A5060371415- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Rabies epidemiology and control
- RNA Interference and Gene Delivery
- Heme Oxygenase-1 and Carbon Monoxide
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Parasitic Infections and Diagnostics
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- interferon and immune responses
- Advanced biosensing and bioanalysis techniques
- Antifungal resistance and susceptibility
- Immune Response and Inflammation
- Fungal Infections and Studies
- Ocular Diseases and Behçet’s Syndrome
- Transplantation: Methods and Outcomes
- Malaria Research and Control
- Sex work and related issues
- Congenital Anomalies and Fetal Surgery
- Vector-borne infectious diseases
- Bartonella species infections research
- Microbial infections and disease research
- Virus-based gene therapy research
Instituto Adolfo Lutz
2012-2023
Secretaria da Saúde
2023
Faculdade de Medicina de São José do Rio Preto
2011
Hospital de Base
2011
This study analyzed the synthesis of Interferon gamma (IFN-γ), Tumor Necrosis Factor alpha (TNF-α), and Interleukin 10 (IL-10) in chronically infected patients which developed symptomatic disease as cerebral or ocular toxoplasmosis. Blood from 61 individuals were divided into four groups: Cerebral toxoplasmosis/AIDS (CT/AIDS group) (n = 15), toxoplasmosis (OT 23), chronic (CHR 13) healthy (HI 10). OT, CHR, HI groups human immunodeficiency virus (HIV) seronegative. The diagnosis was made by...
SUMMARY The aim of this study was to investigate risk factors for ocular toxoplasmosis (OT) in patients who received medical attention at a public health service. Three hundred and forty-nine consecutive patients, treated the Outpatient Eye Clinic Hospital de Base, São José do Rio Preto, Paulo state, Brazil, were enrolled study. After an eye examination, enzyme-linked immunosorbent assay (ELISA) used determine anti- Toxoplasma gondii antibodies. results showed that 25·5% seronegative 74·5%...
To determine the prevalence of asymptomatic cryptococcal antigen (CRAG) using lateral flow assay (LFA) in hospitalised HIV-infected patients with CD4 counts <200 cells/μl.Hospitalised were prospectively recruited at Instituto de Infectologia Emilio Ribas, a tertiary referral hospital to serving São Paulo State, Brazil. All >18 years old without prior meningitis, clinical suspicion regardless antiretroviral (ART) status, and cells/μl. Serum CRAG was tested by LFA all patients, whole blood...
Despite the development of serological and molecular methods in recent years, diagnosis cerebral toxoplasmosis human immunodeficiency virus (HIV)-infected patients still presents difficulties. In present study, we investigated whether induced changes reactivity serum toward Toxoplasma gondii excreted-secreted antigens (ESA) order to develop an assay for evaluating HIV-infected with toxoplasmosis. The antigen selection was based on those produced by tachyzoites, since it is form organism...
Aim This study analyzed microvesicles and exosomes, called as extracellular vesicles (EVs) excreted in serum cerebrospinal fluid (CSF) from patients with cerebral or gestational toxoplasmosis. Methods Clinical samples 83 individuals were divided into four groups. Group I, 20 sera healthy pregnant women (seronegative for toxoplasmosis); group II, 21 seropositive toxoplasmosis (cerebral forms); III, 26 CSF toxoplasmosis/HIV co-infection (CT/HIV) (seropositive IV, 16 seronegative toxoplasmosis,...
Ocular toxoplasmosis (OT) is one of the most common manifestations Toxoplasma gondii infection and can be related with congenital or acquired infections. OT cause posterior uveitis that serious sequelae as complete loss vision. microRNAs (miRNAs) are small non-coding RNAs, which have regulatory roles in cells by silencing messenger RNA. This study evaluated gene expression miR-155-5p, miR-146a-5p, miR-21-5p, miR-29c-3p miR-125b-5p plasma 51 patients ocular (OT Group), 26 individuals...
In Brazil, congenital anomalies rank second in causes of infant deaths and can be associated with environmental genetic factors, maternal conditions. The study objective was to investigate the risk profile development children malformations state Roraima. A total 158 women attended at Ambulatory Women's Health Reference Center Follow-up Hospital Materno Infantil Nossa Senhora Nazareth, Boa Vista, RR, Brazil selected. An epidemiological data sheet related factors that have relation bear...
