A5004320588- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Respiratory viral infections research
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Viral gastroenteritis research and epidemiology
- Malaria Research and Control
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- Virology and Viral Diseases
- vaccines and immunoinformatics approaches
Wonkwang University
2015-2024
Field diagnostic tools for avian influenza (AI) are indispensable the prevention and controlled management of highly pathogenic AI-related diseases.More accurate, faster networked on-site monitoring is demanded to detect such AI viruses with high sensitivity as well maintain up-to-date information about their geographical transmission.In this work, we assessed clinical field-level performance a smartphone-based fluorescent device an efficient reflective light collection module using...
The development of a sensitive and rapid diagnostic test is needed for early detection avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against A H7N9 recombinant hemagglutinin (rHA)1 were developed applied to Europium nanoparticle-based fluorescent immunochromatographic strip (FICT) improve the sensitivity system. Two (2F4 6D7) exhibited subtype specificity in dot-FICT assay by optimization conjugate pH lysis buffer. was confirmed an immunofluorescence...
Zika virus (ZIKV) has been identified as a cause of adverse outcomes pregnancy, including microcephaly and other congenital diseases.Most people infected with ZIKV do not show any symptoms.Development method to discriminate dengue (DENV) infections challenging, efficient assays for patient management are limited, attributable high levels cross-reactivity among co-circulating Flaviviruses.Thus, there is an urgent need specific high-throughput diagnostic assay from Flavivirus...
Dengue, one of the most prevalent illnesses caused by dengue viruses that are members genus Flavivirus, is a significant global health problem. However, similar clinical symptoms and high antigenic homologies with other Flaviviruses in endemic area pose difficulties for differential diagnosis from arbovirus infections. Here, we investigated four types recombinant envelope protein domain III (DV-rED III) derived virus (DENV) serotypes diagnostic potential detecting IgM acute phase (mainly 2-3...
Alternative antibody (aptamer)-based biosensors are attracting increasing attention owing to advantages such as simplicity and low cost, which beneficial for point-of-care diagnosis, particularly where resources limited. In this study based on in silico modeling predictions made with Autodock Vina, the binding affinity of an optimized novel peptide (Pf_P1: KITTTDEEVEGIFD) was altered compared that original epitope (P1: KITDEEVEGIFDC). The energy Pf_P1 implies it has stronger interactions...
Currently, the point of care testing (POCT) is not fully developed for subtype-specific avian influenza virus detection. In this study, an H5N1 hemaglutinin 1 (HA1) epitope (P0: KPNDAINF) and three modified peptides (P1: KPNTAINF, P2: KPNGAINF, P3: KPNDAINDAINF) were evaluated as POCT elements rapid detection virus. Based on modeling predictions by Autodock Vina, binding affinity varied depending alteration one amino acid in these peptides. The energy P2 indicated its potential a strong...
The highly pathogenic avian influenza H5 2.3.4.4 and 2.3.2.1c subclades have distinct antigenic properties are responsible for the majority of human infections. Therefore, it is essential to understand processes by which antibodies inhibit these subclade viruses develop effective therapies vaccines prevent their escape from neutralizing antibodies. Herein, we report epitopes two specific monoclonal (mAbs) targeting haemagglutinin (HA) 2.3.4.4b abilities. results indicated that mAbs provided...
Several novel highly pathogenic avian influenza (HPAIVs) A(H5N6) viruses were reported in Mongolia 2020, some of which included host-specific markers associated with mammalian infection. However, their pathogenicity has not yet been investigated. Here, we isolated and evaluate two genotypes subtype during 2018-2019 (A/wildDuck/MN/H5N6/2018-19). Their evolution pattern molecular characteristics evaluated using gene sequencing was determined a mouse model. We also compared antigenicity...
Rapid diagnosis is essential for the control and prevention of H5 highly pathogenic avian influenza viruses (HPAIVs). However, sensitive rapid diagnostic systems have shown limited performance due to specific antibody scarcity. In this study, two novel monoclonal antibodies (mAbs) clade 2.3.4.4 H5Nx were developed by using an immunogen from a reversed genetic virus (RGV). These mAbs combined with fluorescence europium nanoparticles optimized lysis buffer, which further used developing...
Influenza A virus subtype H1N1 has caused global pandemics like the “Spanish flu” in 1918 and 2009 pandemic several times. remains circulation survives multiple animal sources, including wild birds. Surveillance during winter of 2018–2019 Korea revealed two isolates samples collected from bird feces: KNU18-64 (A/Greater white-fronted goose/South Korea/KNU18-64/2018(H1N1)) WKU19-4 (A/wild bird/South Korea/WKU19-4/2019(H1N1)). Phylogenetic analysis indicated that M gene KNU18-64(H1N1) isolate...
Low-pathogenicity avian influenza viruses (LPAIV) introduced by migratory birds circulate in wild and can be transmitted to poultry. These mutate become highly pathogenic causing severe disease death In March 2019, an H7N3 virus-A/Spot-billed duck/South Korea/WKU2019-1/2019 (H7N3)-was isolated from spot-billed ducks South Korea. This study aimed evaluate the phylogenetic mutational analysis of this isolate. Molecular revealed that genes for HA (hemagglutinin) NA (neuraminidase) strain...
The avian influenza (AI) virus causes a highly contagious disease which is common in wild and domestic birds sporadic humans. Mutations genetic reassortments among the 8 negative-sense RNA segments of viral genome alter its pathogenic potential, demanding well-targeted, active surveillance for infection control.Wild duck fecal samples were collected during 2018 bird health annual South Korea tracking variations AI virus. One low-pathogenic H5N3 reassortment (A/mallard duck/South...
Avian influenza virus (AIV) subtypes H5 and H7, possessing the ability to mutate spontaneously from low pathogenic (LP) highly (HP) variants, are major concerns for enormous socio-economic losses in poultry industry, as well fatal human infections. Through antigenic drift shift, genetic reassortments of genotypes pose serious threats increased virulence pathogenicity leading potential pandemics. In this study, we isolated H7-subtype AIVs circulating Republic Korea during 2018-2019, perform...
Influenza, a highly contagious respiratory infectious disease caused by an influenza virus, is threat to public health worldwide. Avian viruses (AIVs) have the potential cause next pandemic crossing species barrier through mutation of viral genome. Here, we investigated pathogenicity AIVs obtained from South Korea and Mongolia during 2018-2019 measuring titers in lungs extrapulmonary organs mouse models. In addition, assessed ferret Moreover, compared ability replicate mammalian cells, as...