A5077313491- Gut microbiota and health
- Probiotics and Fermented Foods
- Nutritional Studies and Diet
- Diet and metabolism studies
- Dietary Effects on Health
- Cancer Immunotherapy and Biomarkers
- Gastrointestinal motility and disorders
- Infant Nutrition and Health
- Clostridium difficile and Clostridium perfringens research
- Nutrition and Health in Aging
- Mycobacterium research and diagnosis
- Adenosine and Purinergic Signaling
- Bacteriophages and microbial interactions
- Cancer Cells and Metastasis
- Biochemical Analysis and Sensing Techniques
- Phytoestrogen effects and research
- Colorectal Cancer Treatments and Studies
- Infant Health and Development
- Microbial Community Ecology and Physiology
- Antibiotic Resistance in Bacteria
- Gastric Cancer Management and Outcomes
- Colorectal Cancer Screening and Detection
- Neonatal Respiratory Health Research
- Phytochemicals and Antioxidant Activities
- Immune Cell Function and Interaction
University of Milan
2016-2025
Fondazione IRCCS Istituto Nazionale dei Tumori
2019-2023
Zero to Three
2016
Abstract Emerging evidence indicates that gut microbiota affect the response to anticancer therapies by modulating host immune system. In this study, we investigated impact of on immune-mediated trastuzumab antitumor efficacy in preclinical models HER2-positive breast cancer and 24 patients with primary undergoing trastuzumab-containing neoadjuvant treatment. mice, activity was impaired antibiotic administration or fecal transplantation from antibiotic-treated donors. Modulation intestinal...
Probiotics are exploited for adjuvant treatment in IBS, but reliable guidance selecting the appropriate probiotic to adopt different forms of IBS is lacking. We aimed identify markers recognizing non-constipated (NC) patients that may show significant clinical improvements upon with strain Lacticaseibacillus paracasei DG (LDG). To this purpose, we performed a post-hoc analysis samples collected during multicenter, double-blind, parallel-group, placebo-controlled trial which NC-IBS were...
Abstract Background Intestinal microbial composition not only affects the health of gut but also influences centrally mediated systems involved in mood, through “gut-brain” axis, a bidirectional communication between microbiota and brain. In this context, modulation intestinal its metabolites administration probiotics seems to represent very promising approach treatment central nervous system alterations. Early postnatal life is critical period during which brain undergoes profound essential...
The ATP-gated ionotropic P2X7 receptor regulates T follicular helper (Tfh) cell abundance in the Peyer's patches (PPs) of small intestine; deletion P2rx7, encoding for P2X7, Tfh cells results enhanced IgA secretion and binding to commensal bacteria. Here, we show that activity is important generating a diverse bacterial community gut sensing microbiota-derived extracellular ATP via promotes generation proficient ecosystem metabolic homeostasis. this study indicate play role host-microbiota...
Evidence suggests a role of intestinal microbiota-host interactions in the pathophysiology and symptoms irritable bowel syndrome (IBS).
Irritable bowel syndrome (IBS), a common functional gastrointestinal disorder, is classified according to habits as IBS with constipation (IBS-C), diarrhea (IBS-D), alternating and (IBS-M), unsubtyped (IBS-U). The mechanisms leading the different forms are mostly unknown. This study aims evaluate whether specific fecal bacterial taxa and/or short-chain fatty acids (SCFAs) can be used distinguish subtypes relevant for explaining clinical differences between subcategories. We characterized...
Fairly poor data are available on the relationship between taste perception, food preferences and oral microbiota. In present study, we investigated hypothesis that subjects with higher responsiveness to 6-n-propylthiuracil (PROP) might be characterized by a different sensitivity tongue microbiota composition. Indeed, bacterial metabolism may modulate/enhance concentration of tastants near receptors, modifying perception through sensorial adaptation mechanism or broad range microbial...
The role of taste perception, its relationship with oral microbiota composition, and their putative link eating habits food intake were the focus present study. A sample 59 reportedly healthy adults (27 male, 32 female; age: 23.3 ± 2.6 years) recruited for study thresholds basic tastes, intake, composition evaluated. Differences in perception associated different habitual consumption (i.e., frequency) actual intake. Subjects who orally hyposensitive to salty reported consuming more bakery...
ABSTRACT Modulation of the intestinal microbial ecosystem (IME) is a useful target to establish probiotic efficacy in healthy population. We conducted randomized, double-blind, crossover, and placebo-controlled intervention study determine impact Bifidobacterium bifidum strain Bb on IME adult volunteers both sexes. High-throughput 16S rRNA gene sequencing was used characterize fecal microbiota before after 4 weeks daily cell consumption. The intake approximately one billion live B. cells...
Microbial Electrochemical Technologies are based on the use of electrochemically active microorganisms that can carry out extracellular electron transfer to an electrode while they oxidizing organic compounds. The dynamics and changes bacterial community in anode biofilm planktonic broth acetate fed batch single chamber air cathode MFC have been studied by combing flow-cytometry Illumina sequencing techniques. At beginning test, from 0 h 70 h, microbial communities changed four groups two...
