A5059987791- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Orthopaedic implants and arthroplasty
- Tendon Structure and Treatment
- Hematopoietic Stem Cell Transplantation
- Corneal surgery and disorders
- Corneal Surgery and Treatments
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- biodegradable polymer synthesis and properties
- Immunotherapy and Immune Responses
- Bone health and treatments
- Nerve Injury and Rehabilitation
- Muscle Physiology and Disorders
- Nerve injury and regeneration
- Metabolism and Genetic Disorders
- Microfluidic and Bio-sensing Technologies
- Facial Trauma and Fracture Management
- Nitric Oxide and Endothelin Effects
- Seaweed-derived Bioactive Compounds
- Tea Polyphenols and Effects
Inje University
2012-2021
The First Affiliated Hospital, Sun Yat-sen University
2021
Sun Yat-sen University
2021
Government of the Republic of Korea
2018
Beijing Forestry University
2017
This study aimed to develop an anti-inflammation system consisting of epigallo-catechin-3-gallate (EGCG) encapsulated in poly(lactide-co-glycolic acid) (PLGA) particles promote wound healing.Nano- and microscale PLGA were fabricated using a water/oil/water emulsion solvent evaporation method. The optimal particle size was determined based on drug delivery efficiency biocompatibility. loaded with EGCG. anti-inflammatory effects the evaluated vitro cell-based inflammation model.Nano- produced....
The roles of limbal epithelial stem cells (LESCs) are widely recognized, but for these to be utilized in basic research and potential clinical applications, researchers must able efficiently isolate them subsequently maintain their stemness vitro. We aimed develop a biomimetic environment LESCs involving from vivo niche the principle flow-induced shear stress, demonstrate this novel paradigm. LESCs, together with neighboring cells, were isolated minced tissues rabbits. At days 8 9 culture,...
Enhanced biocompatibility and osteogenic potential of mesoporous magnesium silicate/polycaprolactone/wheat protein composite scaffolds Yun Gyeong Kang,1 Jie Wei,2 Ji Won Shin,1 Yan Ru Wu,3 Jiacan Su,4 Young Shik Park,5 Jung-Woog Shin1,3,6 1School Biomedical Engineering, Inje University, Gimhae, Republic Korea; 2Key Laboratory for Ultrafine Materials Ministry Education, East China University Science Technology, Shanghai, China; 3Department Health 4Department Orthopaedics, Changhai Hospital,...
Mechanical stimuli play important roles in the proliferation and differentiation of adult stem cells. However, few studies on their effects induced pluripotent cells (iPSCs) have been published. Human dermal fibroblasts were seeded onto flexible membrane-bottom plates, infected with retrovirus expressing four reprogramming factors OCT4, SOX2, KLF, c-MYC (OSKM). The subjected to equiaxial stretching (3% or 8% for 2, 4, 7 days) feeder (STO). into iPSCs was evaluated by expression markers,...
We describe the ex vivo expansion of haematopoietic stem/progenitor cells (HSPCs) with consideration their eventual in-vivo niche. firstly fabricated hierarchically structured scaffolds (lattices derived via three-dimensional plotting combined electrospun submicron fibers coated vitronectin to increase cell affinity). also applied intermittent hydrostatic pressure (IHP) mimic physical environment in In absence mechanical stimuli, phenotype (CD34+, CD34+CD38-) remained excellent...
A new composite scaffold consisting of mesoporous magnesium–calcium silicate (m_MCS), polycaprolactone (PCL), and polybutylene succinate (PBSu) was manufactured by a rapid prototyping technique, for stem cell-based bone tissue engineering.
The roles of mitochondria in various physiological functions vascular endothelial cells have been investigated extensively. Morphological studies relation to performed. However, there few reports morphological investigations related stem cell differentiation. This was the first study differentiation and focused on quantitative analysis changes mitochondrial morphology, number, area, length during human mesenchymal (hMSCs) into endothelial-like cells. To induce differentiation, we engaged...
The tissue-engineered oesophagus serves as an alternative and promising therapeutic approach for long-gap oesophageal replacement. This study proposes advanced in vitro culture platform focused on construction of the by combining electrospun double-layered tubular scaffold, stem cells, biochemical reagents, biomechanical factors. Human mesenchymal cells were seeded onto inner outer surfaces scaffold. Mechanical stimuli applied with a hollow organ bioreactor along different reagents inside...
Abstract We investigated the structural complexity and texture of cytoskeleton nucleus in human mesenchymal stem cells during early phase differentiation into osteoblasts according to differentiation–induction method: mechanical and/or chemical stimuli. For this, fractal dimension a number parameters utilizing gray-level co-occurrence matrix (GLCM) were calculated based on single-cell images after confirmation by immunofluorescence staining. The F-actin nuclear dimensions greater both...
Ex vivo expansion of hematopoietic stem/progenitor cell (HSPC) has been investigated to improve the clinical outcome HSPC transplantation. However, ex HSPCs still faces a major obstacle in that HPSCs tend differentiate when proliferating. Here, we cocultured with mesenchymal stem cells (MSCs) and divided into two fractions according whether they came adherent MSCs or not. Additionally, used hydrostatic pressure (HP) mimic physical conditions . Even nonadherent expanded yield significantly...
Abstract Even the efficacy of substrate and mechanical stimuli in addition to biochemical cues have been recognized many studies stem cell differentiation, few reported on differentiation into esophageal epithelial cells. Therefore, aim this study was set propose a method differentiating cells according reagent concentration, properties, forces. After concentration all‐trans retinoic acid determined as 5 μ M by baseline experiment, degree compared three different kinds substrates: cover...