A5011540081- Immune Cell Function and Interaction
- Trace Elements in Health
- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- Viral Infections and Immunology Research
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Immunodeficiency and Autoimmune Disorders
- Whipple's Disease and Interleukins
- COVID-19 Clinical Research Studies
- Pregnancy and Medication Impact
- Platelet Disorders and Treatments
- Protein Degradation and Inhibitors
- Pneumocystis jirovecii pneumonia detection and treatment
- Eosinophilic Disorders and Syndromes
- Heavy Metal Exposure and Toxicity
- Heparin-Induced Thrombocytopenia and Thrombosis
- Peripheral Neuropathies and Disorders
- Airway Management and Intubation Techniques
- Aluminum toxicity and tolerance in plants and animals
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Tryptophan and brain disorders
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Peroxisome Proliferator-Activated Receptors
Otto-von-Guericke University Magdeburg
2015-2024
University Hospital Magdeburg
2015-2022
Dutch Research Council
1982
One-lung ventilation (OLV) results in alveolar proinflammatory effects, whereas their extent may depend on administration of anesthetic drugs. The current study evaluates the effects different volatile anesthetics compared with an intravenous and relationship between pulmonary systemic inflammation patients undergoing open thoracic surgery.Sixty-three scheduled for elective surgery were randomized to receive anesthesia 4 mg · kg⁻¹ h⁻¹ propofol (n = 21), 1 minimum concentration desflurane or...
Abstract Understanding the mechanisms leading to fetal death following maternal subclinical infections is crucial develop new therapeutic strategies. Here we addressed relevance of IL-10 secreting B cells (B10) in maintenance immune balance during gestation. µMT females lacking mature presented normal pregnancies, although their fetuses were smaller and Treg pool did not expand as cell sufficient controls. Pregnant more susceptible LPS despite having less Treg; died at doses compatible with...
Integrin activation is required for neutrophil functions. Impaired integrin on neutrophils the hallmark of leukocyte adhesion deficiency (LAD) syndrome in humans, characterized by impaired recruitment and recurrent infections. The Src kinase–associated phosphoprotein 2 (Skap2) involved functions different subtypes. However, role Skap2 β2 unknown. In this study, we demonstrate crucial regulating actin polymerization binding talin-1 kindlin-3 to cytoplasmic domain, thereby being indispensable...
Abstract : Dipeptidyl peptidase IV (DP IV, CD26) and DP IV-like enzymes, such as dipeptidyl II II), 8 (DP8), 9 (DP9), have been recognized to regulate T lymphocyte activation. Lys[Z(NO Here, we studied the influence of non-selective selective inhibitors enzymes on DNA synthesis in mitogen-stimulated splenocytes from wild-type C57BL/6 mice IV/CD26-knockout IV/CD26-KO) mice. LZNT LZNP, activity, suppressed stimulated IV/CD26-KO a comparable extent. Further, inhibitor DP8/DP9 activity was...
The COVID-19 pandemics has caused the death of almost six million people worldwide. In order to establish collective immunity, first vaccines that were approved in Germany vector virus-based vaccine Vaxzevria and mRNA Comirnaty Spikevax, respectively. As it was reported SARS-CoV-2 can trigger autoimmunity, is significant interest investigate whether evoke formation autoantibodies subsequent autoimmunity. Here, we analyzed immune responses after different vaccination regimens (mRNA/mRNA,...
Upon TCR stimulation by peptide-MHC complexes, CD4+ T cells undergo activation and proliferation. This process will ultimately culminate in T-cell differentiation the acquisition of effector functions. The production specific cytokines differentiated is crucial for generation appropriate immune response. Altered cytokine result chronic inflammatory conditions autoimmune disorders. miRNAs have been shown to be important regulators biology. In this study, we focused our investigation on...
The integrin LFA-1 (CD11a/CD18) plays a critical role in the interaction of T cells with antigen presenting (APCs) to promote lymphocyte differentiation and proliferation. This can be present either closed or an open active conformation its activation upon T-cell receptor (TCR) stimulation is step allow APCs. In this study we demonstrate that serine/threonine kinase Ndr2 critically involved initiation TCR-mediated (open conformation) cells. itself becomes activated TCR phosphorylates...
