A5039873197- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Genetics and Neurodevelopmental Disorders
- Neuroscience and Neural Engineering
- Cancer Mechanisms and Therapy
- NF-κB Signaling Pathways
- Neurogenesis and neuroplasticity mechanisms
- Phosphodiesterase function and regulation
- Ion Channels and Receptors
- Adipose Tissue and Metabolism
- Ion Transport and Channel Regulation
- Mitochondrial Function and Pathology
- Tryptophan and brain disorders
- Microtubule and mitosis dynamics
- RNA regulation and disease
- Signaling Pathways in Disease
- Memory and Neural Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Ubiquitin and proteasome pathways
- Adipokines, Inflammation, and Metabolic Diseases
- Cholinesterase and Neurodegenerative Diseases
- Mechanisms of cancer metastasis
- Barrier Structure and Function Studies
- Virus-based gene therapy research
- Peptidase Inhibition and Analysis
Korea Institute of Science and Technology
2018-2025
Korea Institute of Brain Science
2019-2025
Korean Association Of Science and Technology Studies
2019-2025
Korea Food Research Institute
2025
Korea University
2024
Korea Advanced Institute of Science and Technology
2024
Kyung Hee University
2017-2019
Two-pore domain K+ (K2P) channels have been shown to modulate neuronal excitability. The physiological role of TWIK-1, the first identified K2P channel, in cells is largely unknown, and we reported previously that TWIK-1 contributes intrinsic excitability dentate gyrus granule (DGGCs) mice. In present study, investigated coexpression TASK-3, another member, DGGCs. Immunohistochemical staining data showed TASK-3 proteins were highly localized proximal dendrites soma DGGCs, this localization...
Abstract K2P (two-pore domain potassium) channels, a diversified class of K + -selective ion have been found to affect wide range physiological processes in the body. Despite their established significance regulating proliferation and differentiation multiple cell types, channels’ specific role adipogenic (adipogenesis) remains poorly understood. In this study, we investigated engagement specifically KCNK2 (also known as TREK-1), adipogenesis using primary cultured adipocytes TREK-1 knockout...
Volume-regulated anion channels (VRACs) are involved in cellular functions such as regulation of cell volume, proliferation, migration, and death. Although leucine-rich repeat-containing 8A (LRRC8A) has been characterized a molecular component VRACs, here we show that Drosophila melanogaster tweety homologue 1 2 (TTYH1 TTYH2) critical for VRAC currents cancer cells. LRRC8A-independent were present the gastric line SNU-601, but almost completely absent its cisplatin-resistant derivative...
Calcium-activated chloride channels (CaCCs) mediate numerous physiological functions and are best known for the transport of electrolytes water in epithelia. In intestine, CaCC currents considered necessary secretion fluid to protect intestinal epithelium. Although genetic ablation ANO1/TMEM16A, a gene encoding CaCC, reduces carbachol-induced fluid, its mechanism action is still unknown. Here, we confirm that ANO1 essential fluid. Carbachol-induced transepithelial were reduced proximal colon...
TWIK-related potassium channel-1 (TREK-1) is broadly expressed in the brain and involved diverse diseases, such as seizures, ischemia, depression. However, cell type-specific roles of TREK-1 are largely unknown. Here, we generated a Cre-dependent knockdown (Cd-TREK-1 KD) transgenic mouse containing gene cassette for short hairpin ribonucleic acid to regulate expression. We confirmed by injecting adeno-associated virus (AAV) expressing Cre into hippocampus mice. To study role hippocampal...
TTYH2 is a calcium-activated, inwardly rectifying anion channel that has been shown to be related renal cancer and colon cancer. Based on the topological prediction, protein five transmembrane domains with extracellular N-terminus cytoplasmic C-terminus. In present study, we identified vesicle transport protein, β-COP, as novel specific binding partner of by yeast two-hybrid screening using human brain cDNA library C-terminal region (TTYH2-C) bait. Using in vitro vivo assays, confirmed...
