Nepetanudoside B (NNB) was isolated from aerial parts of endemic Nepeta aristata crude extract (CH3OH-CHCl3) using silica gel (n-hexane, methanol, ethyl acetate, and dichlorometane, respectively) sephadex LH-20 (65% Methanol-35% Chloroform) column chromatographies. Preparative-HPLC used to purify NNB after activity-guided isolation methanol sub-fractions with enzyme inhibitory DNA protective properties. The determined 1H,13C, COSY, HSQC, HMBC, LC-MS/MS. study compared the effects...

Ursolic acid (UA), which has many biological properties such as anti-cancer, anti-inflammatory and antioxidant, regulates some pharmacological processes, been isolated from the flowers, leaves, berries fruits of plant species. In this work, UA was purified methanol-chloroform crude extract Nepeta species (N. aristata, N. baytopii, italica, trachonitica, stenantha) using a silica gel column with chloroform or ethyl acetate solvents via bioactivity-guided isolation. The most active...

1,5,9-epideoxyloganic acid (ELA) was isolated from the aerial parts of endemic

Parietin was isolated from Xanthoria parietina (L.) Th. Fr.' (methanol:chloroform) extract, using a silica column. 13 C NMR and 1H were used to confirm the structure of parietin. For first time, parietin investigated for its antioxidant, antibacterial DNA protective activities. Molecular docking carried out determine binding affinity interactions between enzymes our molecule. Inhibition kinetic mechanism studies action performed too. exhibited high metal chelating activity. The MIC values...

Abietatrien-3β-ol (ATO) was isolated from the aerial part of Nepeta italica subsp. methanol-chloroform extract and studied antioxidant, enzyme inhibition, DNA protection activities. The plant fractionated by silica gel column chromatography using four different solvents. After active chloroform fraction divided into six subfractions under guidance bioactivity, ATO fourth subfraction. molecular structure determined NMR techniques confirmed with literature data. In antioxidant test, showed...

Atriplex nitens Schkuhr (ANS), which grows naturally in arid and semi-arid regions of the world, is highly resistant to drought salty environments used as food animal feed. This study first performed phytochemical analysis antioxidant urease inhibition activities on obtained methanol crude extract ANS. The catechin isoquercitrin were detected main compounds according LC-MS/MS results. Oleic acid methyl ester (31.71%), palmitic (25.87%), linoleic (19.61%), nonacosane (16.81%) GC-MS/MS...

Abstract Several Nepeta species, used in traditional uses, represent sources of valuable phytochemical composition, as well vitro biological and silico clinical activities. In the present study, antioxidant DNA protective properties methanol: chloroform (1 : 1) extracts six species were evaluated by comparison with standards. HPLC‐MS/MS analysis, main components identified rosmarinic acid, caffeic chlorogenic shikimic scutellarin. GC‐FID highest palmitate cis ‐11‐eicosenoic acid. N. aristata...

Due to the common ethnopharmacological used or scientifically examined biochemical properties, Elaeagnaceae family, Elaeagnus umbellate (Thunb.) (EU, Guz yemisi) was worth investigating.In this investigation, we revealed antioxidant, antiproliferative and enzyme inhibition activities of water, methanol, ethanol, acetone, ethyl acetate hexane extracts EU as well contents their phenolic, flavonoid, anthocyanin, ascorbic acid, lycopene β- carotene. The antioxidant activity screened by total...

AbstractIxoroside, an iridoid glycoside, was isolated from two endemic plants, Nepeta aristata and baytopii its structure elucidated by comprehensive analysis with NMR mass spectrophotometers. It observed that the compound exhibited strong antioxidant activity in phosphomolybdenum-reducing power (55.65±0.02 μg/mL), H2O2 (2.97±0.50 superoxide anion scavenging (11.31±0.19 μg/mL) activities. has also been noted ixoroside molecule enzyme inhibitor (7.01±0.001 μg/mL for urease, 19.06±0.51 AChE,...

Abstract The present work aimed to evaluate the phytochemical contents of methanol (ME), ethanol (ET), water (W), acetone (AC), ethyl acetate (EA), and hexane (HE) Aristolochia bodamae root (ABR) as well inhibition potential, antioxidative, antimicrobial activities. chemical composition analysis antioxidant activity ABR extracts were performed by commonly used assays. Antibacterial activities tested against three gram‐positive gram‐negative bacteria using disk diffusion microdilution...