The MaPLE study was a randomized, controlled, crossover trial involving adults ≥60 y.o. (n = 51) living in residential care facility during an 8-week polyphenol-rich (PR)-diet. Results from the showed that PR-diet reduced intestinal permeability (IP) older by inducing changes to gut microbiota (GM). present work aimed at studying serum metabolome trial, as further necessary step depict complex crosstalk between dietary polyphenols, GM, and barrier.Serum monitored using semi-targeted...
Gut microbiota metabolization of dietary choline may promote atherosclerosis through trimethylamine (TMA), which is rapidly absorbed and converted in the liver to proatherogenic trimethylamine-N-oxide (TMAO). The aim this study was verify whether TMAO urinary levels be associated with fecal relative abundance specific bacterial taxa TMA-lyase gene cutC. analysis sequences available GenBank grouped cutC into two main clusters, cut-Dd cut-Kp. A quantitative real-time polymerase chain reaction...
Abstract Background During aging, alterations of the intestinal microbial ecosystem can occur contributing to immunosenescence, inflamm-aging and impairment barrier function (increased permeability; IP). In context a diet-microbiota-IP axis in older subjects, food bioactives such as polyphenols may play beneficial modulatory role. Methods MaPLE is project centered on randomized, controlled cross-over dietary intervention trial [polyphenol-rich diet (PR-diet) versus control (C-diet)] targeted...
Dietary polyphenols can alter the gut microbiota (GM) and promote production of bioactive metabolites. Several indoles result GM metabolism dietary tryptophan have been associated with intestinal barrier integrity. Our aim is to study changes in GM-derived during a polyphenol-rich (PR) diet intervention older adults.Randomized, controlled, crossover trial adults ≥ 60 years living residential care facility an 8-week PR versus control (n = 51). Seven GM-tryptophan metabolites are measured...
Several agri-food byproducts represent valuable sources of compounds to be reutilized for nutritional, nutraceutical, and cosmetic purposes. Examples especially comprise from the processing fruits such as pomace, because their richness in nutrients (e.g., fibers) bioactive polyphenols) that can destined animal human use. However, agreement with principles circular economy are being promoted during most recent years, other understudied both plant origin evaluated assess possible reutilization...
An involvement of gut microbiota on the possible role aspirin against intestinal adenoma (IA) and colorectal cancer (CRC) has been suggested. To further investigate this issue, we analyzed data from an Italian case-control study including 100 incident histologically confirmed CRC cases, as well IA controls without lesions colonoscopy, matched to cases by center, sex age. Serum zonulin was assessed ELISA kit blood bacterial DNA qPCR 16S rRNA gene profiling. Fifty-eight subjects (19.3%)...
The potential influence of insects' feeding behaviour on their associated bacterial communities is currently a matter debate. Using the major pest commodities, Plodia interpunctella, as model and adopting culture-independent approach, impact different diets host-associated microbiota was evaluated. An analysis similarity showed differences among microbiotas moths fed with five substrates provided evidence that diet represents only tested factor explains this dissimilarity. Bacteria shared...
Despite studies of viromes isolated from aquatic environments are becoming increasingly frequent, most them limited to the characterization viral taxonomy. Bacterial reads in abundant but extent which this genetic material is playing a role ecology microbiology remains unclear. To aim, we developed useful approach for and microbial communities with particular focus on identification genes harbored viromes. Virus-like particles were water samples collected across Lambro River, spring high...
Polyphenols have great potential in regulating intestinal health and ameliorating pathological conditions related to increased permeability (IP). However, the efficacy of dietary interventions with these phytochemicals may significantly be influenced by interindividual variability factors affecting their bioavailability consequent biological activity. In present study, urine samples collected from older subjects undergoing a crossover intervention trial polyphenol-rich foods were subjected...
Oral consumption of probiotics is practical and can be an effective solution to preserve vaginal eubiosis. Here, we studied the ability orally administered Lactobacillus paracasei LPC-S01 (DSM 26760) affect composition microbiota colonize mucosa in nondiseased adult women. A total 40 volunteers took oral probiotic (24 billion CFU) or placebo capsules daily for 4 weeks, after a 4-week washout, they switched according crossover design. 23 completed study protocol. Before capsule ingestion,...
Probiotic microorganisms may benefit the host by influencing diverse physiological processes, whose nature and underlying mechanisms are still largely unexplored. Animal models a unique tool to understand complexity of interactions between probiotic microorganisms, intestinal microbiota, host. In this regard, in pilot study, we compared effects 5-day administration three different bacterial strains ( Bifidobacterium bifidum MIMBb23sg, Lactobacillus helveticus MIMLh5, Lacticaseibacillus...
The mammary gland hosts a microbiota, which differs between malignant versus normal tissue. We found that aerosolized antibiotics decrease murine tumor growth and strongly limit lung metastasis. Oral absorbable also reduced tumors. In ampicillin-treated nodules, the immune microenvironment consisted of an M1 profile improved T cell/macrophage infiltration. these tumors, we noted under-representation microbial recognition complement pathways, supported by TLR2/TLR7 protein C3-fragment...
Garlic consumption has been inversely associated to intestinal adenoma (IA) and colorectal cancer (CRC) risk, although evidence is not consistent. Gut microbiota implied in CRC pathogenesis also influenced by garlic consumption. We analyzed whether dietary influence risk bacterial DNA blood.We conducted a case-control study Italy involving 100 incident cases, IA healthy controls matched center, sex age. used validated food frequency questionnaire assess habits Blood profile was estimated...