Angiogenesis has an important function in the prolife­ration and metastasis of hepatocellular carcinoma (HCC) under a hypoxic tumor microenvironment. Activated hepatic stellate cells (HSCs) infiltrate stroma liver tumors potently increase angiogenesis through tumor‑stromal interactions, however, exact mechanism by which this occurs is unknown. The present study aimed to investigate paracrine effects HCC‑derived platelet‑derived growth factor‑BB (PDGF‑BB) on HSCs conditions. It was...

Cancer is not only influenced by specific tumor cells but also the stromal microenvironment. Upon liver damage, activated hepatic stellate (aHSC) become highly proliferative myofibroblast-like and are thought to secrete molecules that influence development of hepatocellular carcinoma (HCC). The aim this study was investigate role aHSC in HCC.To assess if secreted factor(s) promote microvascular endothelial cell (MEC) tube formation, MEC were plated with aHSC-conditioned medium formation...

Dynamic demand prediction is a key issue in ride-hailing dispatching. Many methods have been developed to improve the accuracy of an increase demand-responsive, transport services. However, uncertainties predicting demands due multiscale spatiotemporal granularity, as well resulting statistical errors, are seldom explored. This paper attempts fill this gap and examine granularity effects on by using empirical data for Chengdu, China. A convolutional, long short-term memory model combined...

Abstract Backgound Pancreatic cancer (PC) is one of the deadliest cancers worldwide, and cell proliferation angiogenesis play an important role in its occurrence development. High levels lncRNANORAD have been detected many tumors, including PC, yet effect mechanism lncRNA NORAD on PC are unexplored. Methods qRT.PCR was applied to quantify miR-532-3p expression cells, a dual luciferase reporter gene used verify targeting effects NORAD, Nectin-4. Then, we regulated cells their using cloning...

Global climate change leads to frequent extreme snowfall weather, which has a significant impact on the safety and operating efficiency of urban public transportation. In order cope with adverse effects governments should vigorously develop sustainable Since transportation is critical component building city, traffic management departments quantitatively analyze performance changes network under weather conditions. Therefore, fully considering comprehensive topology improve robustness...

Lipid nanoparticle (LNP) delivery systems are widely used in the of small-molecule drugs and nucleic acids. In this study, we prepared LNP-miR-155 by lipid nanomaterial technology investigated effects on β-catenin/transcription factor 4 (TCF4)/solute carrier family 31 member 1/copper transporter 1 (SLC31A1/CTR1) signaling copper transport colorectal cancer. For this, an cy5 inhibitor mimics for transfection HT-29/SW480 cells. The efficiency uptake were detected immunofluorescence. Relevant...

Colorectal cancer (CRC) is the third most common form of malignant tumor and characterized by high rates proliferation metastases. Circular RNAs (circRNAs) are a noncoding closed loop RNA molecules play vital roles in progression various types humans. Here, we used circRNA microarray sequencing technology to analyze different circRNAs between CRC tissues normal explore role circIFT80 colorectal cancer. In this present study, found that was abnormally overexpression cells. While knockout HT29...

Increasing evidence shows that circular RNAs (circRNAs) play a key role in the development of colorectal cancer (CRC). An interesting candidate RNA this context is hsa-circRNA-0067835 (circIFT80), but its network actions still unclear.Big data mining technology was used to explore downstream microRNAs (miRNA) and messenger (mRNA) circIFT80 network. A regulatory network, comprising corresponding miRNAs mRNAs, derived preliminarily potential mechanism CRC. Finally, proposed experimentally...

The accurate short-term travel demand predictions of ride-hailing orders can promote the optimal dispatching vehicles in space and time, which is crucial issue to achieve sustainable development such dynamic demand-responsive service. sparse demands are always ignored previous models, uncertainties spatiotemporal distribution induced by setting subjective thresholds rarely explored. This paper attempts fill this gap examine sparsity effect on prediction using Didi Chuxing order data recorded...

Glucose is an important energy source for tumors, however the molecular mechanisms by which tumor cells regulate glucose uptake remain unclear. In this study, we aimed to investigate regulation mechanism of WNT7B/β-catenin pathway transporter 1 (GLUT1)-mediated metabolism in colorectal cancer. Here, found that WNT7B expression levels were significantly increased cancer tissues and closely associated with clinical stage lymph node metastasis patients Next, confirmed consumption lactic acid...

Background: Emerging evidence indicates the essential role of cancer stem cells (CSCs) in development and progression various cancers, including colorectal (CRC). CELF6, a member cytosine-uridine-guanine-binding protein (CUG-BP), Elav-like family (CELF), has been reported to be downregulated CRC tissues. This study aims elucidate underlying mechanisms CELF6 progression. Methods: The expression levels prognostic significance along with its association homeobox A5 (HOXA5), were analyzed using...

It is well known that neoadjuvant radiotherapy could reduce local recurrence followed by surgical resection. However, evidence about oncologic efficacy of and survival benefit adjuvant chemotherapy after still lacking.This retrospective propensity score-matched cohort study identified patients with pathologically confirmed rectal cancer receiving surgery curative intent from the Surveillance, Epidemiology, End Results database 2004 through 2014. Overall was compared using stratified log-rank...

Abstract Pancreatic ductal adenocarcinoma, one of the deadliest tumors digestive tract, is a difficult and invasive malignancy. Current treatment for pancreatic adenocarcinoma mainly depends on surgery combined with radiotherapy chemotherapy, which, however, often resulting in questionable curative effect. Therefore, new targeted therapies are needed future treatment. We first interfered hsa_circ_0084003 expression cells, further studied how functioned regulating cell aerobic glycolysis...

Objective To investigate the application effect of seamless nursing mode in emergency department. Methods Totally 211 cases patients admitted Emergency Department Sichuan Province Panzhihua Central Hospital from August 2014 to November 2015 were selected and divided into experimental group (n=106) control (n=105) according admission time order. Patients received mode, while routine model. The incidence rate adverse reactions, mortality, hospitalization time, depression anxiety,...

Objective To investigate the effect of activated hepatic stellate cells(HSC) to angiogenesis, proliferation and metastasis hepatocellular carcinoma (HCC). Methods Thirty BALB/c-nu female nude mice were divided into 3 groups according randomly digital table: normal control group, low consistency group high with 10 in each group. Mice received an intrahepatic injection 1×106 hepatoma cells(H22), H22 1×105 HSC-T6 cells labeled by (5, 6-carboxyfluorescein diacetate, succinimidyl ester,...

Objective To investigate the correlation between human hepatocellular carcinoma associated fibroblast (hCAF) and angiogenesis in (HCC) tissues. Methods Twenty patients who underwent radical hepatectomy Third Affiliated Hospital of Sun Yat-sen University January 2014 May were enrolled this prospective study. Among them, 16 males 4 females, aged 35-58 years old with a median age 42 old. The informed consents all obtained local ethical committee approval was received. Clinical...