A5058254314- DNA and Nucleic Acid Chemistry
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Plant tissue culture and regeneration
- Bacterial Genetics and Biotechnology
- Force Microscopy Techniques and Applications
- Chromosomal and Genetic Variations
- RNA Interference and Gene Delivery
- Neural Networks and Applications
- Potato Plant Research
- Metabolomics and Mass Spectrometry Studies
- Agricultural Science and Fertilization
- Graphene research and applications
- SARS-CoV-2 detection and testing
- RNA modifications and cancer
- Diffusion and Search Dynamics
- Plant Taxonomy and Phylogenetics
- 2D Materials and Applications
- Seed Germination and Physiology
- Nanowire Synthesis and Applications
- Security, Politics, and Digital Transformation
Imperial College London
2020-2025
Leiden University
2016-2024
MRC London Institute of Medical Sciences
2020-2024
Medical Research Council
2024
Northeastern University
2022
Huygens Institute for History and Culture of the Netherlands
2017-2021
Delft University of Technology
2016-2017
Heriot-Watt University
2015
Botanic Gardens and Parks Authority
2010
Curtin University
2010
Two-dimensional transition metal dichalcogenide semiconductors are intriguing hosts for quantum light sources due to their unique optoelectronic properties. Here, we report that strain gradients, either unintentionally induced or generated by substrate patterning, result in spatially and spectrally isolated emitters mono- bilayer WSe2. By correlating localized excitons with variations, show the emitter emission energy can be red-tuned up a remarkable ∼170 meV. We probe fine-structure,...
Homologous recombination (HR) is an essential DNA double-strand break (DSB) repair mechanism, which frequently inactivated in cancer. During HR, RAD51 forms nucleoprotein filaments on RPA-coated, resected and catalyzes strand invasion into homologous duplex DNA. How displaces RPA assembles long HR-proficient remains uncertain. Here, we employed single-molecule imaging to investigate the mechanism of nematode RAD-51 filament growth presence BRC-2 (BRCA2) paralogs, RFS-1/RIP-1. nucleates...
Torsional stress generated during DNA replication and transcription has been suggested to facilitate nucleosome unwrapping thereby the progression of polymerases. However, propagation twist in condensed chromatin remains yet unresolved. Here, we measure how force torque impact fibers with a repeat length 167 197. We find that both types fold into left-handed superhelix can be stabilized by positive torsion. observe structural changes induced were reversible, indicating large degree...
Abstract DNA double-stranded breaks (DSBs) are deleterious lesions, and their incorrect repair can drive cancer development 1 . HELQ is a superfamily 2 helicase with 3′ to 5′ polarity, its disruption in mice confers germ cells loss, infertility increased predisposition ovarian pituitary tumours 2–4 At the cellular level, defects result hypersensitivity cisplatin mitomycin C, persistence of RAD51 foci after damage 3,5 Notably, binds RPA RAD51-paralogue BCDX2 complex, but relevance these...
Homologous recombination (HR) is essential for error-free repair of DNA double-strand breaks, perturbed replication forks (RFs), and post-replicative single-stranded (ssDNA) gaps. To initiate HR, the mediator tumor suppressor protein BRCA2 facilitates nucleation RAD51 on ssDNA prior to stimulation filament growth by paralogs. Although binding has been implicated in nucleation, function double-stranded (dsDNA) remains unclear. Here, we exploit single-molecule (SM) imaging visualize...
DNA crosslinks block replication and are repaired by the Fanconi anaemia pathway. The FANCD2-FANCI (D2-I) protein complex is central to this process as it initiates repair coordinating incisions around lesion
Abstract Nucleosome-nucleosome interactions drive the folding of nucleosomal arrays into dense chromatin fibers. A better physical account fibers is necessary to understand role in regulating DNA transactions. Here, we studied unfolding pathway regular as a function single base pair increments linker length, using both rigid base-pair Monte Carlo simulations and single-molecule force spectroscopy. Both computational experimental results reveal periodic variation energies due limited...
Abstract Resolution of Holliday junctions is a critical intermediate step homologous recombination in which are processed by junction-resolving endonucleases. Although binding and cleavage well understood, the question remains how enzymes locate their substrate within long duplex DNA. Here we track fluorescent dimers endonuclease I on DNA, presenting complete single-molecule reaction trajectory for enzyme finding cleaving junction. We show that binds remotely to dsDNA then undergoes 1D...
Here, we describe a rapid and versatile protocol to generate gapped DNA substrates for single-molecule (SM) analysis using optical tweezers via site-specific Cas9 nicking force-induced melting. We provide examples of single-stranded (ss) gaps different length position. outline protocols visualize these by replication protein A-enhanced Green Fluorescent Protein (RPA-eGFP) SYTOX Orange staining commercially available (C-TRAP). Finally, demonstrate the utility SM bidirectional growth...
ABSTRACT Homologous recombination (HR) is an essential DNA double-strand break (DSBs) repair mechanism frequently inactivated in cancer. During HR, RAD51 forms nucleoprotein filaments on RPA-coated resected and catalyses strand invasion into homologous duplex DNA. How displaces RPA assembles long HR-proficient remains uncertain. Here, we employ single-molecule imaging to investigate the of nematode RAD-51 filament growth presence BRC-2 (BRCA2) paralogs, RFS-1/RIP-1. nucleates DNA, while...
Abstract Resolution of four-way Holliday junctions is a critical intermediate step homologous recombination. DNA must be processed by abundant junction-resolving endonucleases to cleave the covalent link between two chromosomes. Although catalytic activity and interaction mode with junction have been characterized in great detail biochemical structural studies, it remains unclear how enzymes find their substrate located within kilobase pairs duplex DNA. Here, we present complete...