A5040903381- Fungal and yeast genetics research
- Polysaccharides and Plant Cell Walls
- Biofuel production and bioconversion
- Cellular transport and secretion
- Monoclonal and Polyclonal Antibodies Research
- Microtubule and mitosis dynamics
- Fungal Biology and Applications
- Enzyme Production and Characterization
- Endoplasmic Reticulum Stress and Disease
- Nerve injury and regeneration
- Plant Reproductive Biology
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Protein Kinase Regulation and GTPase Signaling
- Immunotherapy and Immune Responses
- Genetic Neurodegenerative Diseases
- Indigenous Cultures and History
- Receptor Mechanisms and Signaling
- Microbial Metabolites in Food Biotechnology
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Yeasts and Rust Fungi Studies
- Effects and risks of endocrine disrupting chemicals
- Microbial Metabolic Engineering and Bioproduction
Instituto de Biología Funcional y Genómica
2015-2024
Consejo Superior de Investigaciones Científicas
2015-2024
Universidad de Salamanca
2015-2024
Gobierno de Navarra
2022
Instituto de Recursos Naturales y Agrobiología de Salamanca
2019-2021
Universidad Autónoma de Madrid
2020
Charles University
2020
Universidad Nacional de Colombia
2019
Duke University
2016
Hôpital d'instruction des Armées Desgenettes
2013-2015
We tested some resin-based composites used in dentistry for their estrogenic activity. A sealant based on bisphenol-A diglycidylether methacrylate (bis-GMA) increased cell yields, progesterone receptor expression, and pS2 secretion human estrogen-target, serum-sensitive MCF7 breast cancer cells. Estrogenicity was due to dimethacrylate, monomers found the base paste of dental identified by mass spectrometry. Samples saliva from 18 subjects treated with 50 mg a bis-GMA-based applied molars...
A new lignin-degrading basidiomycete, strain PM1 (= CECT 2971), was isolated from the wastewater of a paper factory. The major ligninolytic activity detected in basidiomycete culture supernatant phenoloxidase (laccase). This produced constitutively defined or complex media and appeared as two protein bands native gel electrophoresis preparations. No enzyme induction found after treatment with certain potential laccase inducers. Laccase I purified to homogeneity by filtration chromatography,...
The chemical structure of hydroxylated diphenylalkanes or bisphenols consists two phenolic rings joined together through a bridging carbon. This class endocrine disruptors that mimic estrogens is widely used in industry, particularly plastics. Bisphenol F, bisphenol A, fluorine-containing A (bisphenol AF), and other were found to be estrogenic bioassay with MCF7 human breast cancer cells culture (E-SCREEN assay). Bisphenols promoted cell proliferation increased the synthesis secretion...
Ligand-induced receptor oligomerization is an established mechanism for receptor-tyrosine kinase activation. However, numerous kinases are expressed in multicomponent complexes with other receptors that may signal independently or alter the binding characteristics of kinase. Nerve growth factor (NGF) interacts two structurally unrelated receptors, Trk A and p75, a tumor necrosis family member. Each binds to NGF predominantly low affinity (K(d) = 10(-9) m), but they produce high sites 10(-11)...
Formins are conserved actin nucleators responsible for the assembly of diverse structures. Many formins controlled through an autoinhibitory mechanism involving interaction a C-terminal DAD sequence with N-terminal DID sequence. Here, we show that fission yeast formin for3p, which mediates cable and polarized cell growth, is regulated by similar in vivo. Multiple sites govern for3p localization to tips. The activity inhibited intramolecular divergent DID-like sequences. A mutant expressed at...
Nerve growth factor (NGF) initiates its trophic effects by longrange signaling through binding, internalization, and transport of a ligand-receptor complex from the axon terminal to cell body.However, mechanism which retrograde NGF takes place has not been elucidated.Here we describe an interaction between Trk receptor tyrosine kinase 14 kDa light chain cytoplasmic dynein.After transfection in human embryonic kidney 293 cells, this dynein was found bind TrkA, TrkB, TrkC receptors.Mapping...
Using a hierarchical approach, 620 non-essential single-gene yeast deletants generated by EUROFAN I were systematically screened for cell-wall-related phenotypes. By analyzing altered sensitivity to the presence of Calcofluor white or SDS in growth medium, sonication, abnormal morphology, 145 (23%) mutants showing at least one cell wall-related phenotype selected. These further identify genes potentially involved either biosynthesis, remodeling coupling wall macromolecules overall regulation...
In fission yeast protein kinase C homologues (Pck1 and Pck2) are essential for cell morphogenesis. We have isolated mok1(+) in a genetic screen to identify downstream effectors Pck1/2. is viability encodes that has several membrane-spanning domains regions homologous glucan metabolic enzymes. mok1 mutant shows abnormal shape, randomization of F-actin weak wall. Biochemical analysis Mok1 appears alpha-glucan synthase activity. localization undergoes dramatic alteration during the cycle. It...
