A5100409191- CRISPR and Genetic Engineering
- Alzheimer's disease research and treatments
- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Real-time simulation and control systems
- Neuroscience and Neuropharmacology Research
- Electric and Hybrid Vehicle Technologies
- Plant and animal studies
- Hydraulic and Pneumatic Systems
- Pain Mechanisms and Treatments
- Mesenchymal stem cell research
- Cytomegalovirus and herpesvirus research
- Neuropeptides and Animal Physiology
- Plant and Fungal Species Descriptions
- Cholinesterase and Neurodegenerative Diseases
- Evolution and Genetic Dynamics
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Safety and Risk Management
- Underground infrastructure and sustainability
- Transgenic Plants and Applications
- Circular RNAs in diseases
- Nutrition and Health in Aging
Xuzhou Medical College
2024
Massachusetts Institute of Technology
2024
Shaoxing People's Hospital
2024
University of California, Berkeley
2023
Innovative Genomics Institute
2023
Army Medical University
2022
Shanghai East Hospital
2020
California Academy of Sciences
2018-2019
University of California, San Francisco
2009-2018
San Francisco State University
2018
Apolipoprotein E4 (apoE4) is the major genetic risk factor for Alzheimer's disease. However, underlying mechanisms are unclear. We found that female apoE4 knock-in (KI) mice had an age-dependent decrease in hilar GABAergic interneurons correlated with extent of learning and memory deficits, as determined Morris water maze, aged mice. Treating apoE4-KI daily peritoneal injections GABA A receptor potentiator pentobarbital at 20 mg/kg 4 weeks rescued deficits. In neurotoxic fragment transgenic...
CRISPR-Cas adaptive immune systems capture DNA fragments from invading mobile genetic elements and integrate them into the host genome to provide a template for RNA-guided immunity1. CRISPR maintain integrity avoid autoimmunity by distinguishing between self non-self, process which CRISPR/Cas1-Cas2 integrase is necessary but not sufficient2-5. In some microorganisms, Cas4 endonuclease assists adaptation6,7, many lack Cas48. Here we show here that an elegant alternative pathway in type I-E...
Protein synthesis and secretion are essential to cellular life. Although secretory activities may vary in different cell types, what determines the maximum capacity is inherently difficult study. Increasing protein until reaching limit of one strategy address this key issue. Under highly optimized growth conditions, recombinant CHO cells engineered produce a model human IgG clone started housing rod-shaped crystals endoplasmic reticulum (ER) lumen. The intra-ER crystal was accompanied by...
A new enhancer of zeste homolog 2 (EZH2) inhibitor series comprising a substituted phenyl ring joined to dimethylpyridone moiety via an amide linkage has been designed. preferential torsion that improved the binding properties compounds was identified for this computational analysis. Cyclization linker resulted in six-membered lactam analogue, compound 18. This transformation significantly ligand efficiency/potency cyclized relative its acyclic analogue. Additional optimization...
Changes in mitochondrial morphology and function play an important role secondary damage after acute spinal cord injury. We recorded the time representation of rats with Results showed that mitochondria had irregular shape, increased size. Mitochondrial cristae were disordered membrane rupture was visible at 2-24 hours Fusion protein mitofusin 1 expression gradually increased, peaked 8 injury, then decreased to its lowest level 24 hours. Expression dynamin-related 1, amitochondrial fission...
Alpha-7 nicotinic acetylcholine receptor (α7-nAChR) agonists can prevent glutamate-induced excitotoxicity in cultured retinal ganglion cells (RGCs). However, the neuroprotective effects and mechanism of action PNU-282987, an α7-nAChR agonist, a chronic vivo rat glaucoma model are poorly understood. We found that elevated intraocular pressure (IOP) downregulated expression. Electroretinography revealed amplitude photopic negative response (PhNR) decreased parallel with loss RGCs caused by...
Abstract Uncontrolled or chronic inflammation contributes to the pathogenesis of many acute/chronic diseases, such as acute organ injury, COVID‐19, and atherosclerosis. Intrinsically bioactive materials are promising for regulating response magnitude duration inflammation, but their translation remains challenging. Herein, engineering a series inflammation‐resolving by rationally integrating different functional modules into hydrolyzable scaffold is reported. The obtained can assemble potent...
In the era of combination therapy, there has been limited research on body composition. Specific composition, such as sarcopenia, possesses potential to serve a predictive biomarker for toxic effects and clinical response in patients with urothelial carcinoma (UC) undergoing tislelizumab combined gemcitabine cisplatin (T + GC).
Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with pathogenesis of Alzheimer's disease (AD). Here, we describe protocol for efficient knockout APOE human induced pluripotent stem cells (iPSCs) using CRISPR-Cas9 system. We obtain homozygous (APOE-/-) iPSCs further validate deficiency apoE iPSC-derived cerebral organoids. APOE-/- organoids can serve as useful tool to study functions apoE-related pathogenic mechanisms AD. For complete details on use...
Abstract CRISPR-Cas adaptive immune systems capture DNA fragments from invading mobile genetic elements and integrate them into the host genome to provide a template for RNA-guided immunity. CRISPR maintain integrity avoid autoimmunity by distinguishing between self non-self, process which CRISPR-Cas1:Cas2 integrase is necessary but not sufficient. In some microbes, Cas4 endonuclease assists adaptation, many lack Cas4. We show here that an elegant alternative pathway employs internal...
Drivability and fuel economy are continuously emphasized during the development of automobiles, shift quality is most important part drivability. To improve quality, integrated powertrain control strategy for gear shifting presented in this paper. First all, transmission based on turbine speed trajectory proposed. Furthermore, an output torque observer determined to implement coordinated engine control, then presented. Finally, studied by experiments. The experimental results show that...
Several taxonomic treatments of Styrax (Styracaceae) exist in regional floras Asia, but the Asian species genus have not been comprehensively revised since 1907. A treatment taxa S. series Cyrta with imbricate floral aestivation was accomplished 2003. To complete revision Cyrta, we conducted a valvate corolla lobes. Our comprises 11 combined distribution from eastern India through southern China and Malesia, Melanesia, Micronesia, although group is absent Philippines. We resurrected...
Geographic range extensions are documented for the Asian species Styrax japonicus and S. odoratissimus of series Cyrta. Implications extension in taxonomic distinctness phylogenetic relationships formosanus discussed.
Objective To investigate the effect of bilateral cervical vagotomy on microglial activation in spinal cord a rat model persistent postoperative pain evoked by skin/muscle incision and retraction (SMIR). Methods Thirty six male Sprague-Dawley rats were randomly divided into three groups (n= 12 each group): group sham operation, SMIR, SMIR+ (SV). The SMIR was established according to method described Flatter. Pain behavior assessed paw mechanical withdrawal threshold (MWT) von Frey...
To investigate whether the presence of multiple renal arteries in remnant kidney has implications for lower function or increased incidence hypertension.We reviewed intraoperative and follow-up data 101 live donors who underwent nephrectomies at our institution. Sixty-nine (68.3%) had single artery (Group A), while 32 (31.7%) B). We compared demographic between two groups. The each group were divided into three subgroups based on length period (12-24 months, 24-48 months ≥48 months)....