A5029377932- Advanced Proteomics Techniques and Applications
- Extracellular vesicles in disease
- Bioinformatics and Genomic Networks
- Proteoglycans and glycosaminoglycans research
- S100 Proteins and Annexins
- Bone and Dental Protein Studies
- MicroRNA in disease regulation
- Toxic Organic Pollutants Impact
- Genomics and Rare Diseases
- Connective tissue disorders research
- Bone Metabolism and Diseases
- Thermoregulation and physiological responses
- Body Composition Measurement Techniques
- Physiological and biochemical adaptations
- Coccidia and coccidiosis research
- Bone and Joint Diseases
- Toxoplasma gondii Research Studies
- Biomedical and Engineering Education
- Chronic Myeloid Leukemia Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Monoclonal and Polyclonal Antibodies Research
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Sphingolipid Metabolism and Signaling
Fraunhofer Institute for Cell Therapy and Immunology
2022-2025
Fraunhofer Research Institution for Materials Recycling and Resource Strategies IWKS
2023
Merck (Germany)
2020
Helmholtz Centre for Environmental Research
2014-2019
Our aging population has to deal with the increasing threat of age-related diseases that impair bone healing. One promising therapeutic approach involves coating implants modified glycosaminoglycans (GAGs) mimic native environment and actively facilitate skeletogenesis. In previous studies, we reported coatings containing GAGs, such as hyaluronic acid (HA) its synthetically sulfated derivative (sHA1) well naturally low-sulfated GAG chondroitin sulfate (CS1), reduce activity bone-resorbing...
Prostate cancer (PCa) is the most frequent malignancy in older men with a high propensity for bone metastases. Characteristically, PCa causes osteosclerotic lesions as result of disrupted remodeling. Extracellular vesicles (EVs) participate progression by conditioning pre-metastatic niche. However, how EVs mediate cross-talk between cells and osteoprogenitors microenvironment remains poorly understood. We found that derived from murine cell line RM1-BM increased metabolic activity, vitality,...
Tuberculosis (TB), which is caused by the bacterium Mycobacterium tuberculosis (Mtb), still one of deadliest infectious diseases. Understanding how host and pathogen interact in active TB will have a significant impact on global control efforts. Exosomes are increasingly recognized as means cell-to-cell contact exchange soluble mediators. In case TB, exosomes released from bacillus infected cells. present study, comprehensive lipidomics proteomics analysis size exclusion...
Despite the significant progress in accuracy and reliability mass spectrometry technology, as well development of strategies based on isotopic labeling or internal standards recent decades, systematic biases originating from non-biological factors remain a challenge data analysis. In addition, wide range available normalization methods renders choice suitable method challenging. We systematically evaluated 17 two batch effect correction methods, originally developed for pre-processing DNA...
Abstract Despite the significant progress in accuracy and reliability mass spectrometry technology, as well development of strategies based on isotopic labeling or internal standards recent decades, systematic biases originating from non-biological factors remain a challenge data analysis. In addition, wide range available normalization methods renders choice suitable method challenging. We systematically evaluated 17 2 batch effect correction methods, originally developed for preprocessing...
Bispecific antibodies comprise extremely diverse architectures enabling complex modes of action, such as effector cell recruitment or conditional target modulation via dual targeting, not conveyed by monospecific antibodies. In recent years, research on bispecific therapeutics has substantially grown. However, evaluation binding moiety combinations often leads to undesired prolonged development times. While high throughput screening for small molecules and classical evolved into a mature...
The development of novel bioactive biomaterials is urgently needed to meet the needs an aging population. Both sulfated hyaluronic acid and dexamethasone are candidates for functionalization bone grafts, as they have been shown enhance differentiation osteoblasts from marrow stromal cells in vitro vivo. However, underlying mechanisms not fully understood. Furthermore, studies combining different approaches assess synergistic potentials rare. In this study, we aim gain insights into mode...
Long-term glucocorticoid administration in patients undergoing immunosuppressive and anti-inflammatory treatment is accompanied by impaired bone formation increased fracture risk. Furthermore, can lead to wound healing altered cell metabolism. Recently, we showed that exposure of zebrafish the prednisolone during fin regeneration impacts negatively on length, formation, osteoblast function regenerate. The underlying cellular molecular mechanisms impairment, however, remain incompletely...
