A5016595587- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Research on Leishmaniasis Studies
- Helminth infection and control
- Immune Cell Function and Interaction
- Trace Elements in Health
- Parasitic Infections and Diagnostics
- Phytochemical compounds biological activities
- Reproductive System and Pregnancy
- Insect Pest Control Strategies
- Immune Response and Inflammation
- African history and culture studies
- Autophagy in Disease and Therapy
- Genetics, Aging, and Longevity in Model Organisms
- Tuberculosis Research and Epidemiology
- Liver Disease and Transplantation
- Autoimmune and Inflammatory Disorders Research
- Vitamin D Research Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Coccidia and coccidiosis research
- Neurobiology and Insect Physiology Research
- Computational Drug Discovery Methods
- Malaria Research and Control
- Insect and Pesticide Research
- Synthesis and bioactivity of alkaloids
Justus-Liebig-Universität Gießen
2017-2025
Universitätsklinikum Erlangen
2011-2018
Leiden University Medical Center
2014-2017
Loyola University Medical Center
2015
Friedrich-Alexander-Universität Erlangen-Nürnberg
2007-2013
Institute of Medical Microbiology and Hygiene
2009
German Cancer Research Center
2009
Heidelberg University
2009
Ludwig-Maximilians-Universität München
2009
Mater Research
2009
Chronic low-grade inflammation associated with obesity contributes to insulin resistance and type 2 diabetes. Helminth parasites are the strongest natural inducers of immune responses, short-lived infection rodent nematodes was reported improve glucose tolerance in obese mice. Here, we investigated effects chronic (12 weeks) Schistosoma mansoni, a helminth that infects millions humans worldwide, on whole-body metabolic homeostasis white adipose tissue (WAT) cell composition high-fat...
Infection with the helminth Schistosoma (S.) mansoni drives development of interleukin (IL)-10-producing regulatory B (Breg) cells in mice and man, which have capacity to reduce experimental allergic airway inflammation are thus high therapeutic interest. However, both involved antigen cellular mechanisms that drive Breg cell remain be elucidated. Therefore, we investigated whether S. soluble egg antigens (SEA) directly interact enhance their potential, or act indirectly on via SEA-modulated...
We report the in vitro screening of bis-3-chloropiperidine derivatives against free-living Caenorhabditis elegans and parasitic Schistosoma mansoni . 56% compounds active C. were also S.
ABSTRACT Natural killer (NK) cells are important components of a protective immune response against intracellular pathogens such as Leishmania parasites, which reside within myeloid cells. Previous in vivo studies murine cutaneous or visceral leishmaniasis showed that NK activated by conventional dendritic Toll-like receptor 9-, interleukin-12 (IL-12)-, and IL-18-dependent manner during the early phase infection help to restrict tissue parasite burden unknown mechanisms. Here, we tested...
Schistosomiasis is an infectious disease caused by blood flukes of the genus Schistosoma and affects approximately 200 million people worldwide. Since Praziquantel (PZQ) only drug for schistosomiasis, alternatives are needed. By a biochemical approach, we identified tegumentally expressed aldehyde dehydrogenase (ALDH) S. mansoni, SmALDH_312. Molecular analyses adult parasites showed Smaldh_312 transcripts in both genders different tissues. Physiological cell-biological experiments exhibited...
Activation of NK cells is a hallmark infections with intracellular pathogens. We previously showed that the protozoan parasite Leishmania infantum triggered rapid NK-cell response in mice required TLR9-positive myeloid DC and IL-12, but no IFN-alpha/beta. Here, we investigated whether IL-15 or IL-18 mediate activity IL-12 function as independent activators cells. In contrast to earlier studies described crucial for priming TLR ligands, expression IFN-gamma, FasL, perforin granzyme B by L....
Natural products have moved into the spotlight as possible sources for new drugs in treatment of helminth infections including schistosomiasis. Surprisingly, insect-derived compounds largely been neglected so far search novel anthelminthics, despite generally recognized high potential insect biotechnology drug discovery. This motivated us to assess antischistosomal capacity harmonine, an antimicrobial alkaloid from harlequin ladybird Harmonia axyridis that raised interest recent years. We...