Astrocytes, the most abundant cell type in brain, are non-excitable cells and play critical roles brain function. Mature astrocytes typically exhibit a linear current–voltage relationship termed passive conductance, which is believed to enable maintain potassium homeostasis brain. We previously demonstrated that TWIK-1/TREK-1 heterodimeric channels mainly contribute astrocytic conductance. However, molecular identity of conductance still controversial needs be elucidated. Here, we report...
TREK‐1 (TWIK‐related K+ channel), a member of the two‐pore domain (K2P) channel family, is highly expressed in astrocytes, where it plays key role glutamate release and passive conductance. In addition, induced to protect neurons under pathological conditions such as hypoxia. However, upstream regulation remains poorly understood. this study, we found that AEG‐1 (astrocyte elevated gene‐1) regulates expression astrocytic hypoxic conditions. Upregulation increased knockdown dramatically...
Abstract TWIK-1 is the first identified member of two-pore domain K+ (K2P) channel family, which plays an essential role in background conductance cells. However, physiological has remained largely unknown since phenotype previously reported exon 2-deleted Twik-1 knockout(KO) mice are not clear. Here, we demonstrate, through individual exon-specific short hairpin RNAs (shRNAs) against and CRISPR/Cas9 system to target each gene, that KO express unexpected functional channel. Furthermore,...
Abstract Mature hippocampal astrocytes exhibit a linear current-to-voltage (I-V) K + membrane conductance, which is called passive conductance. It estimated to enable keep potassium homeostasis in the brain. We previously reported that TWIK-1/TREK-1 heterodimeric channels are crucial for astrocytic However, regulatory mechanism of these by other binding proteins still remains elusive. Here, we identified Na+/H exchange regulator-1 (NHERF-1), protein highly expressed astrocytes, as candidate...
TWIK-1 is the first identified member of two-pore domain K+ (K2P) channel family, which plays an essential role in background conductance cells. Despite development exon 2-deleted Twik-1 knockout (KO) mice, physiological has remained largely unknown. Here, we observed that KO mice expressed internally deleted (TWIK-1 ΔEx2) protein, unexpectedly acts as a functional channel. A newly generated strain 1 was targeted using CRISPR/Cas9 technique provides strong evidence mediates currents are...
<title>Abstract</title> K2P (two-pore domain potassium) channels, a diversified class of K<sup>+</sup>-selective ion have been found to affect wide range physiological processes in the body. Despite their established significance regulating proliferation and differentiation multiple cell types, channels' specific role adipogenic (adipogenesis) remains poorly understood. In this study, we investigated engagement specifically KCNK2 (also known as TREK-1), adipogenesis using primary cultured...
ABSTRACT Mature hippocampal astrocytes exhibit a linear current‐to‐voltage (I‐V) K + membrane conductance called passive conductance. It is estimated to enable keep potassium homeostasis in the brain. We previously reported that TWIK‐1/TREK‐1 heterodimeric channels are crucial for astrocytic However, regulatory mechanism of these by other binding proteins remains elusive. Here, we identified Na+/H+ exchange regulator‐1 (NHERF‐1), protein highly expressed astrocytes, as novel interaction...
TWIK-1 belongs to the two-pore domain K+ (K2P) channel family, which plays an essential role in background conductance of cells. Despite development exon 2-deleted Twik-1 knockout (KO) mice, physiological has remained largely unknown. Here, we observed that KO mice expressed internally deleted (TWIK-1 ΔEx2) protein, unexpectedly acts as a functional channel. The nKO 1 was targeted using CRISPR-Cas9 technique provides strong evidence mediates currents are responsible for passive astrocytes....
AEG-1, also called MTDH, has oncogenic potential in numerous cancers and is considered a multifunctional modulator because of its involvement developmental processes inflammatory degenerative brain diseases. However, the role AEG-1 astrocytes remains unknown. This study aimed to investigate proteins directly regulated by analyzing their RNA expression patterns transfected with scramble shRNA shRNA. knockdown down-regulated TWIK-1 mRNA. Real-time quantitative PCR (qPCR) immunocytochemistry...