Bone healing involves a variety of different cell types and biological processes. Although certain key molecules have been identified, the molecular interactions progress are not completely understood. Moreover, clinical routine for predicting quality bone after fracture in an early phase is missing. This mainly due to lack techniques comprehensively screen cytokines, growth factors metabolites at their local site action. Since all soluble interest present hematoma, its in-depth assessment...
Wound healing of soft tissue and bone defects is a complex process in which cellular differentiation adaption are regulated by internal external factors, among them many different proteins. In contrast to insights into the significance various single proteins based on model systems, knowledge about processes at actual site wound still limited. This caused general lack methods that allow sampling extracellular metabolites, situ . Sampling fluids combination with proteomics metabolomics one...
Introduction The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that regulates broad range of target genes involved in the xenobiotic response, cell cycle control and circadian rhythm. AhR constitutively expressed macrophages (Mϕ), acting as key regulator cytokine production. While proinflammatory cytokines, i.e., IL-1β, IL-6, IL-12, are suppressed through activation, anti-inflammatory IL-10 induced. However, underlying mechanisms those effects importance specific...
Targeting of leukemic stem cells with specific immunotherapy would be an ideal approach for the treatment myeloid malignancies, but suitable epitopes are unknown. The comparative proteome-level characterization hematopoietic and progenitor from healthy cell donors patients acute leukemia has potential to reveal differentially expressed proteins which can used as surface-markers or proxies affected molecular pathways. We employed mass spectrometry methods analyze proteome cytosolic membrane...
Non-healing bone defects are a pressing public health concern accounting for one main cause decreased life expectancy and quality. An aging population accompanied with increasing incidence of comorbidities, foreshadows worsening this socio-economic problem. Conventional treatments non-healing prove ineffective 5%–10% fractures. Those challenges not only increase the patient’s burden but also complicate medical intervention, underscoring need more effective treatment strategies identification...
SUMMARY Monensin (Mon) is an anticoccidial polyether ionophore widely used to control coccidiosis. The extensive use of ionophores on poultry farms resulted in widespread resistance, but the underlying resistance mechanisms are unknown detail. For analysing mode action by which against obtained, we induced vitro Mon Toxoplasma gondii -RH strain (MonR-RH) and compared it with sensitive parental (Sen-RH). proteome assessment MonR-RH Sen-RH strains was obtained after isotopic labelling using...
Proteomics technologies, which include a diverse range of approaches such as mass spectrometry-based, array-based, and others, are key technologies for the identification biomarkers disease mechanisms, referred to mechanotyping. Despite over 15,000 published studies in 2022 alone, leveraging publicly available proteomics data biomarker identification, mechanotyping drug target is not readily possible. Proteomic addressing similar biological/biomedical questions made by multiple research...
Treatment of bone fractures are standardized according to the AO classification, which mainly refers mechanical stabilization required in a given situation but neglect individual differences due patient's healing potential or accompanying diseases. Specially elderly immune-compromised patients, complexity constrains on biological as well level hard account for. Here, we introduce novel framework that allows predict regeneration outcome using combined proteomic and analyses computer model....
Quantitative mass spectrometry has revolutionized proteomics by enabling simultaneous quantification of thousands proteins. Pooling patient-derived data from multiple institutions enhances statistical power but raises significant privacy concerns. Here we introduce FedProt, the first privacy-preserving tool for collaborative differential protein abundance analysis distributed data, which utilizes federated learning and additive secret sharing. In absence a multicenter dataset evaluation,...
Due to multifactorial reasons, such as decreased thirst and total body water, elderly patients are vulnerable dehydration. The study aims investigate whether moderate dehydration or hyperhydration affects the blood proteome. Blood samples, medication, bioelectrical impedance analysis (BIA) details were collected from 131 geriatric (77 women 54 men aged 81.1 ± 7.2 years). Based on an evaluation by Bioelectrical Impedance Vector Analyses (BIVAs) of this cohort, for each hydration status...
<p class="first" dir="auto" id="d14843789e169">Mass spectrometry-based proteomics plays a vital role in biomarker identification and understanding disease mechanisms through mechanotyping. However, utilizing publicly available data for these purposes poses challenges due to diversity involving various sources, model organisms, assay systems. Furthermore, the lack of identifier standardization hinders effective integration across publications. To tackle issues, we introduce ProHarMeD,...