A5016043736- Fungal and yeast genetics research
- Genetics, Aging, and Longevity in Model Organisms
- Biofuel production and bioconversion
- Microtubule and mitosis dynamics
- Endoplasmic Reticulum Stress and Disease
- Fermentation and Sensory Analysis
- Protein Kinase Regulation and GTPase Signaling
- Cellular transport and secretion
- Polysaccharides and Plant Cell Walls
- Microbial Metabolic Engineering and Bioproduction
- Heat shock proteins research
- Microbial Inactivation Methods
- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- Fungal Biology and Applications
- Antifungal resistance and susceptibility
- Enzyme-mediated dye degradation
- Plant Reproductive Biology
- Spaceflight effects on biology
- Enzyme Structure and Function
- Bacillus and Francisella bacterial research
- Plant Gene Expression Analysis
- Bacterial Genetics and Biotechnology
- Genetic Neurodegenerative Diseases
- Autophagy in Disease and Therapy
Universidad de Murcia
2013-2023
Complejo Hospitalario de Pontevedra
2013
Universidad Complutense de Madrid
1994
The Schizosaccharomyces pombe stress-activated Sty1p/Spc1p mitogen-activated protein (MAP) kinase regulates gene expression through the Atf1p and Pap1p transcription factors, homologs of human ATF2 c-Jun, respectively. Mcs4p, a response regulator protein, acts upstream Sty1p by binding Wak1p/Wis4p MAP kinase. We show that phosphorylation Mcs4p on conserved aspartic acid residue is required for activation only in to peroxide stress. phospho-relay system initiated two PAS/PAC domain-containing...
Mitogen-activated protein kinase (MAPK) signaling pathways are critical for the sensing and response of eukaryotic cells to extracellular changes. In Schizosaccharomyces pombe, MAPK Pmk1/Spm1 has been involved in cell wall construction, morphogenesis, cytokinesis, ion homeostasis, as part so-called integrity pathway together with Mkh1 Pek1. We show that Pmk1 is activated multiple stress situations, including hyper- or hypotonic stress, glucose deprivation, presence wall-damaging compounds,...
Recent studies have shown that heat shock proteins and trehalose synthesis are important factors in the thermotolerance of fission yeast Schizosaccharomyces pombe. We examined effects trehalose-6-phosphate (trehalose-6P) synthase overexpression on resistance to several stresses cells S. pombe transformed with a plasmid bearing tps1 gene, which codes for trehalose-6P synthase, under control strong thiamine-repressible promoter. Upon induction elevated levels intracellular correlated not only...
Abstract In eukaryotic cells, the highly conserved Target of Rapamycin (TOR) and Mitogen Activated Protein Kinase (MAPK) signaling pathways elicit adaptive responses to extra- intracellular conditions by regulating essential cellular functions. However, nature functional relationships between both is not fully understood. fission yeast Schizosaccharomyces pombe cell integrity MAPK pathway (CIP) regulates morphogenesis, wall structure ionic homeostasis. We show that Rab GTPase Ryh1, a TORC2...
In Schizosaccharomyces pombe, glucose concentrations below a certain threshold trigger the stress-activated protein kinase (SAPK) signal transduction pathway and promote increased transcription of Atf1-dependent genes coding for general stress response. Removal specifically induces nuclear accumulation green fluorescent protein-labeled Pap1 (GFP-Pap1) expression dependent on this factor. contrast, depletion nitrogen source triggers SAPK but does not activate Pap1-dependent gene...
In the fission yeast Schizosaccharomyces pombe cell integrity pathway (CIP) orchestrates multiple biological processes like wall maintenance and ionic homeostasis by fine tuning activation of MAPK Pmk1 in response to various environmental conditions. The small GTPase Rho2 positively regulates CIP through protein kinase C ortholog Pck2. However, retains some function mutants lacking either or Pck2, suggesting existence additional upstream regulatory elements modulate its activity depending on...
Quorum sensing (QS), a mechanism of microbial communication dependent on cell density, governs developmental decisions in many bacteria and some pathogenic non-pathogenic fungi including yeasts. In these simple eukaryotes this response is mediated by the release into growth medium quorum-sensing molecules (QSMs) whose concentration increases proportionally to population density. To date occurrence QS restricted few yeast species. We show that aromatic alcohols phenylethanol tryptophol...
Fission yeast mitogen-activated protein kinase (MAPK) Pmk1p is involved in morphogenesis, cytokinesis, and ion homeostasis as part of the cell integrity pathway, it becomes activated under multiple stresses, including hyper- or hypotonic conditions, glucose deprivation, wall-damaging compounds, oxidative stress. The only phosphatase known to dephosphorylate inactivate Pmp1p. We show here that stress-activated (SAPK) pathway its main effector, Sty1p MAPK, are essential for proper deactivation...
The receptor of activated C kinase (RACK1) is a protein highly conserved among eukaryotes. In mammalian cells, RACK1 functions as an adaptor to favor (PKC)-mediated phosphorylation and subsequent activation c-Jun NH(2)-terminal mitogen-activated kinase. Cpc2, the orthologue in fission yeast Schizosaccharomyces pombe, involved control G2/M transition interacts with Pck2, PKC-type member cell integrity Pmk1 (MAPK) pathway. Both Cpc2 are structural components 40S ribosomal subunit, recent data...
