A5042660745- Fungal and yeast genetics research
- Fermentation and Sensory Analysis
- Yeasts and Rust Fungi Studies
- Horticultural and Viticultural Research
- Plant Pathogens and Fungal Diseases
- Biofuel production and bioconversion
- Fungal Biology and Applications
- Mycorrhizal Fungi and Plant Interactions
- Plant Reproductive Biology
- Microbial Metabolic Engineering and Bioproduction
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Antifungal resistance and susceptibility
- DNA Repair Mechanisms
- Plant-Microbe Interactions and Immunity
- RNA Research and Splicing
- Fungal Infections and Studies
- Polysaccharides and Plant Cell Walls
- Plant Disease Resistance and Genetics
- Bacterial Genetics and Biotechnology
- Wine Industry and Tourism
- Plant nutrient uptake and metabolism
- RNA and protein synthesis mechanisms
- Fungal Plant Pathogen Control
- Polyamine Metabolism and Applications
University of Debrecen
2016-2025
Hungarian Academy of Sciences
2000-2007
Heidelberg University
2005
Weatherford College
2001
University of Basilicata
2001
Chiba University
1998
Centre de Génétique Moléculaire
1994
KLE University
1990-1991
University of Bern
1986
University of Edinburgh
1983
Noble-rotted grapes are colonized by complex microbial populations. I isolated pigment-producing Metschnikowia strains from noble-rotted that had antagonistic activity against filamentous fungi, yeasts, and bacteria. A red-maroon pigment was formed a diffusible colorless precursor released the cells into medium. The conversion of required iron could occur both in (red colonies) medium halos around colonies). intensity pigmentation correlated with antimicrobial activity. Mutants did not form...
Abstract The fission yeast Schizosaccharomyces pombe does not form tripartite synaptonemal complexes during meiotic prophase, but axial core-like structures (linear elements). To probe the relationship between recombination and structure, pairing, segregation of chromosomes, we genetically cytologically characterized rec8-110 mutant, which is partially deficient in recombination. pattern spore viability indicates that chromosome affected mutant. A detailed segregational analysis mutant...
The yeast Candida zemplinina (Starmerella bacillaris) is frequently isolated from grape and wine environments. Its enological use in mixed fermentation with Saccharomyces cerevisiae has been extensively investigated these last few years, several interesting features including low ethanol production, fructophily, glycerol other metabolites have described. In addition, molecular tools allowing the characterization of populations developed, both at inter- intraspecific levels. However, most...
Schizosaccharomyces pombe cells divide by medial fission throughout contraction of an actomyosin ring and deposition a multilayered division septum that must be cleaved to release the two daughter cells. Although many studies have focused on actomoysin assembly,little information is available concerning mechanism cell separation. Here we describe characterization eng1+, new gene encodes protein with detectable endo-β-1,3-glucanase activity whose deletion not lethal but does interfere in...
Four yeast strains isolated from fermenting botrytized grape musts in the Tokaj wine region of Hungary are shown to represent a new osmotolerant and psychrotolerant species. The species, Candida zemplinina (type strain 10-372T=CBS 9494T=NCAIM Y016667T), is closely related stellata, common on overripe grapes sweet wines. sequence D1/D2 domain C. 10-372T 26S rDNA shows 8·1 % difference when compared its counterpart stellata CBS 157T. In conserved 5·8S gene ITS1–5·8S–ITS2 8 %. differs only at...
We characterized a number of widely used yeast-Escherichia coli shuttle vectors in the fission yeast Schizosaccharomyces pombe. The 2 micron pDB248 and YEp13 showed high frequency transformation, intermediate mitotic low meiotic stability, copy S. pombe, analogous to their behavior [cir0] strains Saccharomyces cerevisiae. cerevisiae integration pLEU2 pURA3 transformed pombe at very frequencies but, surprisingly, nonintegrative fashion. Instead, they replicated autonomously, numbers (up 150...
Cytokinesis has been extensively studied in different models, but the role of extracellular cell wall is less understood. Here we this process fission yeast. The essential protein Bgs4 synthesizes main β(1,3)glucan. We show that Bgs4-derived β(1,3)glucan required for correct and stable actomyosin ring positioning middle, before start septum formation anchorage to wall. Consequently, loss generated sliding, oblique positioned rings septa, misdirected synthesis indicative relaxed rings,...
The conversion of grape must into wine involves the development and succession yeast populations differing in species composition. initial population is formed by vineyard strains which are washed from crushed grapes then completed with yeasts coming cellar environment. As origin natural habitat not fully understood, this study addresses possibility that can be preserved berries left behind on vines at harvest until spring next year. These become mummified during winter vines. To investigate...
Summary Protein O‐mannosyltransferases (PMTs) initiate the assembly of O‐mannosyl glycans, which are fundamental importance in eukaryotes. The PMT family, is classified into PMT1, PMT2 and PMT4 subfamilies, evolutionarily conserved. Despite fact that PMTs crucial for viability baker's yeast as well mouse, recent studies suggested there significant differences organization properties O‐mannosylation machinery between yeasts mammals. In this study we identified characterized family...
Yeasts identified as Candida stellata are frequently associated with overripe and botrytized grapes can survive in the fermenting must until completion of vinification. The molecular taxonomic examination 41 strains deposited six culture collections or described literature C. revealed that most those isolated from wines belonged to zemplinina related species. This confusion around position may account for rather controversial descriptions oenological properties literature. Because authors...
Abstract Two strains of fission yeast were isolated from honey. They differ the type strain Schizosaccharomyces octosporus by three substitutions in D1/D2 domain nuclear 26S large subunit ribosomal RNA (rRNA) gene sequence, resulting a 99.5% identity. In internal transcribed spacer (ITS) region (consisting ITS1, 5.8S rDNA, and ITS2), S. 16 gaps 91 substitutions, which is equivalent to an identity 88.1%. Genome sequencing on one new revealed that average nucleotide (ANI) between its genome...
Yeast strains representing a novel asexual ascomycetous species were isolated from seven Malva sylvestris flowers. Sequencing of the chromosomal regions coding for D1/D2 domains large subunit ribosomal RNA, ITS1-5.8S-ITS2 segments and parts gene small RNA showed that isolates conspecific. Comparative analysis these sequences corresponding type yeasts revealed represent hitherto undescribed belonging to sensu stricto subclade genus Starmerella . The new is osmotolerant can develop invasive...
Schizosaccharomyces pombe cells divide by medial fission through contraction of an actomyosin ring and deposition a multilayered division septum that must be cleaved to release the two daughter cells. Here we describe identification seven genes (adg1(+), adg2(+), adg3(+), cfh4(+), agn1(+), eng1(+), mid2(+)) whose expression is induced transcription factor Ace2p. The all these varied during cell cycle, maximum being observed septation. At least three proteins (Eng1p, Agn1p, Cfh4p) localize...
In a taxonomic study of yeasts isolated from flowers in Cagayan de Oro, Mindenao Island, The Philippines, strains were identified as representing Kabatiella microsticta, Metschnikowia koreensis and hitherto undescribed dimorphic species. Sequences the D1/D2 domains LSU 26S rRNA genes, internal transcribed spacer (ITS) regions SSU 18S genes identical last-named group differed corresponding sequences type strain closest related species, Candida duobushaemulonii, by 4 % (D1/D2), 7 1 (SSU). an...