A5009686073- Retinal Development and Disorders
- CRISPR and Genetic Engineering
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Glycosylation and Glycoproteins Research
- Retinal Diseases and Treatments
- Pluripotent Stem Cells Research
- RNA modifications and cancer
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- Ocular Infections and Treatments
- Hearing, Cochlea, Tinnitus, Genetics
- Neuroscience and Neural Engineering
- Advanced biosensing and bioanalysis techniques
- Ubiquitin and proteasome pathways
- Immune Response and Inflammation
- Glaucoma and retinal disorders
- RNA Research and Splicing
- Photoreceptor and optogenetics research
- Neuroinflammation and Neurodegeneration Mechanisms
- Vestibular and auditory disorders
Ministry of Education of the People's Republic of China
2024
Northwest University
2024
The Affiliated Yongchuan Hospital of Chongqing Medical University
2020-2021
Chongqing Medical University
2020-2021
Affiliated Eye Hospital of Wenzhou Medical College
2015-2018
Wenzhou Medical University
2015-2018
Retinitis pigmentosa (RP) is an irreversible, inherited retinopathy in which early-onset nyctalopia observed. Despite the genetic heterogeneity of RP, RPGR mutations are most common causes this disease. Here, we generated induced pluripotent stem cells (iPSCs) from three RP patients with different frameshift gene, were then differentiated into retinal pigment epithelium (RPE) and well-structured organoids possessing electrophysiological properties. We observed significant defects...
Abstract The pathophysiology of polycystic ovary syndrome (PCOS) is characterized by granulosa cell (GC) dysfunction. m 6 A modification affects GC function in patients with premature ovarian insufficiency (POI), but the role PCOS unknown. purpose prospective comparative study was to analyse profile luteinized GCs from normovulatory women and non‐obese following controlled hyperstimulation. RNA methylation levels were measured quantification assay controls patients. Then, profiles analysed...
Abstract The functions of macrophages are governed by distinct polarization phenotypes, which can be categorized as either anti-tumor/M1 type or pro-tumor/M2 type. Glycosylation is known to play a crucial role in various cellular processes, but its influence on macrophage not well-studied. In this study, we observed significant decrease bisecting GlcNAc during M0-M1 polarization, and impaired was found drive polarization. Using glycoproteomics strategy, identified Lgals3bp specific...
Objectives Infantile nystagmus (IN) is a genetically heterogeneous condition characterised by involuntary rhythmic oscillations of the eyes accompanied different degrees vision impairment. Two genes have been identified as mainly causing IN: FRMD7 and GPR143 . The aim our study was to identify genetic basis both sporadic IN X-linked IN. Design Prospective analysis. Patients Twenty Chinese patients, including 15 cases 5 from families, were recruited underwent molecular We first performed...
Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well-structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro. Moreover, mutation GTPase regulator (RPGR) gene was corrected by CRISPR-Cas9 editing, which rescued structure function of retinae. For complete details on use execution please refer to Deng et al. (2018).
Purpose: Accumulating evidence has demonstrated that excessive immunoreaction plays a prominent role in the pathogenesis of dry AMD. Toll-like receptor 3 (TLR3) can be activated by double-stranded (ds)RNA retinal pigment epithelia and trigger an innate immunity-mediated inflammatory response. However, its photoreceptor cells, effectors AMD geographic atrophy, remains unclear. Methods: The expression TLR3 was examined mouse retina murine cell line (661W). Retinal structure, function, death...