ABSTRACT The Campomanesia rufa is a fruitful species native from Brazil considered as endangered by the IUC (International Union for Conservation of Nature) and low information regarding its propagation available. In this context, aim present study was to develop in vitro germination, micropropagation callogenesis protocols species. For seeds were inoculated MS medium supplemented with GA3 (gibberellic acid) shoot induction, three different cytokinins BA (benzyladenine), BAP...

Abstract: Cryopreservation is a viable option for conservation of coffee germplasm. However, this technique to be completely successful, it fundamental importance carry out studies that ensure maintenance cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim study was investigate water content, cooling rate, final temperature most suitable cryopreservation Coffea arabica L. seeds. The seeds were dried by silica gel contents 5, 10, 15, 20, 30 40 % wb, subjected...

Annona glabra is a tropical species that has significant agronomic potential in terms of furnishing fruits for natura consumption and the production phyto-pharmaceuticals. In vitro cultivation been considered most promising form propagation this species, although large scale utilization technique currently limited by high rates leaf abscission, reduced explant multiplication slow bud growth. The present work evaluated effects different cytokinins on mineral accumulation shoots A. cultivated...

O objetivo desta pesquisa foi determinar um protocolo de micropropagação para o barbatimão. Para indução brotações, os segmentos nodais foram inoculados em meio cultura MS com diferentes concentrações cinetina (0, 1, 2, 3, 4 e 5mg L-1). raízes, brotações obtidas "in vitro" inicialmente transferidas contendo 50% da concentração sais ANA 3 4mg Posteriormente, as sais, suplementado carvão ativado. a aclimatização, plantas tubetes substratos e, então, envolvidas saco plástico transparente...

ABSTRACT Conservation of plant genetic resources is important to prevent erosion. Seed banks are the most common method ex situ conservation; however, coffee seeds can not be stored by conventional methods. Cryopreservation a viable alternative for long-term conservation species that produce intermediate or recalcitrant seeds, as coffee. The aim this work was cryopreserve Coffea arabica L. cv Catuaí Vermelho IAC 144 zygotic embryos, and analyse effects dehydration prior cryopreservation...

A micropropagação de anonáceas tem sido limitada pela abscisão foliar precoce nas brotações, o que dificulta a manutenção e desenvolvimento das plantas in vitro. Esse fato se deve, principalmente, ao acúmulo etileno nos tubos fechados à relação etileno/citocinina folhas. Assim sendo, avaliaram-se efeito fontes citocinina sobre retardo da senescência em brotações Annona glabra L. suas implicações seu desenvolvimento. Segmentos nodais foram cultivados tubo ensaio contendo 15 mL do meio WPM,...

Byrsonima intermedia A. Juss. é uma planta medicinal e frutífera do Cerrado, cuja propagação convencional difícil, devido à presença endocarpo extremamente lignificado. Embora o hipoclorito de sódio (NaOCl) seja amplamente utilizado para a desinfestação superficial, existem poucos relatos seus efeitos sobre crescimento explante. Neste trabalho, objetivo foi estudar efeito diferentes pHs períodos exposição sementes B. em solução NaOCl. As foram submetidas tempos (1, 5 10 minutos) numa NaOCl...

ABSTRACT Coffee is one of the main agricultural commodities in country, and it important to conservation plant material for breeding programs. Cryopreservation a promising alternative preserving long-term germplasm species considered recalcitrant. However, studies should be performed achieve maximum survival seedlings after immersion liquid nitrogen. The objective this work was find cryopreservation protocol storing seeds Coffea canephora, studying two methods cryopreservation, slow fast...

Fruits and almond from the dendezeiro, oil palmbelonging to Elaeis genus,are widely used for production of cookingoils or pharmaceutical cosmetic industries.In last decade, this palm also emerged as a promising source commercialbiofuel production. This study evaluated effect different culture media, MS (MURASHIGUE AND SKOOG) Y3 (EEUWENS)and carbohydrates duringin vitro germination zygotic embryos, growth regulators GA3, NAA BA Ponin seedling development, survival rate acclimatized...