Abstract The genetic deletion of SKAP-HOM, an ubiquitously expressed cytosolic adapter protein, affected DC:T cell interactions reducing immune response. adaptor molecule similar to the T cell-specific homologue SKAP55, interacts directly with ADAP, and both molecules are involved in inside-out signaling. Previous studies have shown that absence antigen receptor-triggered integrin-mediated adhesion is impaired severely B cells but not cells. In addition, loss SKAP-HOM results a less severe...
Abstract The adhesion and degranulation promoting adaptor protein (ADAP) is a multifunctional scaffold involved in many different signaling pathways that are important for the function of T cells, including inside-out outside-in integrins, activation NF-κB, subsequent production proinflammatory cytokines (e.g., IFN-γ IL-2). Strikingly, despite its well-established role previous studies did not distinguish between CD4+ CD8+ thus, it unknown whether ADAP fulfills equally functions both cell...
After recovery, mild and severe COVID-19 diseases are associated with long-term effects on the host immune system, such as prolonged T-cell activation or accumulation of autoantibodies. In this study, we show that SARS-CoV-2 infections, but not spike mRNA vaccinations, cause durable atopic risk factors a systemic Th2- Th17-type environment well B cells responsive IgE against aeroallergens from house dust mite mold. At an average 100 days post anti-mold responses were low IL-13 levels...
The adhesion- and degranulation-promoting adaptor protein (ADAP), expressed in T cells, myeloid platelets, is known to regulate receptor-mediated inside-out signaling leading integrin activation adhesion. In this study, we demonstrate that, upon induction of active experimental autoimmune encephalomyelitis (EAE) by immunization with the myelin oligodendrocyte glycoprotein35-55 peptide, ADAP-deficient mice developed a significantly milder clinical course EAE showed markedly less inflammatory...
Statins are cholesterol reducers with considerable dose-dependent effect against glioma cells. The apoptotic could be increased by combining statins or adding pioglitazone (PGZ). last one is an anti-diabetic drug, agonist of the peroxisome proliferator-activated receptor-gamma (PPARG). We used animal model to test such combination in vivo and we investigated changes on immunological processes.Thirty-three rats (F344/DuCrl) were anesthetized glioblastoma (F98) cells implanted...
Abstract The TGF-β signaling pathways are implicated in cancer. Cysteine cathepsins can contribute to the carcinogenic potential of tumor cells. aim this study was investigate regulation cysteine cathepsin expression by TGF-β1 and functional implications We found an upregulation B (CathB, 2- 5-fold) different myeloid cells (THP-1, MonoMac-1, MonoMac-6) after incubation with TGF-β1. No monocytes, there suppression CathB epithelial (A549). Increased activity led enhanced potential, which...
Abstract T helper (Th) cells provide immunity to pathogens but also contribute detrimental immune responses during allergy and autoimmunity. Th2 mediate asthmatic airway inflammation Th1 are involved in the pathogenesis of multiple sclerosis. cell activation involves complex transcriptional networks metabolic reprogramming, which enable proliferation differentiation into cells. The essential trace element zinc has reported immunomodulatory capacity high concentrations interfere with...
Repositioning of approved drugs for identifying new therapeutic purposes is an alternative, time and cost saving strategy to classical drug development. Here, we screened a library 786 FDA-approved find compounds, which can potentially be repurposed treatment T cell-mediated autoimmune diseases. Investigating the effect these diverse substances on mitogen-stimulated proliferation both, freshly stimulated pre-activated (48 h) peripheral blood mononuclear cells (PBMCs), discovered Adefovir...
T cell activation mediates immunity to pathogens. On the flipside, cells are also involved in pathological immune responses during chronic autoimmune diseases. We recently reported that zinc aspartate, a registered drug with high bioavailability, dose-dependently inhibits and Th1/Th2/Th17 cytokine production of stimulated human mouse cells. To understand suppressive effect on function, we here investigated influence aspartate focusing secretion immunosuppressive cytokines, induction...