- Forest Insect Ecology and Management
- Plant Pathogens and Fungal Diseases
- Entomopathogenic Microorganisms in Pest Control
- Mycorrhizal Fungi and Plant Interactions
- Nematode management and characterization studies
- Forest Ecology and Biodiversity Studies
- Insect-Plant Interactions and Control
- Yeasts and Rust Fungi Studies
- Insect and Pesticide Research
- Phytochemicals and Antioxidant Activities
- Insect Pest Control Strategies
- Plant-Microbe Interactions and Immunity
- Insect symbiosis and bacterial influences
- Phytochemistry and Biological Activities
- Plant Pathogenic Bacteria Studies
- Allelopathy and phytotoxic interactions
- Insect behavior and control techniques
- Plant Disease Resistance and Genetics
- Date Palm Research Studies
- Natural product bioactivities and synthesis
- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Cholinesterase and Neurodegenerative Diseases
- Insect Resistance and Genetics
- Botany, Ecology, and Taxonomy Studies
Instituto Nacional de Investigação Agrária e Veterinária
2013-2024
University of Lisbon
2017-2020
Ministry of Agriculture, Livestock, and Food Supply
2008
Summary Dothistroma needle blight ( DNB ), caused by septosporum and pini , brown spot BSNB Lecanosticta acicola are some of the most serious damaging foliar diseases pines Pinus spp.). is reported for first time from Ireland Portugal confirmed Black Sea coast Russia (Krasnodar region, Sochi). It has also been recovered first, only, site in Latvia 4 years after efforts to eradicate it were undertaken. on sylvestris radiata . was found Comunitat Valenciana, Spain, as causal agent disease...
Pine Wilt Disease (PWD) has a significant impact on Eurasia pine forests. The microbiome of the nematode (the primary cause disease), its insect vector, and host tree may be relevant for disease mechanism. aim this study was to characterize these microbiomes, from three PWD-affected areas in Portugal, using Denaturing Gradient Gel Electrophoresis, 16S rRNA gene pyrosequencing, functional inference-based approach (PICRUSt). bacterial community structure significantly different infected trees...
Abstract Monochamus beetles are the dispersing vectors of nematode Bursaphelenchus xylophilus , causative agent pine wilt disease (PWD). PWD inflicts significant damages in Eurasian forests. Symbiotic microorganisms have a large influence insect survival. The aim this study was to characterize bacterial community associated Europe and East Asia using culture-independent approach. Twenty-three galloprovincialis were collected Portugal (two different locations); twelve alternatus Japan. DNA...
Research Highlights: Pestalotiopsis pini sp. nov. is an emerging pathogen on stone pine, Pinus pinea L., in Portugal. Background and Objectives: Stone pine one of the most important forest tree species Portugal whole Mediterranean basin. are common endophytes, saprobes or pathogens a variety hosts environments. The objective present study was to identify associated with symptomatic trees. Materials Methods: Samples trees showing shoot blight stem necrosis were obtained from orchards urban...
Abstract Platypus cylindrus is an important wood borer of cork oak trees ( Quercus suber ) in the Mediterranean region, namely Portugal, Morocco and Algeria where its presence has drastically increased past few decades. On contrary, insect not a relevant pest Tunisia. The aim this work to analyze morphological genetic differences among Tunisian Portuguese populations order understand their role diverse population dynamics (e.g., aggressiveness) insect. information could be used as novel tool...
Survival and development of Bursaphelenchus xylophilus (pine wood nematode) was studied for up to 40 weeks in Pinus pinaster sawn branches: 30 boards (1200 × 100 25 mm), long‐blocks 95 10 pine branches with bark nine without mm long). The nematode found all materials through the entire sampling period, higher abundance (boards long‐blocks). In initial period B. reproduced abundantly a population peak detected at 8–12 weeks. Subsequently, populations declined became dominated by third‐stage...
Biscogniauxia mediterranea is a widespread fungus that causes charcoal disease on cork oak and other hardwood hosts. It had been considered secondary pathogen causing the only in stressed However, its frequency severity have increasing, inclusive young trees without decline signs developing atypical symptoms. The present work aims to assess fungus' variability Mediterranean basin, following geographical distribution. A collection of 36 isolates originated from Portugal countries, hosts with...
Chemical compounds from leaves of fruit-producing trees, a waste agricultural activity can be isolated and used as source natural bioactive chemicals. Boiling water was an extractant Actinidia deliciosa co-extracted fibres were removed with ethanol precipitation. Rutin quercitrin the main flavonoids identified quantified by RP-HPLC-DAD. No cytotoxicity detected for any extracts towards Caco- 2 cell line. A permeation approx. 14% extract components through cells monolayer determined. The...
Charcoal canker, caused by the fungus Biscogniauxia mediterranea , is one of most frequent diseases cork oak in Portugal. The pathogen has been considered a secondary invader that attacks only stressed hosts; however, recent years, an increasing number young trees exhibiting disease symptoms have recorded. A collection monoascosporic cultures isolated from single stromata B. different locations was analyzed means microsatellite—Primed Polymerase Chain Reaction—using three microsatellite...
Abstract The fungus Sydowia polyspora is frequently isolated from conifers worldwide and considered a pathogen on several hosts. Stone pine ( Pinus pinea ) one of the most important forestry species throughout Mediterranean basin due to value edible nut. pines showing tip dieback, needles with tan‐ yellow‐coloured lesions shoot death, observed in stands Portugal, were sampled for analysis. Fungal colonies covered cream‐coloured spore masses, consistently obtained. Morphological phylogenetic...
The risks of Bursaphelenchus xylophilus (pine wood nematode) transfer in relation to material were assessed. Combinations infested and non‐infested adjacent boards, long‐blocks blocks Pinus pinaster , simulating assembled pallets, For the recipient wood, pieces with natural moisture content (MC), heat‐treatment (56°C for 30 min core) kiln‐drying <20% MC tested, along in‐service boards from pallets. Donor materials kept direct contact at 25°C or 10°C, nine replicates per treatment. was...
Platypus cylindrus is a pest that since the 80’s of last century has been considered cork oak mortality agent in Portugal. It an ambrosia beetle establishes complex symbioses with fungi whose role insect-fungus-host interaction not completely clarified. In order to characterize P. associated micoflora Portugal, were isolated from different organs and its galleries trees. Fungi genera Acremonium, Aspergillus, Beauveria, Botrytis, Chaetomium, Fusarium, Geotrichum, Gliocladium, Nodulisporium,...
Six entomopathogenic fungal isolates were tested under controlled conditions, as biological control agents against Monochamus galloprovincialis (Coleoptera: Cerambycidae) (Olivier), the vector of pine wood nematode Bursaphelenchus xylophilus (Nematoda: Parasitaphelenchidae) (Steiner and Buhrer). The fungi Beauveria bassiana (Bals.-Criv.) Vuill, Metarhizium anisopliae (Metchnikoff) Sorokin, sp. Fusarium larvae adults insect, by comparing two exposure techniques: continuous contact spraying....