A5091489138- Protein Structure and Dynamics
- Enzyme Structure and Function
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Protein Interaction Studies and Fluorescence Analysis
- Enzyme Catalysis and Immobilization
- Microbial Fuel Cells and Bioremediation
- Pharmacological Effects of Natural Compounds
- Folate and B Vitamins Research
- Alzheimer's disease research and treatments
- Protease and Inhibitor Mechanisms
- RNA Interference and Gene Delivery
- Membrane-based Ion Separation Techniques
- Peptidase Inhibition and Analysis
- Protein purification and stability
- Estrogen and related hormone effects
- Endoplasmic Reticulum Stress and Disease
- Membrane Separation Technologies
- Mitochondrial Function and Pathology
- SARS-CoV-2 and COVID-19 Research
- Cancer, Stress, Anesthesia, and Immune Response
- Biochemical Acid Research Studies
- Aldose Reductase and Taurine
- Phytochemicals and Medicinal Plants
- Metabolism and Genetic Disorders
Manipal University Jaipur
2020-2024
University of Toledo
2014-2024
Invertis University
2017-2022
Rice University
2012-2014
Jamia Millia Islamia
2005-2011
We report the effects of stabilizing osmolytes (low molecular mass organic compounds that raise midpoint thermal denaturation) on stability and function RNase-A under physiological conditions (pH 6.0 25 degrees C). Measurements Gibbs free energy change at C (DeltaG(D) ) kinetic parameters, Michaelis constant (K(m)) catalytic (k(cat)) enzyme mediated hydrolysis cytidine monophosphate, enabled us to classify into three different classes based their parameters protein stability. (a) Polyhydric...
Abstract Background The world has been battling several vector-borne diseases since time immemorial. Socio-economic marginality, precipitation variations and human behavioral attributes play a major role in the proliferation of these diseases. Lockdown social distancing have affected aspects life somehow impact on spread vector borne This article sheds light into relationship between COVID-19 lockdown global dengue burden with special focus India. It also focuses interconnection pandemic...
Abstract Chlorpyrifos (CPF) is an extensively used organophosphate pesticide for crop protection. However, there are concerns of it contaminating the environment and human health with estimated three lakh deaths annually. Detection CPF in blood samples holds significance to avoid severe outcomes due continuous exposure. The most common techniques detection Gas chromatography (GC) high-performance liquid (HPLC). these might not be feasible at community healthcare level high-cost...
We directly measure the dynamics of HIV trans-activation response (TAR)-DNA hairpin with multiple loops using single-molecule Förster resonance energy transfer (smFRET) methods. Multiple FRET states are identified that correspond to intermediate melting hairpin. The stability each state is calculated from smFRET data. results indicate unfolding obeys a "fraying and peeling" mechanism, evidence for collapse ends during folding observed. These suggest possible biological function serving as...
Alzheimer's disease, categorized by the piling of amyloid-β (Aβ), hyperphosphorylated tau, PHFs, NFTs and mTOR hyperactivity, is a neurodegenerative disorder, affecting people across globe. Osmolytes are known for osmoprotectants play pivotal role in protein folding, function stability, thus, preventing proteins aggregation, counteracting effects denaturing solutes on proteins. (viz., sorbitol, inositol, betaine) perform maintaining homeostasis during hyperosmotic stress. The selective...
Lengthening smFRET lifetimes: We investigated various photoprotection system combinations to find the combination that optimally extended photobleach lifetime of a Cy3/Cy5 pair attached DNA hairpin in single-molecule environment. found glucose/glucose oxygen-scavenging solution with redox-based photostabilization solutions yielded longest average photobleaching lifetimes. As service our authors and readers, this journal provides supporting information supplied by authors. Such materials are...
Osmolytes are small organic molecules that known to stabilize proteins and other biological macromolecules under various stressful conditions. They belong categories such as amino acids, methylamines, polyols. These substances commonly 'compatible solutes' because they do not disrupt cellular processes help regulate the osmotic balance within cells. In case of ribonuclease A (RNase A), which is prone aggregation, presence osmolytes can maintain its structural stability prevent unwanted...
Osmolytes are small organic compounds accumulated at higher concentrations in the cell under various stress conditions like high temperature, salt, pressure, etc. mainly include four major classes of including sugars, polyols, methylamines, and amino acids their derivatives. In addition to ability maintain protein stability folding, these osmolytes, also termed as chemical chaperones, can prevent misfolding aggregation. Although being efficient folders stabilizers, osmolytes exhibit certain...