A5103071333- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Mesenchymal stem cell research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- HIV Research and Treatment
- Multiple Sclerosis Research Studies
- Herpesvirus Infections and Treatments
- Alzheimer's disease research and treatments
- Blood disorders and treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Peptidase Inhibition and Analysis
- Peripheral Neuropathies and Disorders
- Cholinesterase and Neurodegenerative Diseases
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- Neuropeptides and Animal Physiology
- Hematopoietic Stem Cell Transplantation
- Regulation of Appetite and Obesity
- Chronic Lymphocytic Leukemia Research
- Diabetes and associated disorders
- Immune Response and Inflammation
- Congenital Ear and Nasal Anomalies
- Parkinson's Disease Mechanisms and Treatments
Medizinische Hochschule Hannover
2014-2024
Center for Rheumatology
2013
Jeffrey Modell Foundation
2007
University of California, Los Angeles
1985-1989
UCLA Health
1989
Valley Hospital Medical Center
1989
University of California System
1986
The mesenchymal stroma harbors an important population of cells that possess stem cell-like characteristics including self renewal and differentiation capacities can be derived from a variety different sources. These multipotent (MSC) found in nearly all tissues are mostly located perivascular niches. MSC have migratory abilities secrete protective factors act as primary matrix for tissue regeneration during inflammation, injuries certain cancers.These functions underlie the physiological...
Primary immunodeficiencies (PIDs) represent exquisite models for studying mechanisms of human host defense. In this study, we report on two unrelated kindreds, with patients each, who had cryptosporidial infections associated chronic cholangitis and liver disease. Using exome candidate gene sequencing, identified distinct homozygous loss-of-function mutations in the interleukin-21 receptor (IL21R; c.G602T, p.Arg201Leu c.240_245delCTGCCA, p.C81_H82del). The IL-21RArg201Leu mutation causes...
Ocrelizumab, a humanized monoclonal anti-CD20 antibody, has shown pronounced effects in reduction of disease activity multiple sclerosis (MS) patients and recently been approved for the treatment with relapsing MS (RMS) primary progressive (PPMS). CD20 is mainly expressed by B cells, but subset T cells (CD3⁺CD20⁺ cells) also expresses CD20, these CD20⁺ are known to be highly activated cell population. The blood was analyzed multicolor flow cytometry before two weeks after ocrelizumab...
Leptin acts not only as an anorexigenic hormone but also regulates cell-mediated immunity via leptin receptors (Ob-R) expressed on T and B lymphocytes. However, the impact of natural killer (NK) cells is currently elusive. We evaluated effects NK in relation to body weight rats using vivo vitro approaches. was injected iv male lean diet-induced obese Lewis F344 rats. cell numbers were analyzed blood spleen by fluorescence activated sorting immunohistochemistry, activity measured chromium...
First isolated from bone marrow, mesenchymal stem or stromal cells (MSC) were shown to be present in several postnatal and extraembryonic tissues as well a large variety of fetal (e.g., fatty tissue, dental pulp, placenta, umbilical cord blood, tissue). In this study, an optimized protocol for the expansion MSC-like whole tissue under xeno-free culture conditions is proposed. Different calf sera human serum (HS) compared with regard cell proliferation MSC marker stability long-term...
NK cell function is important in the immune response to HIV infection. NKG2C and NKG2A are activating inhibitory receptors, respectively, their only known ligand, HLA-E, demonstrates increased expression infection presents at least one HIV-derived peptide. A variation chromosome 12 exists which 16-kb section of DNA encompassing nkg2c gene completely absent. samples 433 HIV-1-infected patients 280 controls were genotyped by PCR, revealed an association absence with a higher risk infection, as...
Anti-NMDA receptor encephalitis is the most common type of antibody mediated autoimmune (AIE). Patients often develop neuropsychiatric symptoms and seizures, women are affected about four times more than men, in 50% disease associated with a neoplasia, especially teratomas ovary. We describe case 20-year-old woman suffering from severe therapy refractory course anti-NMDA encephalitis. Treatment included glucocorticoids, plasma exchange, intravenous immunoglobulins, rituximab, bortezomib...