We have induced fresh peripheral blood K/natural killer cells to lyse a variety of target by coating them with anti-Fc gamma receptor (anti-Fc R) (CD16) antibody hetero-cross-linked anti-target cell antibody. The cytotoxic mediating this activity is different, as judged depletion studies, from the CD3+, CD8+ T which targeted anti-CD3 cross-linked Targeted K some donors enhanced exposure interleukin 2 but not interferon-gamma; other exhibit high amounts without stimulation. Specificity lysis...
We tested some resin-based composites used in dentistry for their estrogenic activity. A sealant based on bisphenol-A diglycidylether methacrylate (bis-GMA) increased cell yields, progesterone receptor expression, and pS2 secretion human estrogen-target, serum-sensitive MCF7 breast cancer cells. Estrogenicity was due to dimethacrylate, monomers found the base paste of dental identified by mass spectrometry. Samples saliva from 18 subjects treated with 50 mg a bis-GMA-based applied molars...
Phytolaccoside B (1), an antifungal monodesmoside triterpenoid glycoside isolated from berries of Phytolacca tetramera Hauman (Phytolaccaceae), alters the morphology yeasts and molds. The malformations were similar to those produced by enfumafungin, a known inhibitor (1→3)-β-d-glucan synthase, enzyme that catalyzes synthesis (1→3)-β-d-glucan, one major polymers fungal cell wall. However, enzymatic assays revealed 1 did not inhibit but it produce notable enhancement chitin synthase activity...
Cytokinesis has been extensively studied in different models, but the role of extracellular cell wall is less understood. Here we this process fission yeast. The essential protein Bgs4 synthesizes main β(1,3)glucan. We show that Bgs4-derived β(1,3)glucan required for correct and stable actomyosin ring positioning middle, before start septum formation anchorage to wall. Consequently, loss generated sliding, oblique positioned rings septa, misdirected synthesis indicative relaxed rings,...
ABSTRACT Schizosaccharomyces pombe rho1 + is required for maintenance of cell integrity and polarization the actin cytoskeleton. However, no other effector besides (1,3)β-D-glucan synthase enzyme has been identified in S. pombe. We have further investigated if rho1+ signalling could be also mediated by two protein kinase C homologues, pck1p pck2p. show this study that both kinases interact with rho1p rho2p only when bound to GTP, as most GTPase effectors do. Interestingly, interaction was...
Mitogen-activated protein kinase (MAPK) signaling pathways are critical for the sensing and response of eukaryotic cells to extracellular changes. In Schizosaccharomyces pombe, MAPK Pmk1/Spm1 has been involved in cell wall construction, morphogenesis, cytokinesis, ion homeostasis, as part so-called integrity pathway together with Mkh1 Pek1. We show that Pmk1 is activated multiple stress situations, including hyper- or hypotonic stress, glucose deprivation, presence wall-damaging compounds,...
The extracellular region of the nerve growth factor (NGF) receptor, TrkA, contains two immunoglobulin (Ig)-like domains that are required for specific ligand binding. We have investigated possible role these Ig-like in receptor dimerization and activation by using different mutants TrkA region. Deletions each domain, both, entire were made. To probe structural constraints on ligand-independent dimerization, chimeric receptors generated swapping third or fourth domain c-Kit. also introduced...
We have isolated and characterized the cDNA genomic DNA coding for a phenoloxidase, laccase I, previously purified from culture supernatant of newly ligninolytic basidiomycete PM1 (CECT 2971). A library was constructed, laccase-encoding cDNAs were identified by screening with antiserum raised against enzyme. The lac1 gene in partial using as probe. Nucleotide sequence determination full-length revealed an open reading frame 1,551 bp encoding polypeptide 517 amino acid residues putative...
Antibody heteroaggregates have been used to render human peripheral blood T cells lytic for specified targets. The contain anti-T3 covalently linked antibodies against nominal target cell antigens. Such bind directly T3 molecules on effector and trigger lysis. Freshly prepared PBL, when coated with anti-T3-containing heteroaggregates, are without further stimulation, although brief exposure crude lymphokine-containing supernatants or recombinant IL-2, but not IFN-gamma, enhances the...
Schizosaccharomyces pombe thermosensitive mutants requiring the presence of an osmotic stabilizer to survive and grow at a nonpermissive temperature were isolated. The genetically biochemically characterized. In all them, phenotype segregated in Mendelian fashion as single gene which coded for recessive character. Fourteen loci defined by complementation analysis. Studies cell wall composition showed reduction amount beta-glucan three strains (JCR1, JCR5, JCR10) when growing 37 degrees C....
Background An excess of caffeine is cytotoxic to all eukaryotic cell types. We aim study how cells become tolerant a toxic dose this drug, and the relationship between oxidative stress pathways. Methodology/Principal Findings searched for Schizosaccharomyces pombe mutants with inhibited growth on caffeine-containing plates. screened collection 2,700 haploid mutant cells, which 98 were sensitive caffeine. The genes mutated in these clones involved number cellular roles including H2O2-induced...