RESUMO Agentes químicos produzidos em algumas plantas podem provocar alterações no desenvolvimento de outras ou até mesmo outros organismos. Ensaios laboratoriais demonstrar os resultados destas interações, porém, sem considerar a ação do ambiente. Com este trabalho objetivou-se identificar influência extratos vegetais 3 espécies conhecidas Brasil popularmente como boldo sobre o Allium cepa. Os bioensaios foram conduzidos germinador 25° C, com extrato aquoso obtido por infusão folhas frescas...

ABSTRACT Oil palm is one of the most economically valuable oil seed plants, but expansion plantations has been limited by availability seedlings, as conventional propagation through seeds, which have low germination rates. One possible solution for large-scale production use somatic embryogenesis. The aim this study was evaluate effects auxins 2,4-D and picloram on induction pro-embryogenic masses in E.guineenesis hybrid leaf explants characterize, regarding embryogenic characteristics, with...

Subcellular changes are relevant to understand plant organogenesis and embryogenesis in the early stages of cell development. The cytology during development tissue culture is however still poorly characterized. This study aimed characterize ultrastructural differences related callogenesis anthers, ovaries, leaf nodal segments Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Flower buds, leaves were disinfected inoculated test tubes containing MS medium with 3% sucrose 4.5µM 2.4-D, except...

Eucalyptus stands in the setting of worldwide forestry due to its adaptability, rapid growth, production high-quality and low cost wood pulp fibers. The eucalyptus convetional breeding is impaired mainlly by long life cycle making genetic transformation systems an important tool for this purpose. However, system requires vitro eficient protocols plant induction, regeneration seletion, that allow obtain transgenic plants from transformed cell groups. aim work was evaluate callus formation...

The plant micro-propagation in bioreactor systems is regarded as one way to reduce cost by automation and production scheduling. This research was carried out order obtain an efficient procedure for clone of Eucalyptus camaldulensis on different types including continuous temporary immersion bioreactor. To do so, the apical meristems (1 mm) with adjacent tissue (2,5 were used initial explants. These tissues cultured, 60 days, semisolid culture medium supplemented 1 mg L-1 indole acetic acid...

The objective of this work was to study the in vitro germination Passiflora gibertii N. E. Brown seeds, regarding scarification type, effect using GA3 growth regulator and use fresh or dry seeds. Ripe fruit seeds were washed water dried for four days (dry seeds). After period, new isolated from fruits (fresh Different methods tested (removing tip seed by forceps scalpel with sandpaper control treatment no scarification). scarification, inoculated MS culture medium, containing half its salt...

A mangabeira (Hancornia speciosa Gomes) destaca-se por possuir um grande potencial como planta frutífera e produtora de borracha. As dificuldades encontradas no seu processo propagação meio sementes, devido, principalmente, à baixa taxa germinação recalcitrância, valorizam a busca soluções alternativas para produção mudas dessa espécie, maneira rápida eficiente. Objetivou-se, neste trabalho, realizar o estudo da sementes em condições in vitro, tendo precedente obtenção explantes, posterior...

This work was carried out to characterise morphologically and ultrastructurally the banana callus, obtained from scalp method. Genotypes of banana, cv. Prata anã, cultivated in vitro were used induce meristems at leaf base; subsequently, structures known as scalps formed. For ultrastructural analysis, five samples callus collected, fixed Karnovsky solution analysed by scanning electron microscope (SEM) transmission (TEM). The formation three types observed: Type 1 - transparent watery 2...

The objective of this work was to elucidate the growth curve Eucalyptus camaldulensis Dehn. calli analyzing their anatomical modifications. A sigmoid aspect fresh matter observed, with five different phases (lag, exponential, linear, deceleration and decline). In lag phase, highest percentage 87%, which reduced during evaluation period 17% in linear phase. As for analyses, cellular multiplications observed exponential increase cell size promoting volume establishment globular conformation.

Fruits and almond from the dendezeiro, oil palmbelonging to Elaeis genus,are widely used for production of cookingoils or pharmaceutical cosmetic industries.In last decade, this palm also emerged as a promising source commercialbiofuel production. This study evaluated effect different culture media, MS (MURASHIGUE AND SKOOG) Y3 (EEUWENS)and carbohydrates duringin vitro germination zygotic embryos, growth regulators GA3, NAA BA Ponin seedling development, survival rate acclimatized...

The aim of this study was to induce and analyze embryogenic calli from two types explants (leaves meristems) the hybrid Eucalyptus grandis x urophylla. Leaves meristems plants kept in a nursery were disinfected inoculated Petri dishes containing MS culture medium supplemented with different concentrations growth regulator dicamba (1.13, 4.52, 9.04 µM) without it. At 60 days culturing, analyzed by scanning electron microscopy at 90 evaluated light regard characteristics cells. Different type...

This work studied a new protocol for organogenic calli induction and characterization of the morphology ultrastructure callogenesis in leaf explants Passiflora gibertii N. E. Brown, native passion fruit species from Brazil. Calli was performed different growth conditions (light dark), MS medium salt concentrations (MS half strength) presence or absence coconut water. The maintained dark were more responsive to bud formation. In order reduce spending on vitro culture, most suitable P....

O Inga vera Will subsp. affinis (DC). T.D. Penn. é uma espécie frutífera nativa do Cerrado, importante na recuperação de matas ciliares degradadas. Entretanto, apresenta sua propagação dificultada pelo fato suas sementes serem recalcitrantes, ou seja, não tolerarem a perda água. objetivo deste trabalho foi estudar aspectos da germinação ex vitro e in ingazeiro. Para tanto, foram avaliados os efeitos diferentes substratos: areia, Plantmax® Areia+ Plantmax®; concentrações sais: WPM, WPM/2, MS...

O objetivo deste trabalho foi descrever a curva de crescimento calos originados explantes foliares ingazeiro e observar o índice divisão celular durante curva.Os foram inoculadas em meio MS, 3% sacarose, 0,7% ágar 9 μM 2,4-D.Em intervalos 10 dias, os pesados.Para obtenção do mitótico, coletados 20 fixados Carnoy, posteriormente transferidos para 0,5 mL solução tampão McIlvaine, por minutos, peneirados centrifugados.O precipitado ressuspendido McIlvaine 20μL desta suspensão lâminas corados...

Campomanesia adamantium is a native plant species of Brazilian Cerrado with diverse economic potential and great medicinal importance. Its sexual propagation impaired by the recalcitrance its seeds, which prevents effective profitable propagation. With purpose establishing commercial crops minimizing extractive use vegetal resources, aim present study was to induce embryogenic calli in nodal segments gabirobeira, determine characterize their phase through establishment growth curve based on...

cell divisionÍndice mitótico e viabilidade celular de calos embriogênicos murici-pequenoThe Mitosis index and viability in embryonic calli of murici-pequeno Resumo: Byrsonima intermedia é uma espécie lenhosa medicinal ocorrência nos Cerrados brasileiros.O objetivo desta pesquisa foi determinar o padrão divisão células com características embriogênicas.Segmentos foliares foram inoculados em meio cultura MS suplementada 1,0 mg L -1 2,4-D.As repicagens efetuadas por três vezes, todas aos...