A5032527597- Fungal and yeast genetics research
- Polysaccharides and Plant Cell Walls
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Mitochondrial Function and Pathology
- Machine Learning in Bioinformatics
- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- Neurological Disease Mechanisms and Treatments
- Glycosylation and Glycoproteins Research
- Genetic Neurodegenerative Diseases
- Mechanisms of cancer metastasis
- Fermentation and Sensory Analysis
- Plant Reproductive Biology
- Caveolin-1 and cellular processes
Consejo Superior de Investigaciones Científicas
2010-2019
Universidad de Salamanca
2009-2019
Instituto de Biología Funcional y Genómica
2012-2019
Fungal cytokinesis requires the assembly of a dividing septum wall. In yeast, has to be selectively digested during critical cell separation process. Fission yeast wall α(1-3)glucan is essential, but nothing known about its localization and function in or cooperation between α- β(1-3)glucan synthases Ags1 Bgs for assembly. Here, we generate physiological Ags1-GFP variant demonstrate tight colocalization with Bgs1, suggesting important early steps construction. Moreover, define essential...
Cytokinesis has been extensively studied in different models, but the role of extracellular cell wall is less understood. Here we this process fission yeast. The essential protein Bgs4 synthesizes main β(1,3)glucan. We show that Bgs4-derived β(1,3)glucan required for correct and stable actomyosin ring positioning middle, before start septum formation anchorage to wall. Consequently, loss generated sliding, oblique positioned rings septa, misdirected synthesis indicative relaxed rings,...
Three specific β(1,3)glucan synthase (GS) inhibitor families, papulacandins, acidic terpenoids, and echinocandins, have been analyzed in Schizosaccharomyces pombe wild-type papulacandin-resistant cells GS activities. Papulacandin enfumafungin produced similar vivo effects, different from that of echinocandins. Also, papulacandin was the strongest vitro (IC50 103–104-fold lower than with or pneumocandin), but caspofungin by far most efficient antifungal because following. 1) It only drug...
In fission yeast, cytokinesis requires a contractile actomyosin ring (CR) coupled to membrane and septum ingression. Septation proceeds in two phases. anaphase B, the ingresses slowly. During telophase, ingression rate increases, CR becomes dispensable. Here, we explore relationship between septation by analyzing ultrastructure, ingression, proteins cells lacking F-actin. We show that phases of correlate with maturation response F-actin removal. first phase, is immature and, following...
In fungi, success of mating requires that both cells agglutinate, modify their extracellular envelopes, and fuse plasma membranes nuclei to produce a zygote. Here we studied the role Schizosaccharomyces pombe Dni1 protein in cell fusion step mating. Dni1p is tetraspan bearing conserved cystein motif similar present fungal claudin-related proteins. expression induced during concentrates as discrete patches at cell-cell contact area along bridge. Proper localization depends on Fus1p, actin...