A5085003029- Photodynamic Therapy Research Studies
- Porphyrin and Phthalocyanine Chemistry
- Nanoplatforms for cancer theranostics
- Heat shock proteins research
- Luminescence and Fluorescent Materials
- Cytokine Signaling Pathways and Interactions
- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Computational Drug Discovery Methods
- Monoclonal and Polyclonal Antibodies Research
- Adipokines, Inflammation, and Metabolic Diseases
- Transgenic Plants and Applications
- Synthesis and biological activity
- Glycosylation and Glycoproteins Research
- Bioactive Compounds and Antitumor Agents
- Protein purification and stability
- Advanced Nanomaterials in Catalysis
- Cancer, Hypoxia, and Metabolism
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nanocluster Synthesis and Applications
- Adipose Tissue and Metabolism
- Neuroscience and Neural Engineering
- Pluripotent Stem Cells Research
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Fluorescence Microscopy Techniques
Rhodes University
2015-2024
Bridge University
2022
University of Pretoria
2022
Business Innovation Centre
2018-2021
Biotechnology Innovation Organization
2015
In-Q-Tel
2012
Nelson Mandela University
2005-2009
Synthetic biology has grown exponentially in the last few years, with a variety of biological applications. One emerging applications synthetic is to exploit link between microorganisms, biologics, and human health. To this link, it critical select effective tools for use appropriate microorganisms that would address unmet needs health through development new game-changing by complementing existing technological capabilities. Lactic acid bacteria (LAB) are considered chassis organisms can be...
Heat shock protein 90 (Hsp90) has been identified in the extracellular space and shown to chaperone a finite number of proteins involved cell migration invasion. We used chemical cross-linking immunoprecipitation followed by tandem mass spectrometry (MS/MS) isolate complex containing Hsp90 matrix fibronectin (FN) from breast cancer cells. Further analysis showed direct binding FN using an vitro co-immunoprecipitation assay, solid phase assay surface plasmon resonance (SPR) spectroscopy....
Heat shock proteins (Hsps) play an important role in the development and pathogenicity of malaria parasites. One most prominent functions Hsps is to facilitate folding other proteins. are thought a crucial when parasites invade their host cells during subsequent hepatocytes red blood cells. It that maintain proteostasis under unfavourable conditions encounter environment. Although heat protein 70 (Hsp70) capable independent some proteins, its functional cooperation with 90 (Hsp90)...
The role of molecular chaperones, among them heat shock proteins (Hsps), in the development malaria parasites has been well documented. Hsp70s are chaperones that facilitate protein folding. Hsp70 composed an N-terminal nucleotide binding domain (NBD), which confers with ATPase activity and a C-terminal substrate (SBD). In ADP-bound state, possesses high affinity for releases folded when it is bound to ATP. two domains connected by conserved linker segment. Hsp110 possess extended lid...
The rising incidence of cancer is a major concern globally being leading cause early mortality in most countries. This burden projected to increase 28.4 million cases worldwide by 2040 and yet, may be worsened continuous exposure risk factors due sheer shift from consumption natural nutrients. To reverse this trend, it imperative source novel anticancer metabolites plants that could minimize carcinogenic effects suppressing the proliferation survival cells. study aimed at characterizing...
Heat shock protein 70 (Hsp70) is a molecular chaperone that plays an important role in cellular proteostasis. Hsp70s are also implicated the survival and pathogenicity of malaria parasites. The main agent malaria, Plasmodium falciparum, expresses six Hsp70s. Of these, two (PfHsp70-1 PfHsp70-z) localize to parasite cytosol. Previously conducted gene knockout studies suggested PfHsp70-z essential, it has been demonstrated small-molecule inhibitors targeting PfHsp70-1 cause death. For this...
Six Hsp70-like genes are represented on the genome of Plasmodium falciparum. Of these two occur in cytosol: P. falciparum Hsp70-z (PfHsp70-z) and PfHsp70-1. PfHsp70-1 is a well characterised canonical Hsp70 that facilitates protein quality control crucial for development malaria parasites. There very little known about PfHsp70-z. However, PfHsp70-z to be essential implicated suppressing aggregation asparagine-rich proteins In addition, its expression at clinical stage correlates with disease...
Novel chloroindium(III) complexes of tetra(4-methylthiophenyl)porphyrin (2a) and tetra-2-thienylporphyrin (2b) dyes have been synthesized characterized. The main goal the project was to identify fully symmetric porphyrin with Q-band regions that lie partially in therapeutic window are suitable for use photodynamic therapy (PDT). 2a 2b were found fluorescence quantum yield values [Formula: see text] 0.01 moderately high singlet oxygen yields (0.54−0.73) due heavy atom effects associated...
Abstract Heat shock protein 90 (Hsp90) functionally modulates signal transduction. The transducer and activator of transcription 3 (STAT3) mediates interleukin‐6 family cytokine signaling. Aberrant activation mutation STAT3 is associated with oncogenesis immune disorders, respectively. Hsp90 have previously been shown to colocalize coimmunoprecipitate in common complexes. Surface plasmon resonance spectroscopy revealed a direct, high affinity specific interaction between recombinant Hsp90β...
Plasmodium falciparum, the human pathogen responsible for most dangerous malaria infection, survives and develops in mature erythrocytes through export of proteins needed remodelling host cell. Molecular chaperones heat shock protein (Hsp) family are prominent members exportome, including a number Hsp40s Hsp70. PFA0660w, type II Hsp40, has been shown to be exported possibly form complex with PfHsp70-x infected erythrocyte cytosol. However, chaperone properties PFA0660w its interaction...
The photophysical properties and photodynamic therapy (PDT) activity of an axially ligated <italic>meso</italic>-thienyl Sn(<sc>iv</sc>) porphyrin are investigated.
A gold–chitosan composite with low symmetry zinc phthalocyanine showed no dark cytotoxicity activity and improved photodynamic therapy compared to that of the alone.
A tin(<sc>iv</sc>)corrole with <italic>meso</italic>-thien-2-yl rings has significantly red-shifted Q bands and is found to have favourable photodynamic therapy activity.