A5083412701- Chronic Lymphocytic Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Lymphoma Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- CAR-T cell therapy research
- Hematopoietic Stem Cell Transplantation
- Complement system in diseases
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- Hemoglobinopathies and Related Disorders
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Blood disorders and treatments
- Acute Lymphoblastic Leukemia research
- Viral-associated cancers and disorders
- Chronic Myeloid Leukemia Treatments
- GDF15 and Related Biomarkers
- Mesenchymal stem cell research
- Neurological Complications and Syndromes
- Glycosylation and Glycoproteins Research
- Renal Diseases and Glomerulopathies
- CNS Lymphoma Diagnosis and Treatment
- Galectins and Cancer Biology
- Corneal Surgery and Treatments
- T-cell and B-cell Immunology
G. Papanikolaou General Hospital
2016-2025
Centre for Research and Technology Hellas
2013-2022
Karolinska Institutet
2018-2020
Alexion Pharma (Switzerland)
2020
European Hematology Association
2020
Uppsala University
2013-2017
Hellenic Oncology Research Group
2016
Central European Institute of Technology
2013
San Raffaele University of Rome
2013
General Hospital of Nikea
2013
Recent transcriptomic-based analysis of diffuse large B cell lymphoma (DLBCL) has highlighted the clinical relevance lymph node (LN) fibroblast and tumor-infiltrating lymphocyte (TIL) signatures within tumor microenvironment (TME). However, immunomodulatory role fibroblasts in remains unclear. Here, by studying human mouse DLBCL-LNs, we identify presence an aberrantly remodeled fibroblastic reticular (FRC) network, expressing elevated activated protein (FAP). RNA-sequencing analyses reveal...
Subgroups of patients with chronic lymphocytic leukemia (CLL) have distinct expression profiles Toll-like receptor (TLR) pathway-associated genes. To test the hypothesis that signaling through innate immunity receptors may influence behavior malignant clone, we investigated functional response triggered by stimulation TLRs and NOD2 in 67 CLL cases assigned to different subgroups on basis immunoglobulin heavy variable (IGHV) gene usage, IGHV mutational status or B-cell (BcR) stereotypy....
Abstract Renewed interest has emerged in transplant‐associated thrombotic microangiopathy ( TA ‐ TMA ) with novel prognostic, diagnostic, and treatment algorithms. We aimed to investigate the incidence, prognostic factors, morbidity, mortality of allogeneic hematopoietic cell transplantation HCT recipients. enrolled consecutive recipients (1990‐2017). Among 758 patients, 116 (15.5%) were diagnosed . In multivariate analysis, TBI ‐based conditioning, viral infections, acute chronic GVHD...
Loss of microbiota diversity has been clearly associated with poor outcomes in the allogeneic hematopoietic stem cell transplantation setting. However, choice optimal antibiotic prophylaxis during pre-engraftment phase remains unclear. We designed a prospective randomized study to compare our standard-of-care neutropenia (ciprofloxacin) rifaximin. enrolled 38 consecutive adult patients who underwent setting and were randomly assigned receive ciprofloxacin (20 patients) or rifaximin (18 at...
Abstract Purpose: The role of antigen(s) in shaping the T-cell repertoire chronic lymphocytic leukemia, although relevant for understanding malignant cell interactions with cognate T cells, is largely unexplored. Experimental Design: Here we profiled receptor β chain gene 58 leukemia patients, focusing on cases assigned to well-characterized subsets stereotyped clonotypic B-cell immunoglobulins, therefore those most evidently selected by antigen (subsets #1, #2, and #4). Results: Remarkable...
Abstract Background Graft vs host disease (GVHD) is the most severe complication of allogeneic hematopoietic cell transplantation. Conventional immunosuppressive therapy increases morbidity and mortality without improving survival. Extracorporeal photopheresis (ECP) has been introduced as an alternative treatment in steroid‐dependent steroid‐refractory disease. Study design methods We studied safety efficacy ECP a second‐ or third‐line GVHD. Results was administered 21 patients with grade...
Transplant-associated thrombotic microangiopathy (TA-TMA) and graft-versus-host disease (GVHD) are complications of allogeneic hematopoietic cell transplantation (HCT) that share several common characteristics.1 Both affect multiple organs lead to significant morbidity mortality despite optimal management. Despite a distinct clinical phenotype, both manifest in the early posttransplant period have denominator: endothelial damage.2 Endothelial injury has been long recognized as contributor...
(1) Background: Autologous, allogeneic hematopoietic cell transplantation (HCT) and other cellular therapies, including CAR T gene therapy, constitute a cornerstone in the management of various benign malignant hematological disorders. Invasive fungal infections (IFD) remain significant cause morbidity mortality HCT recipients. Therefore, we investigated prevalence risk factors IFD following therapies an era novel antifungal prophylaxis. (2) Methods: In this study, retrospectively enrolled...
Immunoglobulin G-switched chronic lymphocytic leukemia (G-CLL) is a rare variant of CLL, whose origin and ontogenetic relationship to the common IgM/IgD (MD-CLL) remains undefined. Here, we sought for clues about ontogeny G-CLL versus MD-CLL by profiling relevant IG gene repertoires.Using purpose-built bioinformatics methods, performed detailed immunogenetic multinational CLL cohort comprising 1,256 cases, which 1,087 169 expressed mu/delta gamma heavy chains, respectively.G-CLL has highly...
Analysis of the T cell receptor (TR) repertoire chronic lymphocytic leukemia-like monoclonal B lymphocytosis (CLL-like MBL) and early stage CLL is relevant for understanding dynamic interaction expanded clones with bystander cells. Here we profiled β chain (TRB) CD4+ CD8+ fractions from 16 CLL-like MBL 13 untreated, Binet A/Rai 0 patients using subcloning analysis followed by Sanger sequencing. The subpopulations both harbored restricted TRB gene repertoire, clonal expansions whose frequency...
Abstract Purpose: Using next-generation sequencing (NGS), we recently documented T-cell oligoclonality in treatment-naïve chronic lymphocytic leukemia (CLL), with evidence indicating selection by restricted antigens. Experimental Design: Here, sought to comprehensively assess repertoire changes during treatment relation (i) type [fludarabine-cyclophosphamide-rituximab (FCR) versus ibrutinib (IB) rituximab-idelalisib (R-ID)], and (ii) clinical response, combining NGS immunoprofiling, flow...
Endothelial injury indices, such as Activation and Stress Index (EASIX), modified EASIX (m-EASIX), simplified (s-EASIX) scores, have been previously associated with chimeric antigen receptor-T (CAR-T) cell immunotherapy complications. Soluble urokinase-type plasminogen activator receptor (suPAR), growth differentiation factor-15 (GDF-15), soluble C5b-9 (sC5b-9) described markers of endothelial post-hematopoietic stem transplantation. In the current study, we examined whether suPAR, GDF-15,...
(1) Background: survivors of allogeneic hematopoietic cell transplantation (alloHCT) suffer from morbidity and mortality due to cardiovascular events. We hypothesized that vascular injury pro-coagulant activity are evident in alloHCT without existing complications or relapse. (2) Methods: we enrolled consecutive adult established disease control individuals matched for traditional risk factors (January–December 2019). Circulating microvesicles (MVs) different cellular origins (platelet,...