In this study, we investigated the phenolic profile, metal concentrations, and antioxidant antimicrobial activities of edible mushrooms collected from Sinop, Turkey: Amanita caesarea, Boletus edulis, Grifola frondosa, Hydnum repandum, Lactarius deliciosus, L. piperatus, volemus, Laetiporus sulphureus, Pleurotus ostreatus, Ramaria flava. The mycochemical contents R. flava, A. B. edulis were high. cobalt (Co), cadmium (Cd), nickel (Ni), lead (Pb) between < 0.54 8.97 ppm. deliciosus had...

Abstract Nepeta taxa are popular plants and sources of traditional Turkish medicine. Chemical constituents in the essential oils (EOs) hydrodistilled from above‐ground parts aristata (NA), N. baytopii (NB), italica (NI), nuda (NN), stenantha (NS) were determined using GC‐MS. The pharmacological activities EOs, such as biological activities, evaluated vitro . DNA protection NS‐EO found to be most effective. NA‐EO, NI‐EO, more potent urease inhibitors, while exhibited AChE inhibitors. Their...

&lt;p class="Abstract"&gt;This study was designed to evaluate in vitro the total phenolic, flavonoid content, anti-oxidant activity, oxidative DNA-damage protection and anti-cancer activity of n-butanol extract from Perralderia coronopifolia, endemic plant. DNA capacity performed using 46966 plasmid against UV-photolysis H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt;-induced damage. The anti-prolifer-ative effects were on HeLa C6 cells. Experimental results showed that had convenient...

1,5,9-Epideoxyloganic acid (ELA) was isolated from the methanol fraction of endemic Nepeta aristata crude methanol-chloroform by chromatographic methods (preparative-HPLC, silica, and sephadex column). The structure ELA determined with 1H NMR 13C spectrometry. Furthermore, mass molecule confirmed ELA's antioxidant antibacterial activities were examined also compared to standards. exhibited best activity inhibition zone against K. pneumoniae (11.50 mm), S. aureus (10.00 effective minimum...

In this study, chemical content, antioxidant activities, enzyme inhibition and DNA protection activities of extracts obtained from different solvents the Coriandrum sativum leaf, flower seed were determined. Total phenol flavonoid contents C. hexane higher than water, methanol, ethanol ethyl acetate extracts. The highest anthocyanin content was found in part sativum. From activity tests, total aqueous extract, reducing power H2O2 scavenging leaf OH˙ radical free methanol metal chelate...

Various Nepeta species, widely used among the public, have valuable phytochemical contents and clinical biological activities. For this reason, our study examined enzyme inhibition antibacterial properties of methanol: chloroform (1:1) extracts six species. N. aristata showed a higher inhibitory effect than standard drug on seven eight enzymes studied. baytopii had high urease lipase. It was determined that italica inhibited other except for urease, CA, In addition, BChE is also only...

Three furan and/or thiophene-2-carboxamide compounds, namely N-(furan-2-ylmethyl)thiophene-2-carboxamide (1), N-(furan-2-carbonyl)furan-2-carboxamide (2), and N-(Thiophene-2-ylmethyl)furan-2-carboxamide (3) were investigated the enzyme kinetic studies by urease, acetylcholinesterase (AChE), butyrylcholinesterase (BChE). The inhibition constant (Ki) of Compound (CPD)3 AChE was determined as 0.10 mM, Ki value BChE 0.07 mM. In comparison, CPD1 urease These CPDs examined for antioxidant activity...

Paliurus spina-christi Miller (PSC) is a shrub plant with important biological activities. For this reason, the phytochemical and activities of PSC leaf seed extracts were investigated in our study. This study performs analyses (total phenol flavonoid content, LC-ESI-MS/MS, GC-MS/MS) bioactivity assays (antioxidant) for leaves seeds. In silico PASS prediction main compounds LC-ESI-MS/MS GC-MS/MS analysis also investigated. The extract showed high total phenolic content. addition, contents...

Plant-derived bioactive substances have demonstrated significant qualities that suggest they may be crucial in preventing various chronic diseases. Flavonoids, which include apigenin, are the biggest group of polyphenols. In our study, we aimed to obtain methanol-chloroform (1:1) extract from aerial parts

The acid catalyzed hydrolysis of the N-(p-substitutedphenyl) phthalimides in three different acids was investigated at 50.0 ± 0.1 °C. Two antioxidant activity tests as DPPH• and ABTS•+ scavenging activities, various enzyme inhibition urease, acetylcholinesterase (AChE), butyrylcholinesterase (BChE) were applied. Compound 3c (2.03 μg/mL ) has higher than other compounds standards according to DPPH test. In AChE assay, 3a 3b (13.13 9.59 μg/mL) standard Galantamine (14.37 μg/mL). BChE urease...