A5061622282- Environmental Toxicology and Ecotoxicology
- Toxic Organic Pollutants Impact
- Marine Bivalve and Aquaculture Studies
- MicroRNA in disease regulation
- Trace Elements in Health
- Effects and risks of endocrine disrupting chemicals
- Heavy Metal Exposure and Toxicity
- Epigenetics and DNA Methylation
- Heme Oxygenase-1 and Carbon Monoxide
- Calcium Carbonate Crystallization and Inhibition
- Heavy metals in environment
- Neonatal Health and Biochemistry
- Aquaculture disease management and microbiota
- Cancer, Hypoxia, and Metabolism
- Glutathione Transferases and Polymorphisms
- Genomics, phytochemicals, and oxidative stress
- Eicosanoids and Hypertension Pharmacology
- Animal Genetics and Reproduction
- Genomics and Phylogenetic Studies
- Zebrafish Biomedical Research Applications
- Aquaculture Nutrition and Growth
- Marine Biology and Environmental Chemistry
- Marine Toxins and Detection Methods
- Coral and Marine Ecosystems Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
University of Alabama
2011-2024
Woods Hole Oceanographic Institution
2006-2014
Innsbruck Medical University
1999-2012
NCCOS Hollings Marine Laboratory
2006-2007
Medical University of South Carolina
2002-2006
Universität Innsbruck
1999
Halogenated agonists for the aryl hydrocarbon receptor (AHR), such as 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), cause developmental toxicity in fish. AHR dependence of these effects is known TCDD but only presumed PCB126, AHR-regulated genes involved are part. We defined role regulation four cytochrome P450 1 (CYP1) effect PCB126 on cell cycle (i.e., PCNA cyclin E) zebra fish (Danio rerio) embryos. Basal PCB126-induced expression CYP1A, CYP1B1,...
Activation of the aryl hydrocarbon receptor (AHR) by 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin causes altered gene expression and toxicity. The AHR repressor (AHRR) inhibits signaling through a proposed mechanism involving competition with for dimerization nuclear translocator (ARNT) binding to AHR-responsive enhancer elements (AHREs). We sought delineate relative roles ARNT AHREs in repression. In transient transfections which AHR2-dependent transactivation was repressed AHRR1 or AHRR2,...
Abstract Background Populations of Atlantic killifish ( Fundulus heteroclitus ) have evolved resistance to the embryotoxic effects polychlorinated biphenyls (PCBs) and other halogenated nonhalogenated aromatic hydrocarbons that act through an aryl hydrocarbon receptor (AHR)-dependent signaling pathway. The is accompanied by reduced sensitivity induction cytochrome P450 1A (CYP1A), a widely used biomarker exposure effect, but whether specific CYP1A or reflects genome-wide reduction in...
Oxidative stress is an important mechanism of chemical toxicity, contributing to teratogenesis and cardiovascular neurodegenerative diseases. Developing animals may be especially sensitive chemicals causing oxidative stress. The developmental expression inducibility anti-oxidant defenses through activation NF-E2-related factor 2 (NRF2) affect susceptibility oxidants, but the embryonic response oxidants not well understood. To assess chemically mediated how it vary during development,...
Summary Organisms are continuously exposed to reactive chemicals capable of causing oxidative stress and cellular damage. Antioxidant enzymes, such as superoxide dismutases (SODs) catalases, present in both prokaryotes eukaryotes provide an important means neutralizing oxidants. Studies cnidarians have previously documented the occurrence antioxidant enzymes (transcript expression, protein and/or enzymatic activity), but most these studies not been conducted species with sequenced genomes or...
The aryl hydrocarbon receptor (AHR) repressor (AHRR), an AHR-related basic helix-loop-helix/Per-AHR nuclear translocator-Sim protein, is regulated by AHR-dependent mechanism and acts as a transcriptional of AHR function. Resulting from teleost-specific genome duplication, zebrafish have two AHRR genes (AHRRa AHRRb), but their functions in vivo are not well understood. We used antisense morpholino oligonucleotides (MOs) embryos liver cell line (ZF-L) to characterize the interaction AHRRs AHRs...
Freshwater mussels, aquatic keystone species, are in global decline. Long life spans, sedentary lifestyles, and unique reproductive strategies involving obligate parasitic stages make unionid freshwater mussels particularly sensitive to environmental perturbations resulting from climate change. A greater understanding of the mechanisms by which closely related species differ their response thermal challenge is critical for successful conservation management practices. As such, both an acute...
ABSTRACT Bumble bees are common in cooler climates and many species likely experience periodic exposure to very cold temperatures, but little is known about the temporal dynamics of response mechanisms following chill exposure, especially how persistent effects may facilitate tolerance future events. To investigate molecular processes involved by bumble acute we compared mRNA transcript abundance Bombus impatiens workers exposed 0°C for 75 min (inducing coma) control maintained at a constant...
Metallothioneins are typically low relative molecular mass (6000–7000), sulfhydryl‐rich metal‐binding proteins with characteristic repeating cysteine motifs (Cys‐X‐Cys or Cys‐X n ‐Cys) and a prolate ellipsoid shape containing single α‐ β‐domains. While functionally diverse, they play important roles in the homeostasis, detoxification stress response of metals. The originally reported metallothionein American oyster, Crassostrea virginica showed canonical molluscan αβ‐domain structure. Oyster...
The aryl hydrocarbon receptor (AHR) repressor (AHRR) inhibits AHR-mediated transcription and has been associated with reproductive dysfunction tumorigenesis in humans. Previous studies have characterized the function of AHRRs from mice fish, but human AHRR ortholog (AHRR(715)) appeared to be nonfunctional vitro. Here, we report a novel cDNA (AHRRDelta8) that lacks exon 8 AHRR(715). AHRRDelta8 was predominant form expressed tissues cell lines. effectively repressed AHR-dependent...
The aryl hydrocarbon receptor repressor (AHRR) is a transcriptional of (AHR) and hypoxia-inducible factor (HIF) regulated by an AHR-dependent mechanism. Zebrafish (Danio rerio) possess two AHRR paralogs; AHRRa regulates constitutive AHR signaling during development, whereas AHRRb polyaromatic hydrocarbon-induced gene expression. However, little known about the endogenous roles targets AHRRs. objective this study was to elucidate role AHRRs zebrafish development using loss-of-function...