Over the last two decades, surface plasmon resonance (SPR) sensors have advanced significantly, becoming an important tool in disciplines such as biosensing, chemical sensing, and material characterization. SPR has gained popularity biosensing because of its great sensitivity specificity detecting biomolecular interactions. This review provides overview recent developments biosensor technology applications medical diagnostics. To provide up-to-date area, includes most works from decade....

Glucose, the primary substrate for ATP synthesis, is catabolized during glycolysis to generate and precursors synthesis of other vital biomolecules. Opportunistic viruses cancer cells often hijack this metabolic machinery obtain energy components needed their replication proliferation. One way halt such energy-dependent processes by interfering with glycolytic pathway. 2-Deoxy-d-glucose (2-DG) a synthetic glucose analogue that can inhibit key enzymes in The efficacy 2-DG has been reported...

Rapidly progressing antibiotic resistance is a great challenge in therapy. In particular, the infections caused by carbapenem-resistant Enterobacteriaceae (CRE) are exceedingly difficult to treat. Carbapenemase production predominant mechanism of CRE. Early and accurate identification carbapenemase-producing (CP-CRE) extremely important for treatment prevention such infections. present study, four phenotypic carbapenemase detection tests were compared an algorithm was developed rapid...

Canavan disease (CD) is a fatal, childhood neurological disorder caused by mutations in the ASPA gene, leading to catalytic deficiencies aspartoacylase (ASPA) enzyme and impaired N-acetyl-l-aspartic acid metabolism brain. To study possible structural defects triggered these mutations, four missense associated with different severities have been structurally characterized. These mutant enzymes each overall structures similar that of native enzyme, but varying degrees alterations offer...

Background Diabetes mellitus type 2 (DMT2) is a leading metabolic disorder in the world. Anti-diabetic actions of phytochemicals from various medicinal herbs have been explored as an alternative therapy management DMT2 due to adverse effects synthetic drugs used allopathic medicine. α-amylase and α-glucosidase inhibitory potential phytochemical profiling were investigated aqueous extracts two new Cinnamomum zeylanicum accessions, namely C. Sri Wijaya (SW), Gemunu (SG) commercially available...

Extended-spectrum β-lactamase producing Enterobacteriaceae (ESBL-PE) and carbapenem-resistant (CRE) are disseminated worldwide posing a serious public health concern. Although, the presence of ESBL-PE CRE in Sri Lanka has been reported, prevalence is unknown. This study aimed to provide up-to-date epidemiological data on multidrug-resistant characterize molecular determinants carbapenemase-producing (CPE) Lanka.A prospective cross-sectional was conducted at tertiary care hospital between...

Osmolytes are small organic compounds accumulated at higher concentrations in the cell under various stress conditions like high temperature, salt, pressure, etc. mainly include four major classes of including sugars, polyols, methylamines, and amino acids their derivatives. In addition to ability maintain protein stability folding, these osmolytes, also termed as chemical chaperones, can prevent misfolding aggregation. Although being efficient folders stabilizers, osmolytes exhibit certain...

S-Adenosyl-l-methionine (AdoMet) is an essential metabolite, serving in a very wide variety of metabolic reactions. The enzyme that produces AdoMet from l-methionine and ATP (methionine adenosyltransferase, MAT) thus attractive target for antimicrobial agents. We previously showed methionine analogues are MAT substrates, yielding function specific methyltransfer However, this left open the question whether modified molecules could support bacterial growth, meaning they functioned full range...

Bacteria use quorum sensing to probe and respond population densities in their external environment. The detection of signaling molecules causes a virulence response many pathogenic bacteria. Blocking this pathway, without interfering with critical metabolic functions, would produce compounds that can disarm pathogens killing them. By not blocking growth per se, therapeutic approach have lower associated risk for the development bacterial resistance. Modified forms l-methionine yield...

Bioactive compounds entrapped in plant materials can be effectively recovered using fungal enzymes. Cinnamomum zeylanicum Sri Wijaya (SW) and Gemunu (SG) accessions commercially available C. (CC) were subjected to pretreatment extracted with pressured water (PWE, 0·098 MPa). Thirteen species isolated the substrate utilization ability of was tested cellulose, pectin lignin (indirectly). Total phenolic content (TPC, Folin–Ciocalteu method), proanthocyanidin (PC, vanillin method) α-amylase...

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Canavan disease (CD) is a fatal neurodegenerative disorder with impaired N‐acetyl‐L‐aspartic acid (NAA) metabolism. This caused by mutations in the ASPA gene, leading to deficiency aspartoacylase (ASPA) enzyme that hydrolyzes NAA brain. The majority of defects are missense found remote from active site, resulting decreased stability and activity. To study possible structural perturbations triggered these mutations, mutants associated different severities have been cloned, purified,...

Canavan disease is a fatal neurological disorder caused by mutations in the aspA gene, leading to an altered aspartoacylase with diminished catalytic activity. These clinical are found at sites located throughout enzyme, majority far from catalytically important amino acids. This suggests that destabilization of protein structure may be responsible for loss activity enzyme A number different mutants have been cloned, expressed and purified. purified enzymes were low thermal ionic stability...