ABSTRACT: We have attempted to verify the presence of increased aluminum (Al) levels in Alzheimer's disease (AD) brains by energy dispersive X-ray microanalysis (EDX) and flameless atomic absorption spectrophotometry (AAS). Tissue from seven AD brains, mounted on carbon polymerized coverslips, were stained with Congored or treated immunohistochemically allow optical localization AD-associated lesions during EDX. Despite a demonstrated sensitivity 20-25 ppm, we unable detect Al either plaque...
Human cytomegalovirus (CMV) exerts diverse and complex effects on the immune system, not all of which have been attributed to viral genes. Acute CMV infection results in transient restrictions T cell proliferative ability, can impair control virus increase risk secondary infections patients with weakened or immature systems. In a search for new immunomodulatory proteins, we investigated UL11 protein, member RL11 family. This protein family is defined by domain, has homology immunoglobulin...
Following allogeneic hematopoietic stem cell transplantation (HSCT), interferon (IFN)-gamma levels in the recipient's body can strongly influence clinical outcome. Human umbilical cord-derived mesenchymal cells (UC-MSCs) are lucrative as biological tolerance-inducers HSCT settings. Hence, we studied molecular mechanism of how UC-MSCs natural killer (NK) cell-mediated IFN-gamma production. Allogeneic NK were cultured direct contact with or free supernatants from MSC cultures (MSC conditioned...
Mesenchymal stem cells (MSCs) are increasingly considered to be used as biological immunosuppressants in hematopoietic cell transplantation (HSCT). In the early reconstitution phase following HSCT, natural killer (NK) represent major lymphocyte population peripheral blood and display graft-vs-leukemia (GvL) effects. The functional interactions between NK MSCs have potential influence leukemia relapse rate after HSCT. Until date, MSC-NK interaction studies largely focussed on bone marrow...
Cytomegalovirus (CMV) is an opportunistic virus severely infecting immunocompromised individuals. In mice, endosomal Toll-like receptor 9 (TLR9) and downstream myeloid differentiation factor 88 (MyD88) are central to activating innate immune responses against mouse CMV (MCMV). this respect, the cell-specific contribution of these pathways in initiating anti-MCMV immunity remains unclear. Using transgenic we demonstrate that TLR9/MyD88 signaling selectively CD11c+ dendritic cells (DCs)...
Adult-onset primary immunodeficiency is characterized by recurrent infections, hypogammaglobulinemia and poor antibody response to vaccines. In this study, we have analyzed targeted gene panel sequencing results of 270 patients diagnosed with deficiency identified five disease causative variants in NFΚB1 unrelated families. We detected two single base pair deletions insertions, causing severe protein truncations one missense mutation. Immunoblotting, lymphocyte stimulation, immunophenotyping...
Abstract : Treatment of diabetes type 2 using chronic pharmacological inhibition dipeptidyl peptidase 4 (DP4) still requires an in-depth analysis models for DP4 deficiency, because adverse reactions induced by some inhibitors have been described. In the present study, a novel congenic rat model deficiency on “DP4-high” DA genetic background was generated (DA.F344- Similar to DP4, deficient rats exhibited phenotype involving reduced diet-induced body weight gain and improved glucose tolerance...
Parameters that regulate or affect the cell cycle DNA repair choice between non-homologous end-joining and homology-directed (HDR) are excellent targets to enhance therapeutic gene targeting. Here, we have evaluated impact of five cell-cycle modulating drugs on targeted genome engineering mediated by double-strand break (DSB)-inducing nucleases, such as zinc-finger nucleases (ZFNs). For a side-by-side comparison, established four reporter lines integrating mutated EGFP into either three...
Abstract Genetic and hygienic factors influence susceptibility to asthma. In autoimmune inflammatory diseases, additional effects of the psychosocial environment have been demonstrated that might also play a role in this study, impact different early postnatal stressors on an OVA-induced model asthma was tested adulthood. Fischer 344 rats were subjected either repeated handling stimulation (HA), maternal separation (MS), or left undisturbed their first 4 wk life. Behavioral differences...
In diet-induced obese rats, leptin-mediated natural killer (NK) cell activation has been demonstrated to be impaired by abrogated intracellular JAK2-STAT3 signaling. The contribution of the microenvironment this NK dysfunction and its reversibility remains elusive. study, functions cells from rats after adoptive transfer into lean littermates were investigated using in vivo vitro approaches. Endogenous normal-weight F344 depleted vivo. Then, either or donors transferred. numbers peripheral...