A5037721563- Pluripotent Stem Cells Research
- Tuberous Sclerosis Complex Research
- PI3K/AKT/mTOR signaling in cancer
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Mesenchymal stem cell research
- CRISPR and Genetic Engineering
- Microtubule and mitosis dynamics
- Tissue Engineering and Regenerative Medicine
- Ubiquitin and proteasome pathways
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Mast cells and histamine
- Polyamine Metabolism and Applications
- Vascular Tumors and Angiosarcomas
- Kruppel-like factors research
- Cancer-related Molecular Pathways
- Protein Kinase Regulation and GTPase Signaling
- Congenital Anomalies and Fetal Surgery
- Hippo pathway signaling and YAP/TAZ
- DNA Repair Mechanisms
- Biomedical Ethics and Regulation
- Congenital heart defects research
- RNA and protein synthesis mechanisms
- Histiocytic Disorders and Treatments
Medical University of Vienna
2013-2024
Medical Genetics Center
2012
Vienna General Hospital
2004-2005
University of Vienna
1973-2004
University of Michigan
1973
The mammalian target of rapamycin (mTOR) is part two distinct complexes, mTORC1, containing raptor and mLST8, mTORC2, rictor, mLST8 sin1. Although great endeavors have already been made to elucidate the function regulation mTOR, cytoplasmic nuclear distribution mTOR complexes unknown. Upon establishment proper experimental conditions, we found rictor sin1 be less abundant in nucleus than cytoplasm non-transformed, non-immortalized, diploid human primary fibroblasts. also high nucleus,...
Human amniotic fluid stem cells (hAFSCs) can be grown in large quantities, have a low risk for tumour development and harbour high differentiation potential. They are very promising new fetal cell type cell-based therapy approaches studying processes without raising the ethical concerns associated with embryonic cells. Recently, protocol studies on renal has been established which murine kidneys dissociated into single-cell suspension then reaggregated to form organotypic structures. Using...
In targeted therapy, patient tumors are analyzed for aberrant activations of core cancer pathways, monitored based on biomarker expression, to ensure efficient treatment. Thus, diagnosis and therapeutic decisions often the status biomarkers determined by immunohistochemistry in combination with other clinical parameters. Standard evaluation specimen is frequently impeded its dependence subjective interpretation, showing considerable intra- inter-observer variability. To make treatment more...
Recently, 2 branches of the wide area synthetic biology-in vitro gametogenesis and embryo development-have gained considerable attention. Rodent induced pluripotent stem cells derived via reprogramming somatic can in be differentiated into gametes to produce fertile offspring. And even embryos with organ progenitors were generated ex utero entirely from murine cells. The use these approaches basic research, which is rightfully accompanied by an ethical discussion, will allow hitherto...
Recently, amniotic fluid was suggested as a new source for stem-cell research and tissue engineering approaches. In order to enable isolation of stem cells establishment lines such with an undifferentiated phenotype we have introduced green fluorescent protein regulated by the promoters cell-specific genes, Oct-4 or Rex-1, into human cells. For introduction DNA cells, optimized specific transfection protocol. We found that contains cell populations which are able activate these promoters....
<i>TSC1</i> (tuberous sclerosis complex 1) encoding hamartin and <i>TSC2</i> tuberin are tumor suppressor genes responsible for the autosomal dominantly inherited disease tuberous sclerosis. These have been demonstrated to negatively regulate cell cycle progression, activity of cdk2, degradation cyclin-dependent kinase inhibitor p27. To date, underlying molecular mechanism remains elusive. Here, we show that binds Whereas also p27 in TSC1-negative cells, does not bind without tuberin....