A5087877892- Protein Structure and Dynamics
- Enzyme Structure and Function
- Redox biology and oxidative stress
- Metabolism and Genetic Disorders
- Folate and B Vitamins Research
- Heat shock proteins research
- Endoplasmic Reticulum Stress and Disease
- Protein purification and stability
- Advanced Glycation End Products research
- Computational Drug Discovery Methods
- Enzyme Catalysis and Immobilization
- Cholinesterase and Neurodegenerative Diseases
- Glutathione Transferases and Polymorphisms
- Muscle metabolism and nutrition
- Alzheimer's disease research and treatments
- Bacterial Infections and Vaccines
- Sulfur Compounds in Biology
- Medicinal Plants and Neuroprotection
- Reproductive tract infections research
- Peptidase Inhibition and Analysis
- Phytochemicals and Medicinal Plants
- Porphyrin Metabolism and Disorders
- Insect and Pesticide Research
- Biomedical Research and Pathophysiology
- Seed and Plant Biochemistry
University of Delhi
2015-2024
Ambedkar University Delhi
2013-2023
ICAR - Central Island Agricultural Research Institute
2021
Jamia Millia Islamia
2010
Fox Chase Cancer Center
2008-2009
The extracellular protein, transthyretin is responsible for the transport of thyroxin and retinol binding protein complex to various parts body. In addition this function, has also been involved in cardiovascular malfunctions, polyneuropathy, psychological disorders, obesity diabetes, etc. Recent developments have evidenced that associated with many other biological functions are directly or indirectly oxidative stress, common hallmark human diseases. review, we attempted address stress...
We report the effects of stabilizing osmolytes (low molecular mass organic compounds that raise midpoint thermal denaturation) on stability and function RNase-A under physiological conditions (pH 6.0 25 degrees C). Measurements Gibbs free energy change at C (DeltaG(D) ) kinetic parameters, Michaelis constant (K(m)) catalytic (k(cat)) enzyme mediated hydrolysis cytidine monophosphate, enabled us to classify into three different classes based their parameters protein stability. (a) Polyhydric...
Understanding of protein structure and stability gained to date has been acquired through investigations made under dilute conditions where total macromolecular concentration never surpasses 10 g l−1. However, biological macromolecules are known evolve function crowded intracellular environments that comprises proteins, nucleic acids, ribosomes carbohydrates etc. Crowded environment is result in altered properties including thermodynamic, structural functional aspect as compared the present...
Cystathionine beta-synthase (CBS), a key enzyme in the metabolism of homocysteine, has previously been shown to require heme co-factor for maximal activity. However, biochemical function CBS is not well defined. Here, we show that expression human heme-deficient strains Saccharomyces cerevisiae and Escherichia coli results production an misfolded degraded. Addition exogenous heme, porphyrins with non-iron metal, or porphyrin lacking metal entirely produced stable active enzyme. Purification...
Most of the human diseases related to various proteopathies are confined brain, which leads development forms neurological disorders. The brain consists several osmolytic compounds, such as N-Acetylaspartate (NAA), myo-inositol (mI), glutamate (Glu), glutamine (Gln), creatine (Cr), and choline-containing compounds (Cho). Among these osmolytes, level NAA drastically decreases under conditions, and, hence, is considered be one most widely accepted neuronal biomarkers in To date, no data...
Peroxiredoxins(Prdx), the family of non-selenium glutathione peroxidases, are important antioxidant enzymes that defend our system from toxic reactive oxygen species (ROS). They thiol-based peroxidases utilize self-oxidation their peroxidatic cysteine (Cp) group to reduce peroxides and peroxidized biomolecules. However, because its high affinity for hydrogen peroxide this moiety is extremely susceptible hyperoxidation, forming peroxidase inactive sulfinic acid (Cys-SO2H) sulfonic (Cys-SO3H)...
Abstract Peroxiredoxin 6 (Prdx6) is a ubiquitously expressed antioxidant non-selenium glutathione peroxidase that known to play major role in various physiological and pathological processes. It belongs the family of peroxidases (referred as Peroxiredoxins, Prdx’s) work independently any prosthetic groups or co-factors, instead utilize peroxidatic thiol residue for peroxide reduction. Mammalian Prdx’s are classified according number Cys implicated their catalytic activity by formation either...
Peroxiredoxin 6 (Prdx6), the ubiquitously expressed enzyme belonging to family of peroxidases, namely, peroxiredoxins, exhibits a unique feature functional compartmentalization within cells. Whereas, localized in cytosol shows glutathione peroxidase activity, its lysosomal counterpart performs calcium independent phospholipase A2 (aiPLA2) activity. Like any true moonlighting protein, these two activities Prdx6 are mutually exclusive each other as function pH cellular compartments....
Alzheimer's disease (AD) is a progressive neurological disorder affecting an estimated 10 million people worldwide. There no cure for AD, and only handful of drugs are known to provide some relief the symptoms. The prescription drug donepezil has been widely used treat slow progression onset disease; however, unpleasant side effects have paved way find alternative medicines. Many herbs improve brain function, but evidence medicinal plants that can AD limited due lack concrete rational...