A5019234030- Toxic Organic Pollutants Impact
- Environmental Toxicology and Ecotoxicology
- Effects and risks of endocrine disrupting chemicals
- Microplastics and Plastic Pollution
- Carcinogens and Genotoxicity Assessment
- Pharmacogenetics and Drug Metabolism
- Pharmaceutical and Antibiotic Environmental Impacts
- Zebrafish Biomedical Research Applications
- Mass Spectrometry Techniques and Applications
- Genomics, phytochemicals, and oxidative stress
- Marine Toxins and Detection Methods
- Mercury impact and mitigation studies
- Physiological and biochemical adaptations
- Cancer, Hypoxia, and Metabolism
- Glutathione Transferases and Polymorphisms
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Recycling and Waste Management Techniques
- Neuroscience and Neuropharmacology Research
- Animal testing and alternatives
- Marine animal studies overview
- Adipose Tissue and Metabolism
- Aquaculture disease management and microbiota
- Estrogen and related hormone effects
- Aquaculture Nutrition and Growth
Woods Hole Oceanographic Institution
2016-2025
Woodwell Climate Research Center
2015-2024
Bayer (Germany)
2024
Boston University
2000-2021
Environmental Protection Agency
2019
Oak Ridge Institute for Science and Education
2019
Northeastern University
2014
Wake Forest University
2014
University of Alberta
2008-2011
University of Ottawa
2008
We report the sequence and analysis of 814-megabase genome sea urchin Strongylocentrotus purpuratus , a model for developmental systems biology. The sequencing strategy combined whole-genome shotgun bacterial artificial chromosome (BAC) sequences. This use BAC clones, aided by pooling strategy, overcame difficulties associated with high heterozygosity genome. encodes about 23,300 genes, including many previously thought to be vertebrate innovations or known only outside deuterostomes....
The discovery of a living coelacanth specimen in 1938 was remarkable, as this lineage lobe-finned fish thought to have become extinct 70 million years ago. modern looks remarkably similar many its ancient relatives, and evolutionary proximity our own ancestors provides glimpse the that first walked on land. Here we report genome sequence African coelacanth, Latimeria chalumnae. Through phylogenomic analysis, conclude lungfish, not is closest relative tetrapods. Coelacanth protein-coding...
Atlantic killifish populations have rapidly adapted to normally lethal levels of pollution in four urban estuaries. Through analysis 384 whole genome sequences and comparative transcriptomics pairs sensitive tolerant populations, we identify the aryl hydrocarbon receptor-based signaling pathway as a shared target selection. This suggests evolutionary constraint on adaptive solutions complex toxicant mixtures at each site. However, distinct molecular variants apparently contribute...
Chronic pollution of the Hudson River, New York, results in rapid evolution resistance to pollutants.
The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor through which halogenated aromatic hydrocarbons such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) cause altered gene expression and toxicity. AHR belongs to the basic helix-loop-helix/Per-ARNT-Sim (bHLH-PAS) family of transcriptional regulatory proteins, whose members play key roles in development, circadian rhythmicity, environmental homeostasis; however, normal cellular function not yet known. As part...
The aryl hydrocarbon receptor (AHR) is a ligand-inducible transcription factor that best known because it mediates the actions of polycyclic and halogenated aromatic environmental toxicants such as 3-methylcholanthrene 2,3,7,8-tetrachlorodibenzo- p -dioxin. We report here successful identification an endogenous ligand for this receptor; ≈20 μg was isolated in pure form from 35 kg porcine lung. Its structure deduced 2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester extensive...
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and related halogenated aromatic hydrocarbons (HAHs) are highly toxic to most vertebrate animals, but there dramatic differences in sensitivity among species strains. Aquatic birds including the common tern (Sterna hirundo) exposed HAHs environment, up 250-fold less sensitive these compounds than typical avian model, domestic chicken (Gallus gallus). The mechanism of HAH toxicity involves altered gene expression subsequent activation aryl...
Halogenated agonists for the aryl hydrocarbon receptor (AHR), such as 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), cause developmental toxicity in fish. AHR dependence of these effects is known TCDD but only presumed PCB126, AHR-regulated genes involved are part. We defined role regulation four cytochrome P450 1 (CYP1) effect PCB126 on cell cycle (i.e., PCNA cyclin E) zebra fish (Danio rerio) embryos. Basal PCB126-induced expression CYP1A, CYP1B1,...
Activation of the aryl hydrocarbon receptor (AHR) by 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin causes altered gene expression and toxicity. The AHR repressor (AHRR) inhibits signaling through a proposed mechanism involving competition with for dimerization nuclear translocator (ARNT) binding to AHR-responsive enhancer elements (AHREs). We sought delineate relative roles ARNT AHREs in repression. In transient transfections which AHR2-dependent transactivation was repressed AHRR1 or AHRR2,...
Dioxin-like compounds are toxic to most vertebrates, but significant differences in sensitivity exist among species. A recent study suggests that the amino acid residues corresponding Ile324 and Ser380 chicken aryl hydrocarbon receptor 1 (AHR1) important determinants of differential biochemical responses 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) chickens common terns. Here, we investigate whether identity these can predict embryonic dioxin-like a wide range birds. AHR1 sequences were...
The aryl hydrocarbon receptor (AHR) binds to environmental toxicants including synthetic halogenated aromatic hydrocarbons and is involved in a diverse array of biological processes. Recently, the AHR was shown control host immunity by affecting balance between inflammatory T cells that produce IL-17 (Th17) IL-22 versus regulatory (Treg) tolerance. While ligands can mediate this effect, endogenous are likely be more relevant immune responses. We investigated downstream metabolites tryptophan...
The sensitivity of avian species to the toxic effects dioxin-like compounds (DLCs) varies up 1000-fold among species, and this variability has been associated with interspecies differences in aryl hydrocarbon receptor 1 ligand-binding domain (AHR1 LBD) sequence. We previously showed that LD50 values, based on ovo exposures DLCs, were significantly correlated vitro EC50 values obtained a luciferase reporter gene (LRG) assay measures AHR1-mediated induction cytochrome P4501A COS-7 cells...