect the development antimicrobial immunity unknown. Using preclinical mouse model, we demonstrate here that silent neonatal have remote beneficial impact on later control systemic juvenile-onset adult-onset...
TCR ligation is critical for the selection, activation, and integrin expression of T lymphocytes. Here, we explored role adaptor protein slp-76 on iNKT-cell biology. Compared to B6 controls, slp-76(ace/ace) mice carrying a missense mutation (Thr428Ile) within SH2-domain showed an increase in iNKT cells thymus lymph nodes, but decrease spleens livers, along with reduced ADAP cytokine response. A comparable reduction was observed livers ADAP-deficient mice. Like ADAP(-/-) cells, were...
SummaryAfter birth, the immune system experiences considerable reprogramming by interacting with colonizing microbiota. Host factors involved in this relationship are largely unknown. Our studies two infant cohorts and newborn mice reveal that abundant enteral S100A8/A9 trains inflammatory regulatory phenotype of colonic lamina propria macrophages. Neonatal S100A8/A9-alarmin deficiency results an impaired expression Cx3cr1, Il-10 Tgf-β, reduced expansion T cells, dominant gut colonization...