A5076359899- Fungal and yeast genetics research
- Antifungal resistance and susceptibility
- Biofuel production and bioconversion
- Microtubule and mitosis dynamics
- Polysaccharides and Plant Cell Walls
- Enzyme Production and Characterization
- Plant-Microbe Interactions and Immunity
- Genomics and Chromatin Dynamics
- Fungal Infections and Studies
- Mycotoxins in Agriculture and Food
- Polyamine Metabolism and Applications
- Wnt/β-catenin signaling in development and cancer
- Bacterial Genetics and Biotechnology
- Studies on Chitinases and Chitosanases
- Microbial Metabolites in Food Biotechnology
- RNA Research and Splicing
- Retinoids in leukemia and cellular processes
- RNA regulation and disease
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Proteins in Food Systems
- Electrospun Nanofibers in Biomedical Applications
- Axial and Atropisomeric Chirality Synthesis
- Transgenic Plants and Applications
- DNA Repair Mechanisms
Universidad de Extremadura
2006-2023
Texas State University
2013-2015
The Honourable Society of Lincoln's Inn
1994-1997
Universidad de Salamanca
1990-1993
Consejo Superior de Investigaciones Científicas
1993
National Institute of Diabetes and Digestive and Kidney Diseases
1982
Laboratoire de Biochimie
1982
Institute of Art
1982
National Institutes of Health
1982
The ability of Candida albicans to switch cellular morphologies is crucial for its cause infection. Because the cell cycle machinery participates in Saccharomyces cerevisiae filamentous growth, we characterized detail two C. B-type cyclins, CLB2 and CLB4, better understand molecular mechanisms that underlie morphogenic switch. Both Clb2p Clb4p levels are regulated, peaking at G2/M declining before mitotic exit. On hyphal induction, accumulation G1 cyclin Cln1p was prolonged, whereas both Clb...
Wild t.ype refers here to the structure of mannan.Thus, strain
Many types of tumor, including glioma, melanoma, non-small cell lung, esophageal, and head neck cancer, among others, are intrinsically resistant to apoptosis induction poorly responsive current therapies with proapoptotic agents. In addition, tumors often develop multidrug resistance based on the cellular efflux chemotherapeutic Thus, novel anticancer agents capable overcoming these intrinsic or developed tumor mechanisms urgently needed. We describe a series...
When Candida albicans yeast cells receive the appropriate stimulus, they switch to hyphal growth, characterized by continuous apical elongation and inhibition of cell separation. The molecular basis this is poorly known, despite its crucial importance for development. In C. albicans, septins are important hypha formation virulence. Here, we used fluorescence recovery after photobleaching analysis characterize dynamics septin rings during growth. On induction, converted a hyphal-specific...
During the conversion of yeast cells to protoplasts, about half chitinase was liberated into medium, an indication that this portion enzyme is located in periplasmic space. At least part remaining appears be enclosed vacuoles or vesicle co-purify with them, as indicated by a 14-fold enrichment enzymatic activity vacuole fraction isolated from protoplast lysate. When protoplasts were incubated growth medium. It concluded secretory enzyme, like invertase and acid phosphatase. The stored...
We have characterized the CDC14 gene, which encodes a dual-specificity protein phosphatase in Candida albicans, and demonstrated that its deletion results defects cell separation, mitotic exit morphogenesis. The C. albicans cdc14Δ mutants formed large aggregates of cells resembled those found ace2-null strains. In cells, expression Ace2p target genes was reduced did not accumulate specifically daughter nuclei. Taken together, these imply Cdc14p is required for activation daughter-specific...
Nuclear Dbf2-related (NDR) protein kinases are essential components of regulatory pathways involved in cell morphogenesis, cycle control, and viability eukaryotic cells. For their activity function, these require interaction with Mob proteins. However, little is known about how the proteins regulated. In Candida albicans, cyclin-dependent kinase (CDK) Cdc28 NDR Cbk1 required for hyphal growth. Here we demonstrate that Mob2, activator, undergoes a Cdc28-dependent differential phosphorylation...
In nature, many microorganisms form specialized complex, multicellular, surface-attached communities called biofilms. These play critical roles in microbial pathogenesis. The fungal pathogen Candida albicans is associated with catheter-based infections due to its ability establish transcription factor Bcr1 a master regulator of C. biofilm development, although the full extent regulation remains unknown. Here, we report that phosphoprotein physically interacts NDR kinase Cbk1 and undergoes...