Abstract Schistosoma mansoni is a parasitic flatworm causing schistosomiasis, an infectious disease affecting several hundred million people worldwide. Schistosomes live dioeciously, and upon pairing with the male, female starts massive egg production, which causes pathology. Praziquantel (PZQ) only drug used, but it has inherent risk of resistance development. Therefore, alternatives are needed. In context repurposing, cancer imatinib was tested, showing high efficacy against S. in vitro....
Abstract In parasites such as Schistosoma mansoni , gene knockdown by RNA interference (RNAi) has become an indispensable tool for functional characterization. To distinguish target-specific RNAi effects versus off-target effects, controls are essential. date, however, there is still no general agreement about suitable controls, which limits the comparability between studies. address this point, we investigated three selected dsRNAs their suitability in experiments with adult S. vitro. Two...
Knowledge on the cell types and cell-specific gene expression of multicellular pathogens facilitates drug discovery allows gaining a deeper understanding pathogen biology. By utilizing single-cell RNA sequencing (scRNA-seq), we analyzed 19,581 cells globally prevalent parasitic flatworm, liver fluke Fasciola hepatica, which causes neglected tropical disease zoonosis known as fascioliasis. We identified 15 distinct clusters, including reproductive tract gastrodermis, report identification...
The spatial organization of gene expression dictates tissue functions in multicellular parasites. Here, we present the transcriptome a parasitic flatworm, common liver fluke Fasciola hepatica. We identify profiles and marker genes for eight distinct tissues validate latter by situ hybridization. To demonstrate power our atlas, focus on with substantial medical importance, including vaccine candidates (Ly6 proteins) drug resistance (glutathione S-transferases, ABC transporters). Several these...
Schistosomiasis is a neglected tropical disease caused by blood flukes of the genus Schistosoma. In silico screenings compounds for identification novel anti-parasitic drug candidates have received considerable attention in recent years, including screening natural compounds. For first time, we investigated molecules from insects, rather source discovery, an approach to find antischistosomal Based on Dictionary Natural Products (DNP), created library 1327 insect suitable molecular docking. A...
Abstract Inhibition of eukaryotic initiation factor 4A has been proposed as a strategy to fight pathogens. Rocaglates exhibit the highest specificities among eIF4A inhibitors, but their anti-pathogenic potential not comprehensively assessed across eukaryotes. In silico analysis substitution patterns six eIF4A1 aa residues critical rocaglate binding, uncovered 35 variants. Molecular docking eIF4A:RNA:rocaglate complexes, and in vitro thermal shift assays with select recombinantly expressed...
SUMMARY Cercariae of Diplostomum spathaceum penetrate the skin fish, and then migrate along blood vessels tissues towards head eye-lens. We studied their orientation behaviour in tail fins guppies chemical concentration gradients within agar-filled choice chambers. In fins, they entered veins orientated cranially, independent flow living cells. chambers, were attracted by a small molecular fraction fish serum, D-glucose (at 1, 10, 1000 μ m ), D-mannose, D-maltotriose Cl-ions, whereas...
Leishmaniasis is caused by protozoa of the genus Leishmania and transmitted sand flies from mammalian reservoirs to humans. In recent years, a northward spread L. infantum highly endemic Mediterranean countries into previously non-endemic Central European areas has been suspected based on presumed sporadic cases autochthonous leishmaniasis. Here, we investigated whether are prevalent in Bavaria Southern Germany, federal state which have reported. Considering present future climatic...
In humans and murine models of malaria, intradermal immunization (ID-I) with genetically attenuated sporozoites that arrest in liver induces lower protective immunity than intravenous (IV-I). It is unclear whether this difference caused by fewer migrating into the or suboptimal hepatic injection site-dependent immune responses. We therefore developed a Plasmodium yoelii immunization/boost/challenge model to examine parasite loads as well lymph node responses protected unprotected ID-I IV-I...