O Inga vera Will subsp. affinis (DC). T.D. Penn. é uma espécie frutífera nativa do Cerrado, importante na recuperação de matas ciliares degradadas. Entretanto, apresenta sua propagação dificultada pelo fato suas sementes serem recalcitrantes, ou seja, não tolerarem a perda água. objetivo deste trabalho foi estudar aspectos da germinação ex vitro e in ingazeiro. Para tanto, foram avaliados os efeitos diferentes substratos: areia, Plantmax® Areia+ Plantmax®; concentrações sais: WPM, WPM/2, MS...

Byrsonima intermedia is a shrub from the Brazilian Cerrado with medicinal properties. The storage of biological material at ultra-low temperatures (-196°C) termed cryopreservation and represents promising technique for preserving plant diversity. Thawing crucial step that follows cryopreservation. aim this work was to cryopreserve B. zygotic embryos subsequently thaw them room temperature in solution rich sucrose. were decontaminated desiccated laminar airflow hood 0-4 hours prior plunging...

Hancornia speciosa Gomes is a fruit species belonging to the Apocynaceae family and holds social, cultural economic potential mostly due its use in composition of many food industry products consumption fruits natura.Several aspects regarding their propagation need further studies, since undergoing continuous process domestication.The objective was obtain an vitro protocol for indirect organogenesis rooting with subsequent acclimatization H. plants.To organogenesis, internodal segments were...

Seeds of the Coffea canephora species are considered more recalcitrant than those arabica species. They tolerate partial dehydration and they cannot be stored in conventional seed banks at -18ºC because sensitive to freezing temperatures. Cryopreservation is a reliable method for storing seeds with these characteristics long periods. However, preliminary studies necessary determine ideal storage conditions. The aim this study was investigate physical physiological conditions cryopreservation...

ABSTRACT Cryopreservation is a technique that may potentially conserve the germplasm of species Coffea genus for an indeterminate time. The aim this study was to evaluate physiological, biochemical and ultrastructural characteristics cryopreserved seeds arabica L., cultivar Catucaí amarelo IAC 62, which subjected different protocols regarding dehydration, precooling, cooling, rewarming cathode water use. According each protocol, were fast or slow drying moisture contents 17 20% (wet basis),...

Melocactus sergipensis, violaceus, and zehntneri are under threat of extinction. Their wild populations exploited by predatory extraction, because the potential as ornamental plants. Thus, alternatives for sustainable production conservation needed. The present study investigated influence artificial (growth room) natural light (greenhouse) on in vitro germination growth these three species, their effects during acclimatization. Plants were established cultivated 56 days two lighting...

The aim of this study was to evaluate the efficiency vitrification and droplet for cryopreservation Hancornia speciosa shoot tips.The tips were subjected four different periods exposure (15, 30, 45 60 min.) plant solution 2 (PVS2) before plunging into liquid nitrogen.We evaluated regrowth H. that cryopreserved by classical technique technique.Shoot submitted pre-culture (absence, 24 or 48h) in a medium containing 0.3 M sucrose prior cryopreservation.With PVS2 period min., significant...

ABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used propagate seed germination, but success limited by long time requirements and low germination percentage. An alternative for large-scale propagation biotechnological technique somatic embryogenesis. The rooting plants germinated embryos difficult step, yet it great later acclimatization in propagation. aim this study was evaluate effect auxins...

Melocactus zehntneri is a species of ornamental relevance suffering anthropogenic pressures, putting its preservation at risk. Thus, alternatives for propagating and conserving this are needed. This study evaluated the effect photoautotrophic cultivation M. to produce plants well acclimated natural conditions. The seeds underwent different imbibition times before in vitro germination. Besides growth room, greenhouse hosted seedlings. Vermiculite replaced agar culture medium. pre-treatment...

Brazil is one of the center origin passion fruit and has an important natural variability genus Passiflora. Several wild species this are resistant to some pests diseases many considered as medicinal. The aim research was induce callus from in vitro Passiflora gibertii leaf explants for quantification vitamin C total phenols. Once appropriate auxin/cytokine balance promotes formation may optimize production secondary compounds vitamins, calli were induced using a half-strength MS medium with...