A5016011384- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Neuroinflammation and Neurodegeneration Mechanisms
- Stroke Rehabilitation and Recovery
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Sensorless Control of Electric Motors
- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- interferon and immune responses
- Neurogenesis and neuroplasticity mechanisms
- IL-33, ST2, and ILC Pathways
- Galectins and Cancer Biology
- Studies on Chitinases and Chitosanases
- Dermatology and Skin Diseases
- Fuzzy Logic and Control Systems
- Immune responses and vaccinations
- Cerebral Palsy and Movement Disorders
- Balance, Gait, and Falls Prevention
- Muscle activation and electromyography studies
- Enzyme Production and Characterization
- Lymphatic System and Diseases
- RNA regulation and disease
- Cervical Cancer and HPV Research
Brigham and Women's Hospital
2022-2025
Harvard University
2022-2025
Dongshin University
2013-2024
Hanyang University
2015-2023
Korean Society of Ginseng
2015
Chungbuk National University
2011-2013
Cell-cell interactions in the central nervous system play important roles neurologic diseases. However, little is known about specific molecular pathways involved, and methods for their systematic identification are limited. Here, we developed a forward genetic screening platform that combines CRISPR-Cas9 perturbations, cell coculture picoliter droplets, microfluidic-based fluorescence-activated droplet sorting to identify mechanisms of cell-cell communication. We used SPEAC-seq (systematic...
Abstract Central nervous system (CNS)-infiltrating effector T cells play critical roles in the development and progression of multiple sclerosis (MS). However, current drugs for MS are very limited due to difficulty delivering into CNS. Here we identify a cell-permeable peptide, dNP2, which efficiently delivers proteins mouse human cells, as well various tissues. Moreover, it enters brain tissue resident through blood vessels by penetrating tightly organized blood–brain barrier. The...
Abstract Chitinase-3-like-1 (Chi3l1) is known to play a significant role in the pathogenesis of Type 2 inflammation and cancer. However, function Chi3l1 T cell its clinical implications are largely unknown. Here we show that expression was increased activated cells, especially Th2 cells. In addition, Chi3l1-deficient cells hyper-responsive TcR stimulation prone differentiating into Th1 anti-tumor immunity genes decreased negative regulators. Deletion mice reduced melanoma lung metastasis...
Abstract T cells generate antigen-specific immune responses to their cognate antigen as a hallmark of adaptive immunity. Despite the importance cells, here we show that non-related, bystander memory-like CD4 + also significantly contribute autoimmune pathogenesis. Transcriptome analysis demonstrates interleukin (IL)-1β- and IL-23-prime express pathogenic Η 17 signature genes such RORγt, CCR6, granulocyte macrophage colony-stimulating factor (GM-CSF). Importantly, when co-transferred with...
Thymic epithelial cells (TECs) orchestrate T cell development by imposing positive and negative selection on thymocytes. Current studies TEC biology are hampered the absence of long-term ex vivo culture platforms, while driving self-renewal remain to be identified. Here, we generate (>2 years) expandable 3D organoids from adult mouse thymus. For further analysis, generated single double FoxN1-P2A-Clover, Aire-P2A-tdTomato, Cldn4-P2A-tdTomato reporter lines CRISPR knockin. Single-cell...
[Purpose] To determine the effect of dual-task training with cognitive tasks on and walking ability after stroke. [Subjects Methods] Twenty patients diagnosed stroke participated in this study. All participants were receiving a traditional rehabilitation program 5 days week. Dual-task single-task additionally performed for 4 weeks, 3 The Stroop test, Timed Up Go (TUG) 10-Meter Walk Test (10MWT), Figure-of-8 (F8WT) used to measure abilities evaluated times (before at 2-week follow-up)....
[Purpose] The purpose of this study was to examine the effects on stroke patients trunk stabilization exercise different support surfaces. [Subjects and Methods] Sixteen with onset six months earlier or longer were randomly equally assigned group I (exercise performed a stable surface) II an unstable surface). two groups conducted exercises respective surfaces, in addition existing rehabilitation five times per week for 12 weeks. Changes cross-sectional area (CSA) muscles examined using...
Regulatory T cells play a key role in immune tolerance to self-antigens, thereby preventing autoimmune diseases. However, no drugs targeting Treg have been approved for clinical trials yet. Here, chimeric peptide is generated by conjugation of the cytoplasmic domain CTLA-4 (ctCTLA-4) with dNP2 intracellular delivery, dNP2-ctCTLA-4, and evaluated Foxp3 expression during Th0, Th1, Treg, Th17 differentiation dependent on TGF-β. The lysine motif ctCTLA-4, not tyrosine motif, required induction...
[Purpose] Weakening of trunk muscles in stroke patients hinders functional ability, safety and balance. To confirm whether strengthening could facilitate rehabilitation patients, we investigated the effectiveness sling exercise therapy (SET) using closed kinetic chain exercises to activate improve balance patients. [Subjects Methods] Twenty with chronic hemiplegia were equally divided into 2 groups, a SET group control that performed regular on mat assistance table. Patients both groups...
Abstract Memory-phenotype (MP) CD4 + T cells are a substantial population of conventional that exist in steady-state mice, yet their immunological roles autoimmune disease remain unclear. In this work, we unveil unique phenotype MP determined by analyzing single-cell transcriptomic data and cell receptor (TCR) repertoires. We found the spleen were composed heterogeneous effector subpopulations existed regardless germ food antigen exposure. Distinct specifically activated IL-1 family...
Nuclear factor of activated T cells (NFATs) is an important transcription for cell activation and proliferation. Recent studies have highlighted the role NFATs in regulating differentiation effector CD4 helper (Th) subsets including Th1 Th17 cells. Because controlling function treatment autoimmune diseases, regulation NFAT functions would be strategy to control pathogenesis diseases. Here, we demonstrated that inhibitory peptide, VIVIT conjugated dNP2 (dNP2-VIVIT), a blood-brain...
본 연구는 PNF 결합패턴이 뇌졸중 환자의 마비측 하지 근 활성도와 보행 능력에 어떠한 영향을 미치는 지를 알아보고자 실시하였다. 환자 20명을 대상으로 하여 결합패턴(주 4회, 6주간)을 적용하는 실험군(n=10)과 일반 운동치료만을 대조군(n=10)으로 무작위 배정하였다. 활성도 측정은 활동전위의 실효치 값으로 분석하였고, 보행능력검사는 10MWT, DGI, F8WT를 이용하여 측정하였다. 운동 적용 방법에 따른 측정 시점에 대한 대조군과 실험군의 유의성 검정은 Two-way repeated measure ANOVA를 그 결과, 활성도를 알아보기 위해 측정한 RF(p<.05), VM, TA, LH, LG(p<.001) 근육 모두 각 시기에서 통계적으로 유의한 증가가 있었으며, LG(p<.001)에서 측정시간과 군 간의 상호작용이 나타났다. 보행능력을 실시한 F8WT 검사에서도 향상이 있었으며(p<.001), 10MWT(p<.05), DGI(p<.001),...
Curcumin is a natural product extracted from Curcuma longa. It has been reported as potent antioxidant and anti-inflammatory compound. Previous studies have demonstrated that curcumin suppresses pro-inflammatory cytokine production via inhibition of NF-κB in macrophages. However, its role adaptive immune cells such T cells, vivo, not clearly elucidated. Here, we examined the effects follicular helper (TFH) on Ab during NP-ovalbumin immunization mice. The results revealed administered daily...