A5030451379- Heat shock proteins research
- Endoplasmic Reticulum Stress and Disease
- Computational Drug Discovery Methods
- Ferrocene Chemistry and Applications
- X-ray Diffraction in Crystallography
- Toxin Mechanisms and Immunotoxins
- Crystallization and Solubility Studies
- Synthesis and Biological Evaluation
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Seaweed-derived Bioactive Compounds
- Marine Sponges and Natural Products
- Bioactive Compounds and Antitumor Agents
- Mass Spectrometry Techniques and Applications
- Cell Adhesion Molecules Research
- Microbial Metabolites in Food Biotechnology
- Viral Infectious Diseases and Gene Expression in Insects
- Insect Resistance and Genetics
- Metal complexes synthesis and properties
- Herpesvirus Infections and Treatments
- Adipose Tissue and Metabolism
- Cancer Cells and Metastasis
- Click Chemistry and Applications
- Peptidase Inhibition and Analysis
- Cellular Mechanics and Interactions
Rhodes University
2016-2025
University of Glasgow
2007-2012
St Thomas' Hospital
2005
The discovery of a living coelacanth specimen in 1938 was remarkable, as this lineage lobe-finned fish thought to have become extinct 70 million years ago. modern looks remarkably similar many its ancient relatives, and evolutionary proximity our own ancestors provides glimpse the that first walked on land. Here we report genome sequence African coelacanth, Latimeria chalumnae. Through phylogenomic analysis, conclude lungfish, not is closest relative tetrapods. Coelacanth protein-coding...
Many heat shock proteins (HSPs) are essential to survival as a consequence of their role molecular chaperones, and play critical in maintaining cellular proteostasis by integrating the fundamental processes protein folding degradation. HSPs arguably among most prominent classes that have been broadly linked many human disorders, with changes expression profile and/or intracellular/extracellular location now being described contributing pathogenesis number different diseases. Although concept...
The central role of the chaperome in maintaining cellular proteostasis has seen numerous viral families evolve to parasitically exploit host chaperones their life cycle. HSP90 chaperone protein and its co-chaperone Hop, have both individually been shown be essential factors for KSHV lytic replication. Given fundamental regulatory that PPIs play biology, we reasoned disrupting Hop-HSP90 PPI may provide a new host-based target inhibiting This study expands upon previous report non-natural...
Heat shock protein 90 (Hsp90) has been identified in the extracellular space and shown to chaperone a finite number of proteins involved cell migration invasion. We used chemical cross-linking immunoprecipitation followed by tandem mass spectrometry (MS/MS) isolate complex containing Hsp90 matrix fibronectin (FN) from breast cancer cells. Further analysis showed direct binding FN using an vitro co-immunoprecipitation assay, solid phase assay surface plasmon resonance (SPR) spectroscopy....
Deoxyribonucleic acid (DNA) and bovine serum albumin (BSA) binding interactions for a series of ruthenium heterocyclic complexes were monitored using ultraviolet-visible (UV-Vis) spectrophotometry, fluorescence emission spectroscopy agarose gel electrophoresis. Investigations the DNA metal revealed that they are groove-binders with intrinsic constants in order 104 – 107 M−1. Electronic spectrophotometric titrations bis-heterocyclic illustrated hypochromism their intraligand electronic...
Cadmium telluride (CdTe) semiconductor quantum dots (QDs) are brightly luminescent nanocrystals that have emerged as a new class of fluorescent probes for in vivo bioimaging and theranostic applications. CdTe QDs toxicity to normal human cells is minimized by coating with less toxic ZnS ZnSe shell forming core–shell nanostructure. However, or insufficient prevent the leaching Cd metal ions. To further minimize toxicity, thiol dual capped CdTe/CdSe/ZnSe multi-core-multi-shell were coated...
<title>Abstract</title> Semi-synthetic derivatives of artemisinin exhibit anti-cancer activity <italic>in vitro</italic> and vivo</italic> in addition to anti-malarial activity. Here, we report the stem cell potential novel C-10 substituted amino-artemisinin derivatives, among which 4'-trifluoromethylarylurea piperazinyl derivative WHN-11 demonstrated consistent cytotoxic at high nanomolar concentrations across a range cancer lines. reduced short- long-term survival triple-negative breast...
Obesity and type 2 diabetes mellitus (T2DM) are major health concerns worldwide, often managed with treatments that have significant limitations side effects. This study examines the potential of sodium alginates, extracted from Ecklonia radiata Sargassum elegans, to inhibit digestive enzymes involved in managing these conditions. We chemically characterized alginates confirmed their structural integrity using FTIR, NMR, TGA. The focus was on evaluating ability key relevant T2DM (α-amylase,...
Tumour metastasis remains the major cause of death in cancer patients and, to date, mechanism and signalling pathways governing this process are not completely understood. The TGF-β pathway is most commonly mutated cancer, however its role progression controversial as it can function both a promoter suppressor metastasis. Although previous studies have suggested for molecular chaperone Hsp90 regulating pathway, level at which occurs well consequences terms colon unknown. paired SW480 SW620...
The cancer stem cell (CSC) theory proposes that tumours arise from and are sustained by a subpopulation of cells with both properties. One the key hallmarks CSCs is ability to grow anchorage-independently under serum-free culture conditions resulting in formation tumourspheres. It has further been reported these resistant traditional chemotherapeutic agents.In this study, tumoursphere assay was validated MCF-7 used screen novel marine algal compounds for potential anti-cancer activity...
A tailored series of coumarin-based ferrocenyl 1,3-oxazine hybrid compounds was synthesized and investigated for potential antiparasitic activity, drawing inspiration from the established biological efficacy constituent chemical motifs. The structural identity confirmed by common spectroscopic techniques: NMR, HRMS IR. Biological evaluation studies reveal that exhibit higher in vitro potency against chemosensitive malarial strain (3D7 P. falciparum) over trypanosomiasis causal agent (T. b....
Human colorectal cancer (CRC) is a recurrent, deadly malignant tumour with high incidence. The incidence of CRC increasing alarm in highly developed countries, as well middle to low-income posing significant global health challenge. Therefore, novel management and prevention strategies are vital reducing the morbidity mortality CRC. Fucoidans from South African seaweeds were hot water extracted structurally characterised using FTIR, NMR TGA. fucoidans chemically analyse their composition. In...