A5106856986- Pluripotent Stem Cells Research
- Nuclear Receptors and Signaling
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Animal Genetics and Reproduction
- Neuroinflammation and Neurodegeneration Mechanisms
- Tissue Engineering and Regenerative Medicine
- Parkinson's Disease Mechanisms and Treatments
- Genetics and Neurodevelopmental Disorders
- Virus-based gene therapy research
- Metabolism and Genetic Disorders
- Neurological Disease Mechanisms and Treatments
- Neurological disorders and treatments
- Mesenchymal stem cell research
- Melanoma and MAPK Pathways
- RNA Interference and Gene Delivery
- Muscle Physiology and Disorders
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Amino Acid Enzymes and Metabolism
- Ubiquitin and proteasome pathways
- 14-3-3 protein interactions
- Cell death mechanisms and regulation
- Nuclear Structure and Function
Chinese Institute for Brain Research
2024
Capital Medical University
2006-2018
Capital University
1997-2015
Hypoxic preconditioning (HPC) initiates intracellular signaling pathway to provide protection against subsequent cerebral ischemic injuries, and its mechanism may molecular targets for therapy in stroke. According our study of conventional protein kinase C βII (cPKCβII) activation HPC, the role cPKCβII HPC-induced neuroprotection interacting proteins were determined this study. The autohypoxia-induced HPC middle artery occlusion (MCAO)-induced ischemia mouse models prepared as reported. We...
Parkinson's disease (PD) is characterized by selective and progressive degeneration of dopaminergic neurons in the substantia nigra (SN). Lipopolysaccharide (LPS) can induce chronic inflammation has been widely used to study pathogenesis PD. In this study, a single intracerebroventricular injection LPS was neurotoxic effects on Sprague-Dawley rats. The long-term were evaluated at different time points. Microglia activated hippocampus striatum 4 weeks, SN 24 weeks. Astrocytes nigrostriatal...
Melanoma is the main cause of death associated with skin cancer. Surgical resection and adjuvant therapy are currently effective treatments, but recurrence rate very high. The understanding microRNA (miR) dynamics after surgical melanoma essential to accurately explain changes in melanoma. In this study, we hypothesized that microRNA-23a (miR-23a) affects cell proliferation, migration, invasion a mechanism related SDCBP MAPK/ERK signaling pathway. To validate this, performed series...
Polo-like kinase 1 (Plk1) is pivotal for proper mitotic progression, its targeting activity regulated by precise subcellular positioning and phosphorylation. Here we assessed the protein expression, localization possible functions of phosphorylated Plk1 (pPlk1Ser137 pPlk1Thr210) in mouse oocytes during meiotic division. Western blot analysis revealed a peptide pPlk1Ser137 with high stable expression from germinal vesicle (GV) until metaphase II (MII), while pPlk1Thr210 was detected as one...
Background: Nurr1, a member of the nuclear receptor superfamily transcription factors, is highly expressed in midbrain dopaminergic (DA) neurons. Ret tyrosine kinase (RTK) and critical signal transducing subunit receptors for glial cell line-derived neurotrophic factor (GDNF). Both Nurr1 play important roles development DA Purpose: To investigate possible correlation between on inducing expression hydroxylase (TH) neural precursor cells. Methods: Neural precursors isolated from rat embryonic...
Nerve regeneration and circuit reconstruction remain a challenge following spinal cord injury (SCI). Corticospinal pyramidal neurons possess strong axon projection ability. In this study, human induced pluripotent stem cells (iPSCs) were differentiated into neuronal precursors (PNPs) by addition of small molecule dorsomorphin the culture. iPSC-derived PNPs transplanted acutely rat contusion SCI model on same day injury. Following engraftment, rats showed significantly improved motor...
Objective To detect the expression and function of enzyme genes involved in biosynthetic pathway for dopamine vitro assess their effect rat model Parkinson's disease. Methods Cos7 cells were transfected with separate adeno-associated virus (AAV) expressing tyrosine hydroxylase (TH) gene, aromatic L-amino acid decarboxylase (AADC) gene GTP cyclohydrolase I (GCH-I) gene. The three detected by methods immunohistochemistry, situ hybridization high performance liquid chromatograph electrochemical...
Neural progenitor cells from human brain are crucial for successful development of approaches to cell therapy neurodegenerative diseases including Alzheimer s disease. The objectives this study investigate whether immortalized neural (hSN12W-TERT cells) can be induced differentiate toward a neuronal phenotype by all-trans retinoic acid (RA), and there is any functional link between p27Kip1 function RA in the control growth arrest differentiation hSN12W-TERT cells. Human derived striatums...