Rho GTPase-activating proteins (GAPs) are responsible for the inactivation of GTPases, which involved in regulation critical biological responses eukaryotic cells, ranging from cell cycle control to cellular morphogenesis. The genome fission yeast Schizosaccharomyces pombe contains six genes coding putative whereas nine code predicted GAPs (Rga1 Rga9). One them, Rga4, has been recently described as a Cdc42 GAP, diameter and symmetry yeast. In this work we show that Rga4 is also Rho2 GAP...
In the fission yeast Schizosaccharomyces pombe Wak1p/Win1p‐Wis1p‐Sty1p stress‐activated protein kinase (SAPK) pathway relays environmental signals to transcriptional machinery and modulates gene expression via a cascade of phosphorylation. Cells S. subjected cold shock (transfer from 28 °C 15 °C) transiently activated Sty1p mitogen‐activated (MAPK) by Induction this response was completely abolished in cells disrupted upstream regulator Mcs4p. The cold‐triggered activation partially...
Rho1 GTPase is the main activator of cell wall glucan biosynthesis and regulates actin cytoskeleton in fungi, including Schizosaccharomyces pombe. We have obtained a fission yeast thermosensitive mutant strain carrying rho1-596 allele, which displays reduced activity. This has severe defects growth, partially suppressed by osmotic stabilization. In global screening for multicopy suppresors pmp1+ gene was identified. Pmp1 dual specificity phosphatase that negatively Pmk1 mitogen-activated...
The fission yeast small GTPase Rho2 regulates morphogenesis and is an upstream activator of the cell integrity pathway, whose key element, mitogen-activated protein kinase (MAPK) Pmk1, becomes activated by multiple environmental stimuli controls several cellular functions. Here we demonstrate that farnesylated palmitoylated in vivo at cysteine-196 within its carboxyl end this modification allows specific targeting to plasma membrane. Unlike other prenylated GTPases, control Pmk1 activity...
Cytokinesis, the separation of daughter cells at end mitosis, relies in animal on a contractile actomyosin ring (CAR) composed actin and class II myosins, whose activity is strongly influenced by regulatory light chain (RLC) phosphorylation. However, simple eukaryotes such as fission yeast Schizosaccharomyces pombe , RLC phosphorylation appears dispensable for regulating CAR dynamics. We found that redundant Ser35 S. homolog Rlc1 p21-activated kinases Pak1 Pak2, modulates myosin Myo2 becomes...
Cell morphogenesis is a complex process that depends on cytoskeleton and membrane organization, intracellular signalling vesicular trafficking. The rod shape of the fission yeast Schizosaccharomyces pombe availability powerful genetic tools make this species an excellent model to study cell morphology. Here we have investigated function conserved Kin1 kinase. Kin1-GFP associates dynamically with plasma at sites active surface remodelling present in fraction. Kin1Δ null cells show severe...
Glucose is a signaling molecule which regulates multiple events in eukaryotic organisms and the most preferred carbon source fission yeast Schizosaccharomyces pombe. The ability of this to grow absence glucose becomes strongly limited due lack enzymes glyoxylate cycle that support diauxic growth. stress-activated protein kinase (SAPK) pathway its effectors, Sty1 MAPK transcription factor Atf1, play critical role adaptation on alternative non-fermentable sources by inducing expression fbp1 +...
Macroautophagy/autophagy is an essential adaptive physiological response in eukaryotes induced during nutrient starvation, including glucose, the primary immediate carbon and energy source for most cells. Although molecular mechanisms that induce autophagy glucose starvation have been extensively explored budding yeast Saccharomyces cerevisiae, little known about how this coping regulated evolutionary distant fission Schizosaccharomyces pombe. Here, we show S. pombe to limitation relies on...
Centrifugation of cells Schizosaccharomyces pombe in liquid medium prompted a marked activation Sty1 and Pmk1, which are the effector mitogen-activated protein kinases (MAPKs) stress-activated kinase pathway cell-integrity pathway, respectively. Transduction centrifugation signals showed sensitivity threshold above response was dependent on time temperature. Centrifugation-induced phosphorylation Pmk1 required presence main functional components respective signalling cascades, i.e. Wak1 or...
Fission yeast PKC ortholog Pck2 controls cell wall synthesis and is a major upstream activator of the integrity pathway (CIP) its core component, MAP kinase Pmk1, in response to environmental stimuli. We show that vivo phosphorylation at conserved T842 activation loop during growth different stresses mediated by PDK Ksg1 an autophosphorylation mechanism. However, not essential for Pmk1 activation, putative T846 might play additional role catalytic downstream signaling. These events together...
Cytokinesis, which enables the physical separation of daughter cells once mitosis has been completed, is executed in fungal and animal by a contractile actin- myosin-based ring (CAR). In fission yeast Schizosaccharomyces pombe, formin For3 nucleates actin cables also co-operates for CAR assembly during cytokinesis. Mitogen-activated protein kinases (MAPKs) regulate essential adaptive responses eukaryotic organisms to environmental changes. We show that stress-activated kinase pathway (SAPK)...