Objective: To present a clinical case of pregnant woman who consistently showed low avidity IgG test results for Toxoplasma gondii in two consecutive pregnancies. Case details: G5P2A2, 41 years old, followed up the High-Risk Prenatal Care due to serologies T. with IgM+/IgG+ and performed at 11 weeks gestation, showing 49% avidity. She brought exams from previous pregnancy that also (42%). The fetus was evaluated Fetal Medicine outpatient clinic no ultrasonographic abnormalities. progressed...
Cerebral toxoplasmosis is the most common neurologic opportunistic infection in HIV-infected patients. Excretory-secretory antigens (ESA) are majority of circulating sera from hosts with acute toxoplasmosis, and their usefulness as has been shown. This study considered whether it could find anti-ESA antibodies cerebrospinal fluid (CSF) these can be markers active infection. Samples CSF 270 patients were analyzed divided into 3 groups according to presence or absence toxoplasmosis. Group I:...
Vero cells have been used successfully in Toxoplasma gondii maintenance. Medium supplementation for culture with fetal bovine serum is necessary cellular growth. However, these cultures presents disadvantages, such as the potential to induce hypersensitivity, variability of batches, possible presence contaminants, and high cost good quality serum. Culture media formulated without any animal derived components, designed serum-free growth cell lines different virus replication. The advantages...
Symptomatic forms of toxoplasmosis are a serious public health problem and occur in around 10-20% the infected people. Aiming to improve molecular diagnosis symptomatic Brazilian patients, this study evaluated performance real time PCR testing two primer sets (B1 REP-529) detecting Toxoplasma gondii DNA. The methodology was assayed 807 clinical samples with known diagnosis, ELISA, conventional results 9-year period. All were from patients suspicion several features toxoplasmosis. According...
Toxoplasmosis was recently included as a neglected disease by the Center for Disease Control. Ocular toxoplasmosis is one clinical presentation of congenital or acquired infection. The laboratory diagnosis being used worldwide to support and imaging. aim this study evaluate use serology molecular methods monitor acute OT in immunocompetent patients during treatment. Five were clinically diagnosed with OT. evaluation performed ophthalmologic examination using Early Treatment Diabetic...
Cryptococcal meningitis is the most common cause of opportunistic in HIV-infected patients Brazil and causes unacceptable high mortality rates. In this study, with a first episode culture-proven cryptococcal cerebrospinal fluid (CSF) were prospectively included order to evaluate sensitivity antigen (CrAg) lateral flow assay (LFA) serum, CSF, whole blood (fingerstick), fresh urine. addition, other neurological confirmed diseases specificity CrAg LFA serum. Twenty 19 them, positive (95%...
This study investigated the potential of five miRNA candidates for cerebral toxoplasmosis/HIV co-infection (CT/HIV) biomarkers. miR-155-5p, miR-146a-5p, miR-21-5p, miR-125b-5p and miR-29c-3p were tested in 79 plasma divided into groups: 32 CT/HIV patients; 27 individuals with asymptomatic toxoplasmosis (AT); 20 seronegative (NC). From each was collected peripheral blood/EDTA laboratory diagnosis. Blood cells DNA extractions (molecular diagnosis), RNA (gene expression) ELISA (serological...
Toxoplasmosis transmission during pregnancy can cause severe sequelae in fetuses and newborns. Maternal antibodies may be indicators of risk or immunity. The aim here was to evaluate seropositivity for anti-Toxoplasma gondii (anti-T. gondii) immunoglobulin M (IgM) G (IgG) IgG avidity pregnant women their newborn infants.
This study evaluated levels for mRNA expression of 7 cytokines in ocular toxoplasmosis. Peripheral blood mononuclear cells (PBMC) patients with toxoplasmosis (OT Group, n = 23) and chronic individuals (CHR 9) were isolated stimulated vitro T. gondii antigen. Negative controls (NC) constituted PBMC samples from seronegative was determined by qPCR. Results showed a significant increase antigen PBMCs derived OT Group expressing IL-6 (at P < .005 .0005 CHR NC groups, respectively), IL-10...
ABSTRACT Background Ocular toxoplasmosis (OT) is a frequent clinical manifestation due to infection by Toxoplasma gondii. It characterized an inflammatory process involving macrophages activated pro-inflammatory cytokines. The expression of microRNAs takes place during the and, among them, miRNA 511 regulates activation macrophages. This study evaluated 511_5p in patients with OT and healthy controls. Methods A total 361 from Hospital de Base Fundação Faculdade Medicina São José do Rio Preto...