Paxillin is a scaffold protein that participates in focal adhesion signaling mammalian cells. Fission yeast paxillin ortholog, Pxl1, required for contractile actomyosin ring (CAR) integrity and collaborates with the β-glucan synthase Bgs1 septum formation. We show here Pxl1's main function to recruit calcineurin (CN) phosphatase ring; thus absence of either Pxl1 or causes similar cytokinesis defects. In turn, CN dephosphorylation Cdc15 F-BAR protein, which recruits concentrates at CAR. Our...
Digitonin treatment at 30 degrees C of a Saccharomyces cerevisiae mutant lacking proteinase B permeabilized the cells and caused rapid extensive activation chitin synthetase in situ. The same result was obtained with generally defective vacuolar proteases. By lowering temperature using different permeabilization procedures, we showed that increases permeability are distinct processes. Activation inhibited by protease inhibitors antipain leupeptin, but pepstatin or chymostatin. Metal...
The Cdc14 phosphatase family is highly conserved in fungi. In Saccharomyces cerevisiae, essential for down-regulation of cyclin-dependent kinase activity at mitotic exit. However, this function not broadly and requires only a small fraction normal activity. Here, we identified an invariant motif the disordered C-terminal tail fungal enzymes that required full enzyme Mutation reduced catalytic rate provided tool studying biological significance high A S. cerevisiae strain expressing...
ABSTRACT A variety of inducible protein degradation (IPD) systems have been developed as powerful tools for functional characterization. IPD provide a convenient mechanism rapid inactivation almost any target interest. Auxin-inducible (AID) is one the most common and has established in diverse eukaryotic research model organisms. Thus far, not use pathogenic fungal species. Here, we demonstrate that original AID second generation, AID2, work efficiently rapidly human yeasts, Candida albicans...
In Saccharomyces cerevisiae, the meiotic process is accompanied by a large increase in 1,3-beta-glucan-degradative activity. The molecular cloning of gene (SSG1) encoding sporulation-specific exo-1,3-beta-glucanase was achieved screening genomic library with DNA probe obtained polymerase chain reaction amplification using synthetic oligonucleotides designed according to nucleotide sequence predicted from amino-terminal region purified protein. sequencing indicates that SSG1 specifies...
The synthesis and secretion of a 1,3-1,4-beta-D-glucanase were studied in different strains Escherichia coli transformed with plasmids carrying the Bacillus circulans WL-12 structural gene. This gene (named BGC) is contained within 1.9-kilobase BamHI-HindIII fragment directs E. an enzyme that specifically degrades lichenan. Only one active form was found when expressed strains. electrophoretic pattern this protein showed molecular weight approximately same as mature beta-glucanase secreted...
Journal Article Nucleotide sequence of a 1, 3–1, 4-β-glucanase-encoding gene in Bacillus circulans WL-12 Get access A. Bueno, Bueno Instituto de Microbiologia-Bioquimica, Facultad Biologia, CSIC/Universidad Salamanca37008 Salamanca, Spain Search for other works by this author on: Oxford Academic PubMed Google Scholar C.R.Vazquez Aldana, Aldana J. Correa, Correa F.del Rey Nucleic Acids Research, Volume 18, Issue 14, 25 July 1990, Page 4248, https://doi.org/10.1093/nar/18.14.4248 Published:...
Candida albicans is a major invasive fungal pathogen in humans. An important virulence factor its ability to switch between the yeast and hyphal forms, these filamentous forms are tissue penetration invasion. A common feature for growth inhibit cell separation after cytokinesis, although it poorly understood how this process regulated developmentally. In C. albicans, formation of filaments during requires changes septin ring dynamics. work, we studied functional relationship septins...
We have characterized the DBF2 gene, encoding a protein kinase of NDR family in Candida albicans, and demonstrate that this gene is essential for cell viability. Conditional mutants were constructed by using MET3 promoter to analyse phenotype cells lacking kinase. The absence Dbf2 resulted arrested as large-budded pairs failed contract actomyosin ring, function similar described its Saccharomyces cerevisiae orthologue. In addition role cytokinesis, regulates mitotic spindle organization...
We have identified the rum1+ gene as a new regulator of G1-phase fission yeast cell cycle. deter-mines cycle timing Start, by maintaining cells in pre-Start state until they attained minimal critical mass. Cells lacking are unable to arrest G1 response nitrogen starvation and subsequently sterile. In addition, prevents entry into mitosis from G1, shown fact that cdclO mutants absence undergo lethal without entering S-phase.
Gene manipulation and epitope tagging are essential tools for understanding the molecular function of specific genes. The opportunistic human pathogen Candida albicans is a diploid fungus that utilizes non-canonical genetic code. Since selection markers available in this organism scarce, several based on recyclable have been developed gene disruption, such as Clox system. This system relies Cre recombinase, which recycles flanked by loxP sites with high efficiency, facilitating single marker...